Gut uropathogen abundance is a risk factor for development of bacteriuria and urinary tract infection

Matthew Magruder, Adam N. Sholi, Catherine Gong, Lisa Zhang, Emmanuel Edusei, Jennifer Huang, Shady Albakry, Michael J. Satlin, Lars F. Westblade, Carl Crawford, Darshana M. Dadhania, Michelle Lubetzky, Ying Taur, Eric Littman, Lilan Ling, Philip Burnham, Iwijn De Vlaminck, Eric Pamer, Manikkam Suthanthiran, John Richard Lee

Research output: Contribution to journalArticle

Abstract

The origin of most bacterial infections in the urinary tract is often presumed to be the gut. Herein, we investigate the relationship between the gut microbiota and future development of bacteriuria and urinary tract infection (UTI). We perform gut microbial profiling using 16S rRNA gene deep sequencing on 510 fecal specimens from 168 kidney transplant recipients and metagenomic sequencing on a subset of fecal specimens and urine supernatant specimens. We report that a 1% relative gut abundance of Escherichia is an independent risk factor for Escherichia bacteriuria and UTI and a 1% relative gut abundance of Enterococcus is an independent risk factor for Enterococcus bacteriuria. Strain analysis establishes a close strain level alignment between species found in the gut and in the urine in the same subjects. Our results support a gut microbiota–UTI axis, suggesting that modulating the gut microbiota may be a potential novel strategy to prevent UTIs.

Original languageEnglish
Article number5521
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

Fingerprint

Bacteriuria
infectious diseases
Urinary Tract Infections
Escherichia
sequencing
urine
Enterococcus
Transplants
Urine
High-Throughput Nucleotide Sequencing
Metagenomics
Genes
kidneys
Urinary Tract
rRNA Genes
Bacterial Infections
genes
set theory
alignment
Kidney

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Magruder, M., Sholi, A. N., Gong, C., Zhang, L., Edusei, E., Huang, J., ... Lee, J. R. (2019). Gut uropathogen abundance is a risk factor for development of bacteriuria and urinary tract infection. Nature communications, 10(1), [5521]. https://doi.org/10.1038/s41467-019-13467-w

Gut uropathogen abundance is a risk factor for development of bacteriuria and urinary tract infection. / Magruder, Matthew; Sholi, Adam N.; Gong, Catherine; Zhang, Lisa; Edusei, Emmanuel; Huang, Jennifer; Albakry, Shady; Satlin, Michael J.; Westblade, Lars F.; Crawford, Carl; Dadhania, Darshana M.; Lubetzky, Michelle; Taur, Ying; Littman, Eric; Ling, Lilan; Burnham, Philip; De Vlaminck, Iwijn; Pamer, Eric; Suthanthiran, Manikkam; Lee, John Richard.

In: Nature communications, Vol. 10, No. 1, 5521, 01.12.2019.

Research output: Contribution to journalArticle

Magruder, M, Sholi, AN, Gong, C, Zhang, L, Edusei, E, Huang, J, Albakry, S, Satlin, MJ, Westblade, LF, Crawford, C, Dadhania, DM, Lubetzky, M, Taur, Y, Littman, E, Ling, L, Burnham, P, De Vlaminck, I, Pamer, E, Suthanthiran, M & Lee, JR 2019, 'Gut uropathogen abundance is a risk factor for development of bacteriuria and urinary tract infection', Nature communications, vol. 10, no. 1, 5521. https://doi.org/10.1038/s41467-019-13467-w
Magruder, Matthew ; Sholi, Adam N. ; Gong, Catherine ; Zhang, Lisa ; Edusei, Emmanuel ; Huang, Jennifer ; Albakry, Shady ; Satlin, Michael J. ; Westblade, Lars F. ; Crawford, Carl ; Dadhania, Darshana M. ; Lubetzky, Michelle ; Taur, Ying ; Littman, Eric ; Ling, Lilan ; Burnham, Philip ; De Vlaminck, Iwijn ; Pamer, Eric ; Suthanthiran, Manikkam ; Lee, John Richard. / Gut uropathogen abundance is a risk factor for development of bacteriuria and urinary tract infection. In: Nature communications. 2019 ; Vol. 10, No. 1.
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