Genus-wide sequencing supports a two-locus model for sex-determination in Phoenix

Maria F. Torres, Lisa Sara Mathew, Ikhlak Ahmed, Iman Al Azwani, Robert Krueger, Diego Rivera-Nuñez, Yasmin Ali Mohamoud, Andrew G. Clark, Karsten Suhre, Joel Malek

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The date palm tree is a commercially important member of the genus Phoenix whose 14 species are dioecious with separate male and female individuals. To identify sex determining genes we sequenced the genomes of 15 female and 13 male Phoenix trees representing all 14 species. We identified male-specific sequences and extended them using phased single-molecule sequencing or BAC clones. We observed that only four genes contained sequences conserved in all analyzed Phoenix males. Most of these sequences showed similarity to a single genomic locus in the closely related monoecious oil palm. CYP703 and GPAT3, two single copy genes present in males and critical for male flower development in other monocots, were absent in females. A LOG-like gene appears translocated into the Y-linked region and is suggested to play a role in suppressing female flowers. Our data are consistent with a two-mutation model for the evolution of dioecy in Phoenix.

Original languageEnglish
Article number3969
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Phoenix (AZ)
sequencing
loci
Genes
genes
Palm oil
Conserved Sequence
genome
mutations
Oils
Clone Cells
oils
Molecules
Genome
Mutation
molecules

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Genus-wide sequencing supports a two-locus model for sex-determination in Phoenix. / Torres, Maria F.; Mathew, Lisa Sara; Ahmed, Ikhlak; Al Azwani, Iman; Krueger, Robert; Rivera-Nuñez, Diego; Ali Mohamoud, Yasmin; Clark, Andrew G.; Suhre, Karsten; Malek, Joel.

In: Nature Communications, Vol. 9, No. 1, 3969, 01.12.2018.

Research output: Contribution to journalArticle

@article{e94f8f083fcc495b836f7aabde58276d,
title = "Genus-wide sequencing supports a two-locus model for sex-determination in Phoenix",
abstract = "The date palm tree is a commercially important member of the genus Phoenix whose 14 species are dioecious with separate male and female individuals. To identify sex determining genes we sequenced the genomes of 15 female and 13 male Phoenix trees representing all 14 species. We identified male-specific sequences and extended them using phased single-molecule sequencing or BAC clones. We observed that only four genes contained sequences conserved in all analyzed Phoenix males. Most of these sequences showed similarity to a single genomic locus in the closely related monoecious oil palm. CYP703 and GPAT3, two single copy genes present in males and critical for male flower development in other monocots, were absent in females. A LOG-like gene appears translocated into the Y-linked region and is suggested to play a role in suppressing female flowers. Our data are consistent with a two-mutation model for the evolution of dioecy in Phoenix.",
author = "Torres, {Maria F.} and Mathew, {Lisa Sara} and Ikhlak Ahmed and {Al Azwani}, Iman and Robert Krueger and Diego Rivera-Nu{\~n}ez and {Ali Mohamoud}, Yasmin and Clark, {Andrew G.} and Karsten Suhre and Joel Malek",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41467-018-06375-y",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Genus-wide sequencing supports a two-locus model for sex-determination in Phoenix

AU - Torres, Maria F.

AU - Mathew, Lisa Sara

AU - Ahmed, Ikhlak

AU - Al Azwani, Iman

AU - Krueger, Robert

AU - Rivera-Nuñez, Diego

AU - Ali Mohamoud, Yasmin

AU - Clark, Andrew G.

AU - Suhre, Karsten

AU - Malek, Joel

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The date palm tree is a commercially important member of the genus Phoenix whose 14 species are dioecious with separate male and female individuals. To identify sex determining genes we sequenced the genomes of 15 female and 13 male Phoenix trees representing all 14 species. We identified male-specific sequences and extended them using phased single-molecule sequencing or BAC clones. We observed that only four genes contained sequences conserved in all analyzed Phoenix males. Most of these sequences showed similarity to a single genomic locus in the closely related monoecious oil palm. CYP703 and GPAT3, two single copy genes present in males and critical for male flower development in other monocots, were absent in females. A LOG-like gene appears translocated into the Y-linked region and is suggested to play a role in suppressing female flowers. Our data are consistent with a two-mutation model for the evolution of dioecy in Phoenix.

AB - The date palm tree is a commercially important member of the genus Phoenix whose 14 species are dioecious with separate male and female individuals. To identify sex determining genes we sequenced the genomes of 15 female and 13 male Phoenix trees representing all 14 species. We identified male-specific sequences and extended them using phased single-molecule sequencing or BAC clones. We observed that only four genes contained sequences conserved in all analyzed Phoenix males. Most of these sequences showed similarity to a single genomic locus in the closely related monoecious oil palm. CYP703 and GPAT3, two single copy genes present in males and critical for male flower development in other monocots, were absent in females. A LOG-like gene appears translocated into the Y-linked region and is suggested to play a role in suppressing female flowers. Our data are consistent with a two-mutation model for the evolution of dioecy in Phoenix.

UR - http://www.scopus.com/inward/record.url?scp=85054091321&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054091321&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-06375-y

DO - 10.1038/s41467-018-06375-y

M3 - Article

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3969

ER -