Glucose restriction decreases telomerase activity and enhances its inhibitor response on breast cancer cells

Possible extra-telomerase role of BIBR 1532

Layal Wardi, Nada Alaaeddine, Issam Raad, Riad Sarkis, Rim Serhal, Charbel Abi Khalil, George Hilal

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Considerable progress has been made to understand the association between lifestyle and diet in cancer initiation and promotion. Because excessive glucose consumption is a key metabolic hallmark of cancer cells, glucose restriction (GR) decreases the proliferation, and promotes the differentiation and transformation of cancer cells to quiescent cells. The immortality of cancerous cells is largely assured by telomerase, which is an interesting target for inhibition by BIBR 1532. In this study, we investigated the effect of GR on telomerase activity and on the efficacy of its inhibition by BIBR 1532.Methods: Breast cancer MDA-MB 231 and MCF-7 cells were cultured in DMEM (Dulbecco's modified eagle's media) with 0, 1 or 4.5 g/l of glucose. The telomerase activity was measured via quantitative Real-Time PCR, and the two telomerase subunits were semi-quantified by RT-PCR. Proliferation test and mitochondrial metabolism were assessed via tetrazolium salt reduction and cell counts; apoptosis was assessed via caspase-3 quantification and flow cytometry. Results: A decrease in the telomerase activity of more than 75% was associated with a significant reduction in the mRNA expression of its catalytic subunit hTERT (Reverse Transcriptase) and a decrease in the mitochondrial metabolism by more than 80% under restricted glucose conditions. In addition, GR increased the effect of BIBR 1532. Glucose deprivation induces apoptosis via BIBR 1532-mediated telomerase inhibition in triple negative breast cancer cells, as assessed by caspase-3 measurements and Annexin analysis.Conclusions: Taken together, our results suggest that the effect of BIBR 1532 is potentiated by GR to induce triple negative breast cancer cell death.

Original languageEnglish
Article number60
JournalCancer Cell International
Volume14
Issue number1
DOIs
Publication statusPublished - 4 Jul 2014
Externally publishedYes

Fingerprint

Telomerase
Breast Neoplasms
Glucose
Triple Negative Breast Neoplasms
Caspase 3
Tetrazolium Salts
Apoptosis
Annexins
Neoplasms
Eagles
BIBR 1532
RNA-Directed DNA Polymerase
MCF-7 Cells
Life Style
Real-Time Polymerase Chain Reaction
Catalytic Domain
Flow Cytometry
Cell Death
Cell Count
Diet

Keywords

  • BIBR 1532
  • Breast cancer
  • Cancer
  • Glucose restriction
  • Telomerase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Genetics

Cite this

Glucose restriction decreases telomerase activity and enhances its inhibitor response on breast cancer cells : Possible extra-telomerase role of BIBR 1532. / Wardi, Layal; Alaaeddine, Nada; Raad, Issam; Sarkis, Riad; Serhal, Rim; Abi Khalil, Charbel; Hilal, George.

In: Cancer Cell International, Vol. 14, No. 1, 60, 04.07.2014.

Research output: Contribution to journalArticle

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abstract = "Background: Considerable progress has been made to understand the association between lifestyle and diet in cancer initiation and promotion. Because excessive glucose consumption is a key metabolic hallmark of cancer cells, glucose restriction (GR) decreases the proliferation, and promotes the differentiation and transformation of cancer cells to quiescent cells. The immortality of cancerous cells is largely assured by telomerase, which is an interesting target for inhibition by BIBR 1532. In this study, we investigated the effect of GR on telomerase activity and on the efficacy of its inhibition by BIBR 1532.Methods: Breast cancer MDA-MB 231 and MCF-7 cells were cultured in DMEM (Dulbecco's modified eagle's media) with 0, 1 or 4.5 g/l of glucose. The telomerase activity was measured via quantitative Real-Time PCR, and the two telomerase subunits were semi-quantified by RT-PCR. Proliferation test and mitochondrial metabolism were assessed via tetrazolium salt reduction and cell counts; apoptosis was assessed via caspase-3 quantification and flow cytometry. Results: A decrease in the telomerase activity of more than 75{\%} was associated with a significant reduction in the mRNA expression of its catalytic subunit hTERT (Reverse Transcriptase) and a decrease in the mitochondrial metabolism by more than 80{\%} under restricted glucose conditions. In addition, GR increased the effect of BIBR 1532. Glucose deprivation induces apoptosis via BIBR 1532-mediated telomerase inhibition in triple negative breast cancer cells, as assessed by caspase-3 measurements and Annexin analysis.Conclusions: Taken together, our results suggest that the effect of BIBR 1532 is potentiated by GR to induce triple negative breast cancer cell death.",
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