Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions

SooA Lim, Esteban Marcellin, Shana Jacob, Lars K. Nielsen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Little is known about the role of global phosphorylation events in the control of prokaryote metabolism. By performing a detailed analysis of all protein phosphorylation events previously reported in Escherichia coli, dynamic changes in protein phosphorylation were elucidated under three different culture conditions. Using scheduled reaction monitoring, the phosphorylation ratios of 82 peptides corresponding to 71 proteins were quantified to establish whether serine (S), threonine (T) and tyrosine (Y) phosphorylation events displayed a dynamic profile under changing culture conditions. The ratio of phosphorylation for 23 enzymes from central carbon metabolism was found to be dynamic. The data presented contributes to our understanding of the global role of phosphorylation in bacterial metabolism and highlight that phosphorylation is an important, yet poorly understood, regulatory mechanism of metabolism control in bacteria.

Original languageEnglish
Pages (from-to)24-33
Number of pages10
JournalJournal of Proteomics
Volume126
DOIs
Publication statusPublished - 3 Aug 2015
Externally publishedYes

Fingerprint

Phosphorylation
Metabolism
Escherichia coli
Carbon
Proteins
Threonine
Serine
Tyrosine
Bacteria
Peptides
Monitoring
Enzymes

Keywords

  • Central carbon metabolism
  • Phosphoproteomics
  • Phosphorylation
  • Quantitative phosphoproteomics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions. / Lim, SooA; Marcellin, Esteban; Jacob, Shana; Nielsen, Lars K.

In: Journal of Proteomics, Vol. 126, 03.08.2015, p. 24-33.

Research output: Contribution to journalArticle

Lim, SooA ; Marcellin, Esteban ; Jacob, Shana ; Nielsen, Lars K. / Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions. In: Journal of Proteomics. 2015 ; Vol. 126. pp. 24-33.
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