Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli

Mahbuba Rahman, Mohammad Rubayet Hasan, Kazuyuki Shimizu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (∼1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.

Original languageEnglish
Pages (from-to)853-860
Number of pages8
JournalBiotechnology Letters
Volume30
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Regulator Genes
Metabolic Networks and Pathways
Escherichia coli
Genes
Growth
Batch Cell Culture Techniques

Keywords

  • Batch fermentation
  • Escherichia coli
  • Global regulators
  • Metabolic pathways
  • rpoS

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli. / Rahman, Mahbuba; Hasan, Mohammad Rubayet; Shimizu, Kazuyuki.

In: Biotechnology Letters, Vol. 30, No. 5, 05.2008, p. 853-860.

Research output: Contribution to journalArticle

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