HCV RNA levels in a multiethnic cohort of injection drug users: Human genetic, viral and demographic associations

Lorenzo Uccellini, Fan Chen Tseng, Alessandro Monaco, Fatma M. Shebl, Ruth Pfeiffer, Myhanh Dotrang, Dianna Buckett, Michael P. Busch, Ena Wang, Brian R. Edlin, Francesco M. Marincola, Thomas R. O'Brien

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In patients with chronic hepatitis C, the hepatitis C virus (HCV) RNA level is an important predictor of treatment response. To explore the relationship of HCV RNA with viral and demographic factors, as well as IL28B genotype, we examined viral levels in an ethnically diverse group of injection drug users (IDUs). Between 1998 and 2000, the Urban Health Study (UHS) recruited IDUs from street settings in San Francisco Bay area neighborhoods. Participants who were positive by HCV enzyme immunoassay were tested for HCV viremia by a branched-chain DNA assay. HCV genotype was determined by sequencing the HCV nonstructural 5B protein region. For a subset of participants, IL28B rs12979860 genotype was determined by Taqman. Among 1,701 participants with HCV viremia, median age was 46 years and median duration of injection drug use was 26 years; 56.0% were African American and 34.0% were of European ancestry (non-Hispanic). Human immunodeficiency virus type 1 (HIV-1) prevalence was 13.9%. The overall median HCV RNA level was 6.45 log 10 copies/mL. In unadjusted analyses, higher levels were found with older age, male gender, African-American ancestry, hepatitis B virus infection, HIV-1 infection, and IL28B rs12979860-CC genotype; compared to participants infected with HCV genotype 1, HCV RNA was lower in participants with genotypes 3 or 4. In an adjusted analysis, age, gender, racial ancestry, HIV-1 infection, HCV genotype, and IL28B rs12979860 genotype were all independently associated with HCV RNA. Conclusion: The level of HCV viremia is influenced by a large number of demographic, viral, and human genetic factors.

Original languageEnglish
Pages (from-to)86-94
Number of pages9
JournalHepatology
Volume56
Issue number1
DOIs
Publication statusPublished - Jul 2012
Externally publishedYes

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Medical Genetics
Drug Users
Hepacivirus
Demography
RNA
Injections
Genotype
Viremia
Virus Diseases
HIV-1
African Americans
Branched DNA Signal Amplification Assay
Urban Health
San Francisco
Viral RNA
Chronic Hepatitis C
Immunoenzyme Techniques
Hepatitis B virus

ASJC Scopus subject areas

  • Medicine(all)
  • Hepatology

Cite this

Uccellini, L., Tseng, F. C., Monaco, A., Shebl, F. M., Pfeiffer, R., Dotrang, M., ... O'Brien, T. R. (2012). HCV RNA levels in a multiethnic cohort of injection drug users: Human genetic, viral and demographic associations. Hepatology, 56(1), 86-94. https://doi.org/10.1002/hep.25652

HCV RNA levels in a multiethnic cohort of injection drug users : Human genetic, viral and demographic associations. / Uccellini, Lorenzo; Tseng, Fan Chen; Monaco, Alessandro; Shebl, Fatma M.; Pfeiffer, Ruth; Dotrang, Myhanh; Buckett, Dianna; Busch, Michael P.; Wang, Ena; Edlin, Brian R.; Marincola, Francesco M.; O'Brien, Thomas R.

In: Hepatology, Vol. 56, No. 1, 07.2012, p. 86-94.

Research output: Contribution to journalArticle

Uccellini, L, Tseng, FC, Monaco, A, Shebl, FM, Pfeiffer, R, Dotrang, M, Buckett, D, Busch, MP, Wang, E, Edlin, BR, Marincola, FM & O'Brien, TR 2012, 'HCV RNA levels in a multiethnic cohort of injection drug users: Human genetic, viral and demographic associations', Hepatology, vol. 56, no. 1, pp. 86-94. https://doi.org/10.1002/hep.25652
Uccellini, Lorenzo ; Tseng, Fan Chen ; Monaco, Alessandro ; Shebl, Fatma M. ; Pfeiffer, Ruth ; Dotrang, Myhanh ; Buckett, Dianna ; Busch, Michael P. ; Wang, Ena ; Edlin, Brian R. ; Marincola, Francesco M. ; O'Brien, Thomas R. / HCV RNA levels in a multiethnic cohort of injection drug users : Human genetic, viral and demographic associations. In: Hepatology. 2012 ; Vol. 56, No. 1. pp. 86-94.
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abstract = "In patients with chronic hepatitis C, the hepatitis C virus (HCV) RNA level is an important predictor of treatment response. To explore the relationship of HCV RNA with viral and demographic factors, as well as IL28B genotype, we examined viral levels in an ethnically diverse group of injection drug users (IDUs). Between 1998 and 2000, the Urban Health Study (UHS) recruited IDUs from street settings in San Francisco Bay area neighborhoods. Participants who were positive by HCV enzyme immunoassay were tested for HCV viremia by a branched-chain DNA assay. HCV genotype was determined by sequencing the HCV nonstructural 5B protein region. For a subset of participants, IL28B rs12979860 genotype was determined by Taqman. Among 1,701 participants with HCV viremia, median age was 46 years and median duration of injection drug use was 26 years; 56.0{\%} were African American and 34.0{\%} were of European ancestry (non-Hispanic). Human immunodeficiency virus type 1 (HIV-1) prevalence was 13.9{\%}. The overall median HCV RNA level was 6.45 log 10 copies/mL. In unadjusted analyses, higher levels were found with older age, male gender, African-American ancestry, hepatitis B virus infection, HIV-1 infection, and IL28B rs12979860-CC genotype; compared to participants infected with HCV genotype 1, HCV RNA was lower in participants with genotypes 3 or 4. In an adjusted analysis, age, gender, racial ancestry, HIV-1 infection, HCV genotype, and IL28B rs12979860 genotype were all independently associated with HCV RNA. Conclusion: The level of HCV viremia is influenced by a large number of demographic, viral, and human genetic factors.",
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