High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking

Ben Gary Harvey, Yael Strulovici-Barel, Thomas L. Vincent, Jason G. Mezey, Ramya Raviram, Cynthia Gordon, Jacqueline Salit, Ann E. Tilley, Augustine Chung, Abraham Sanders, Ronald Crystal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The distribution of lung disease induced by inhaled cigarette smoke is complex, depending on many factors. With the knowledge that the small airway epithelium (SAE) is the earliest site of smoking-induced lung disease, and that the SAE gene expression is likely sensitive to inhaled cigarette smoke, we compared upper vs. lower lobe gene expression in the SAE within the same cigarette smokers to determine if the gene expression patterns were similar or different. Active smokers (n = 11) with early evidence of smoking-induced lung disease (normal spirometry but low diffusing capacity) underwent bronchoscopy and brushing of the upper and lower lobe SAE in order to compare upper vs lower lobe genome-wide and smoking-responsive gene expression by microarray. Cluster and principal component analysis demonstrated that, for each individual, the expression of the known SAE smoking-responsive genes were highly correlated in upper and lower lobe pairs, although, as expected, there were differences in the smoking-induced changes in gene expression from individual to individual. These observations support the concept that the heterogeneity observed among smokers in the anatomic distribution of smoking-induced disease are not secondary to the topographic differences in the effects of cigarette smoke on the airway epithelium.

Original languageEnglish
Article numbere72669
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - 9 Sep 2013
Externally publishedYes

Fingerprint

Gene expression
epithelium
Epithelium
cigarettes
Smoking
Tobacco Products
Pulmonary diseases
Gene Expression
smoke
gene expression
Smoke
respiratory tract diseases
Lung Diseases
Genes
bronchoscopy
Spirometry
Bronchoscopy
Microarrays
Principal Component Analysis
Principal component analysis

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Harvey, B. G., Strulovici-Barel, Y., Vincent, T. L., Mezey, J. G., Raviram, R., Gordon, C., ... Crystal, R. (2013). High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking. PLoS One, 8(9), [e72669]. https://doi.org/10.1371/journal.pone.0072669

High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking. / Harvey, Ben Gary; Strulovici-Barel, Yael; Vincent, Thomas L.; Mezey, Jason G.; Raviram, Ramya; Gordon, Cynthia; Salit, Jacqueline; Tilley, Ann E.; Chung, Augustine; Sanders, Abraham; Crystal, Ronald.

In: PLoS One, Vol. 8, No. 9, e72669, 09.09.2013.

Research output: Contribution to journalArticle

Harvey, BG, Strulovici-Barel, Y, Vincent, TL, Mezey, JG, Raviram, R, Gordon, C, Salit, J, Tilley, AE, Chung, A, Sanders, A & Crystal, R 2013, 'High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking', PLoS One, vol. 8, no. 9, e72669. https://doi.org/10.1371/journal.pone.0072669
Harvey BG, Strulovici-Barel Y, Vincent TL, Mezey JG, Raviram R, Gordon C et al. High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking. PLoS One. 2013 Sep 9;8(9). e72669. https://doi.org/10.1371/journal.pone.0072669
Harvey, Ben Gary ; Strulovici-Barel, Yael ; Vincent, Thomas L. ; Mezey, Jason G. ; Raviram, Ramya ; Gordon, Cynthia ; Salit, Jacqueline ; Tilley, Ann E. ; Chung, Augustine ; Sanders, Abraham ; Crystal, Ronald. / High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking. In: PLoS One. 2013 ; Vol. 8, No. 9.
@article{714a45140c984df59dc28ae79ff20527,
title = "High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking",
abstract = "The distribution of lung disease induced by inhaled cigarette smoke is complex, depending on many factors. With the knowledge that the small airway epithelium (SAE) is the earliest site of smoking-induced lung disease, and that the SAE gene expression is likely sensitive to inhaled cigarette smoke, we compared upper vs. lower lobe gene expression in the SAE within the same cigarette smokers to determine if the gene expression patterns were similar or different. Active smokers (n = 11) with early evidence of smoking-induced lung disease (normal spirometry but low diffusing capacity) underwent bronchoscopy and brushing of the upper and lower lobe SAE in order to compare upper vs lower lobe genome-wide and smoking-responsive gene expression by microarray. Cluster and principal component analysis demonstrated that, for each individual, the expression of the known SAE smoking-responsive genes were highly correlated in upper and lower lobe pairs, although, as expected, there were differences in the smoking-induced changes in gene expression from individual to individual. These observations support the concept that the heterogeneity observed among smokers in the anatomic distribution of smoking-induced disease are not secondary to the topographic differences in the effects of cigarette smoke on the airway epithelium.",
author = "Harvey, {Ben Gary} and Yael Strulovici-Barel and Vincent, {Thomas L.} and Mezey, {Jason G.} and Ramya Raviram and Cynthia Gordon and Jacqueline Salit and Tilley, {Ann E.} and Augustine Chung and Abraham Sanders and Ronald Crystal",
year = "2013",
month = "9",
day = "9",
doi = "10.1371/journal.pone.0072669",
language = "English",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - High Correlation of the Response of Upper and Lower Lobe Small Airway Epithelium to Smoking

AU - Harvey, Ben Gary

AU - Strulovici-Barel, Yael

AU - Vincent, Thomas L.

AU - Mezey, Jason G.

AU - Raviram, Ramya

AU - Gordon, Cynthia

AU - Salit, Jacqueline

AU - Tilley, Ann E.

AU - Chung, Augustine

AU - Sanders, Abraham

AU - Crystal, Ronald

PY - 2013/9/9

Y1 - 2013/9/9

N2 - The distribution of lung disease induced by inhaled cigarette smoke is complex, depending on many factors. With the knowledge that the small airway epithelium (SAE) is the earliest site of smoking-induced lung disease, and that the SAE gene expression is likely sensitive to inhaled cigarette smoke, we compared upper vs. lower lobe gene expression in the SAE within the same cigarette smokers to determine if the gene expression patterns were similar or different. Active smokers (n = 11) with early evidence of smoking-induced lung disease (normal spirometry but low diffusing capacity) underwent bronchoscopy and brushing of the upper and lower lobe SAE in order to compare upper vs lower lobe genome-wide and smoking-responsive gene expression by microarray. Cluster and principal component analysis demonstrated that, for each individual, the expression of the known SAE smoking-responsive genes were highly correlated in upper and lower lobe pairs, although, as expected, there were differences in the smoking-induced changes in gene expression from individual to individual. These observations support the concept that the heterogeneity observed among smokers in the anatomic distribution of smoking-induced disease are not secondary to the topographic differences in the effects of cigarette smoke on the airway epithelium.

AB - The distribution of lung disease induced by inhaled cigarette smoke is complex, depending on many factors. With the knowledge that the small airway epithelium (SAE) is the earliest site of smoking-induced lung disease, and that the SAE gene expression is likely sensitive to inhaled cigarette smoke, we compared upper vs. lower lobe gene expression in the SAE within the same cigarette smokers to determine if the gene expression patterns were similar or different. Active smokers (n = 11) with early evidence of smoking-induced lung disease (normal spirometry but low diffusing capacity) underwent bronchoscopy and brushing of the upper and lower lobe SAE in order to compare upper vs lower lobe genome-wide and smoking-responsive gene expression by microarray. Cluster and principal component analysis demonstrated that, for each individual, the expression of the known SAE smoking-responsive genes were highly correlated in upper and lower lobe pairs, although, as expected, there were differences in the smoking-induced changes in gene expression from individual to individual. These observations support the concept that the heterogeneity observed among smokers in the anatomic distribution of smoking-induced disease are not secondary to the topographic differences in the effects of cigarette smoke on the airway epithelium.

UR - http://www.scopus.com/inward/record.url?scp=84883637161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883637161&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0072669

DO - 10.1371/journal.pone.0072669

M3 - Article

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e72669

ER -