Glibenclamide impairs responses of neutrophils against Burkholderia pseudomallei by reduction of intracellular glutathione

Chidchamai Kewcharoenwong, Darawan Rinchai, Arnone Nithichanon, Gregory J. Bancroft, Manabu Ato, Ganjana Lertmemongkolchai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The major risk factor for melioidosis, an infectious disease caused by B. pseudomallei, is diabetes mellitus. More than half of diabetic melioidosis patients in Thailand were prescribed glibenclamide. Recent evidence demonstrates that glibenclamide reduces pro-inflammatory cytokine production by polymorphonuclear neutrophils (PMNs) of diabetic individuals in response to this bacterial infection. However, the mechanisms by which glibenclamide affects cytokine production are unknown. We found that PMNs from glibenclamide-treated diabetic individuals infected with live B. pseudomallei in vitro showed lower free glutathione (GSH) levels compared with those of healthy individuals. Glibenclamide decreased GSH levels and glutathione peroxidase (GPx) of PMNs after exposed to live B. pseudomallei. Moreover, glibenclamide reduced cytokine production and migration capacity of infected PMNs, whereas GSH could restore these functions. Taken together, our data show a link between the effect of glibenclamide on GSH and PMN functions in response to B. pseudomallei that may contribute to the susceptibility of diabetic individuals to B. pseudomallei infection.

Original languageEnglish
Article number34794
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 7 Oct 2016
Externally publishedYes

Fingerprint

Burkholderia pseudomallei
Glyburide
Glutathione
Neutrophils
Melioidosis
Cytokines
Burkholderia Infections
Thailand
Glutathione Peroxidase
Bacterial Infections
Communicable Diseases
Diabetes Mellitus

ASJC Scopus subject areas

  • General

Cite this

Glibenclamide impairs responses of neutrophils against Burkholderia pseudomallei by reduction of intracellular glutathione. / Kewcharoenwong, Chidchamai; Rinchai, Darawan; Nithichanon, Arnone; Bancroft, Gregory J.; Ato, Manabu; Lertmemongkolchai, Ganjana.

In: Scientific Reports, Vol. 6, 34794, 07.10.2016.

Research output: Contribution to journalArticle

Kewcharoenwong, Chidchamai ; Rinchai, Darawan ; Nithichanon, Arnone ; Bancroft, Gregory J. ; Ato, Manabu ; Lertmemongkolchai, Ganjana. / Glibenclamide impairs responses of neutrophils against Burkholderia pseudomallei by reduction of intracellular glutathione. In: Scientific Reports. 2016 ; Vol. 6.
@article{607e8ff9d97a4f56a7a836d4b9729dce,
title = "Glibenclamide impairs responses of neutrophils against Burkholderia pseudomallei by reduction of intracellular glutathione",
abstract = "The major risk factor for melioidosis, an infectious disease caused by B. pseudomallei, is diabetes mellitus. More than half of diabetic melioidosis patients in Thailand were prescribed glibenclamide. Recent evidence demonstrates that glibenclamide reduces pro-inflammatory cytokine production by polymorphonuclear neutrophils (PMNs) of diabetic individuals in response to this bacterial infection. However, the mechanisms by which glibenclamide affects cytokine production are unknown. We found that PMNs from glibenclamide-treated diabetic individuals infected with live B. pseudomallei in vitro showed lower free glutathione (GSH) levels compared with those of healthy individuals. Glibenclamide decreased GSH levels and glutathione peroxidase (GPx) of PMNs after exposed to live B. pseudomallei. Moreover, glibenclamide reduced cytokine production and migration capacity of infected PMNs, whereas GSH could restore these functions. Taken together, our data show a link between the effect of glibenclamide on GSH and PMN functions in response to B. pseudomallei that may contribute to the susceptibility of diabetic individuals to B. pseudomallei infection.",
author = "Chidchamai Kewcharoenwong and Darawan Rinchai and Arnone Nithichanon and Bancroft, {Gregory J.} and Manabu Ato and Ganjana Lertmemongkolchai",
year = "2016",
month = "10",
day = "7",
doi = "10.1038/srep34794",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Glibenclamide impairs responses of neutrophils against Burkholderia pseudomallei by reduction of intracellular glutathione

AU - Kewcharoenwong, Chidchamai

AU - Rinchai, Darawan

AU - Nithichanon, Arnone

AU - Bancroft, Gregory J.

AU - Ato, Manabu

AU - Lertmemongkolchai, Ganjana

PY - 2016/10/7

Y1 - 2016/10/7

N2 - The major risk factor for melioidosis, an infectious disease caused by B. pseudomallei, is diabetes mellitus. More than half of diabetic melioidosis patients in Thailand were prescribed glibenclamide. Recent evidence demonstrates that glibenclamide reduces pro-inflammatory cytokine production by polymorphonuclear neutrophils (PMNs) of diabetic individuals in response to this bacterial infection. However, the mechanisms by which glibenclamide affects cytokine production are unknown. We found that PMNs from glibenclamide-treated diabetic individuals infected with live B. pseudomallei in vitro showed lower free glutathione (GSH) levels compared with those of healthy individuals. Glibenclamide decreased GSH levels and glutathione peroxidase (GPx) of PMNs after exposed to live B. pseudomallei. Moreover, glibenclamide reduced cytokine production and migration capacity of infected PMNs, whereas GSH could restore these functions. Taken together, our data show a link between the effect of glibenclamide on GSH and PMN functions in response to B. pseudomallei that may contribute to the susceptibility of diabetic individuals to B. pseudomallei infection.

AB - The major risk factor for melioidosis, an infectious disease caused by B. pseudomallei, is diabetes mellitus. More than half of diabetic melioidosis patients in Thailand were prescribed glibenclamide. Recent evidence demonstrates that glibenclamide reduces pro-inflammatory cytokine production by polymorphonuclear neutrophils (PMNs) of diabetic individuals in response to this bacterial infection. However, the mechanisms by which glibenclamide affects cytokine production are unknown. We found that PMNs from glibenclamide-treated diabetic individuals infected with live B. pseudomallei in vitro showed lower free glutathione (GSH) levels compared with those of healthy individuals. Glibenclamide decreased GSH levels and glutathione peroxidase (GPx) of PMNs after exposed to live B. pseudomallei. Moreover, glibenclamide reduced cytokine production and migration capacity of infected PMNs, whereas GSH could restore these functions. Taken together, our data show a link between the effect of glibenclamide on GSH and PMN functions in response to B. pseudomallei that may contribute to the susceptibility of diabetic individuals to B. pseudomallei infection.

UR - http://www.scopus.com/inward/record.url?scp=84991073703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991073703&partnerID=8YFLogxK

U2 - 10.1038/srep34794

DO - 10.1038/srep34794

M3 - Article

C2 - 27713554

AN - SCOPUS:84991073703

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 34794

ER -