Glucose 6-phosphate accumulation in mycobacteria implications for a novel f420-dependent anti-oxidant defense system

Mohammad Rubayet Hasan, Mahbuba Rahman, Sandford Jaques, Endang Purwantini, Lacy Daniels

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Glucose 6-phosphate (G6P) is a metabolic intermediate with many possible cellular fates. In mycobacteria, G6P is a substrate for an enzyme, F 420-dependent glucose-6-phosphate dehydrogenase (Fgd), found in few bacterial genera. Intracellular G6P levels in six Mycobacterium sp. were remarkably higher (∼17-130-fold) than Escherichia coli and Bacillus megaterium. The high G6P level in Mycobacterium smegmatis may result from 10-25-fold higher activity of the gluconeogenic enzyme fructose-1,6- bisphosphatase when grown on glucose, glycerol, or acetate compared with B. megaterium and E. coli. In M. smegmatis this coincided with up-regulation of the first gluconeogenic enzyme, phosphoenolpyruvate carboxykinase, when acetate was the carbon source, suggesting a cellular program for maintaining high G6P levels. G6P was depleted in cells under oxidative stress induced by redox cycling agents plumbagin and menadione, whereas an fgd mutant of M. smegmatis used G6P less well under such conditions. The fgd mutant was more sensitive to these agents and, in contrast to wild type, was defective in its ability to reduce extracellular plumbagin and menadione. These data suggest that intracellular G6P in mycobacteria serves as a source of reducing power and, with the mycobacteria-specific Fgd-F420 system, plays a protective role against oxidative stress.

Original languageEnglish
Pages (from-to)19135-19144
Number of pages10
JournalJournal of Biological Chemistry
Volume285
Issue number25
DOIs
Publication statusPublished - 18 Jun 2010
Externally publishedYes

Fingerprint

Glucose-6-Phosphate
Mycobacterium
Oxidants
Mycobacterium smegmatis
Bacillus megaterium
Vitamin K 3
Oxidative stress
Escherichia coli
Acetates
Oxidative Stress
Enzymes
Fructose-Bisphosphatase
Electric Power Supplies
Phosphoenolpyruvate
Glucosephosphate Dehydrogenase
Bacilli
Glycerol
Contrast Media
Oxidation-Reduction
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Glucose 6-phosphate accumulation in mycobacteria implications for a novel f420-dependent anti-oxidant defense system. / Hasan, Mohammad Rubayet; Rahman, Mahbuba; Jaques, Sandford; Purwantini, Endang; Daniels, Lacy.

In: Journal of Biological Chemistry, Vol. 285, No. 25, 18.06.2010, p. 19135-19144.

Research output: Contribution to journalArticle

Hasan, Mohammad Rubayet ; Rahman, Mahbuba ; Jaques, Sandford ; Purwantini, Endang ; Daniels, Lacy. / Glucose 6-phosphate accumulation in mycobacteria implications for a novel f420-dependent anti-oxidant defense system. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 25. pp. 19135-19144.
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