High-throughput immunomagnetic cell detection using a microaperture chip system

Chun Li Chang, Shadia I. Jalal, Wanfeng Huang, Aamer Mahmoud, Daniela E. Matei, Cagri A. Savran

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report a microchip system based on a combination of immunomagnetic separation, microfluidics, and size-based filtration for high-throughput detection of rare cells. In this system, target cells bind to magnetic beads in vitro and flow parallel to a microchip with flow rates of milliliters/minute. A magnetic field draws the bead-bound cells toward the microchip, which contains apertures that allow passage of unbound beads while trapping the target cells. The cells captured on the chip can be investigated clearly under a microscope and released from the chip for further analysis. We first characterize the system by detecting cancer cell lines (MCF-7 and A549) in culture media. We then demonstrate detection of 100 MCF-7 cells spiked in 7.5 mL of human blood to simulate detection of circulating tumor cells present in cancer patient blood samples. On average, 85% of the spiked cells were detected. We expect this system to be highly useful in a wide variety of clinical as well as other applications that seek rare cells.

Original languageEnglish
Article number6808412
Pages (from-to)3008-3013
Number of pages6
JournalIEEE Sensors Journal
Volume14
Issue number9
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Blood
chips
Cells
Throughput
Parallel flow
cells
Cell culture
Microfluidics
Tumors
Microscopes
Flow rate
beads
Magnetic fields
blood
cancer
culture media
parallel flow
cultured cells
tumors
flow velocity

Keywords

  • CTC detection
  • immunomagnetic separation
  • microfluidics
  • rare cell detection
  • size-based filtration
  • tumor cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Instrumentation

Cite this

Chang, C. L., Jalal, S. I., Huang, W., Mahmoud, A., Matei, D. E., & Savran, C. A. (2014). High-throughput immunomagnetic cell detection using a microaperture chip system. IEEE Sensors Journal, 14(9), 3008-3013. [6808412]. https://doi.org/10.1109/JSEN.2014.2321167

High-throughput immunomagnetic cell detection using a microaperture chip system. / Chang, Chun Li; Jalal, Shadia I.; Huang, Wanfeng; Mahmoud, Aamer; Matei, Daniela E.; Savran, Cagri A.

In: IEEE Sensors Journal, Vol. 14, No. 9, 6808412, 2014, p. 3008-3013.

Research output: Contribution to journalArticle

Chang, CL, Jalal, SI, Huang, W, Mahmoud, A, Matei, DE & Savran, CA 2014, 'High-throughput immunomagnetic cell detection using a microaperture chip system', IEEE Sensors Journal, vol. 14, no. 9, 6808412, pp. 3008-3013. https://doi.org/10.1109/JSEN.2014.2321167
Chang, Chun Li ; Jalal, Shadia I. ; Huang, Wanfeng ; Mahmoud, Aamer ; Matei, Daniela E. ; Savran, Cagri A. / High-throughput immunomagnetic cell detection using a microaperture chip system. In: IEEE Sensors Journal. 2014 ; Vol. 14, No. 9. pp. 3008-3013.
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