Glycosylphosphatidylinositol‐linked Db does not induce an influenza‐specific cytotoxic T lymphocyte response or recycle membrane‐bound peptides

Ussama M. Abdel-Motal, Charles L. Sentman, Xianzheng Zhou, Peter J. Robinson, Jan Dahmén, Mikael Jondal

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Major histocompatibility complex (MHC) class I molecules, as well as MHC class I‐bound peptides, are known to recycle between the cell surface and an undefined, endosomal‐like compartment. Little is known about the functional significance of this process. We have explored this using two different forms of the H‐2Db molecule expressed in transgenic mice, either transmembranous (Db‐tm) or with a glycophosphatidylinositol (GPI)‐lipid anchor (Db‐GPI). The recycling capacity of peptides bound to Db‐tm and Db‐GPI was investigated using glycosylated Db‐binding glycopeptides, which were detected by flow cytometry. Only the tm form of Db was found to readily internalize and recycle glycopeptides to the cell surface. When transgenic mice were immunized with influenza A virus (PR8) strain and tested for cytotoxic T lymphocyte (CTL) responses against an immunedominant nucleoprotein epitope (366–374, ASNENMETM), onyl Db‐tm mice were found to generate specific CTL responses. The results support the idea that membrane recycling of MHC class I‐bound peptides on antigen‐presenting cells may be important for the generation of certain CTL responses.

Original languageEnglish
Pages (from-to)1121-1124
Number of pages4
JournalEuropean Journal of Immunology
Volume25
Issue number4
DOIs
Publication statusPublished - 1995
Externally publishedYes

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Keywords

  • Cytotoxic T lymphocyte
  • Major histocompatibility complex class I
  • Peptide
  • Recycling

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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