Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus

William C. Nierman, Arnab Pain, Michael J. Anderson, Jennifer R. Wortman, H. Stanley Kim, Javier Arroyo, Matthew Berriman, Keietsu Abe, David B. Archer, Clara Bermejo, Joan Bennett, Paul Bowyer, Dan Chen, Matthew Collins, Richard Coulsen, Robert Davies, Paul S. Dyer, Mark Farman, Nadia Fedorova, Natalie Fedorova & 78 others Tamara V. Feldblyum, Reinhard Fischer, Nigel Fosker, Audrey Fraser, Jose L. García, Maria J. García, Ariette Goble, Gustavo H. Goldman, Katsuya Gomi, Sam Griffith-Jones, Ryan Gwilliam, Brian Haas, Hubertus Haas, David Harris, H. Horiuchi, Jiaqi Huang, Sean Humphray, Javier Jiménez, Nancy Keller, Hoda Khouri, Katsuhiko Kitamoto, Tetsuo Kobayashi, Sven Konzack, Resham Kulkarni, Toshitaka Kumagai, Anne Lafton, Jean Paul Latgé, Weixi Li, Angela Lord, Charles Lu, William H. Majoros, Gregory S. May, Bruce L. Miller, Yasmin Ali Mohamoud, Maria Molina, Michel Monod, Isabelle Mouyna, Stephanie Mulligan, Lee Murphy, Susan O'Neil, Ian Paulsen, Miguel A. Peñalva, Mihaela Pertea, Claire Price, Bethan L. Pritchard, Michael A. Quail, Ester Rabbinowitsch, Neil Rawlins, Marie Adele Rajandream, Utz Reichard, Hubert Renauld, Geoffrey D. Robson, Santiago Rodriguez De Cordoba, Jose M. Rodríguez-Peña, Catherine M. Ronning, Simon Rutter, Steven L. Salzberg, Miguel Sanchez, Juan C. Sánchez-Ferrero, David Saunders, Kathy Seeger, Rob Squares, Steven Squares, Michio Takeuchi, Fredj Tekaia, Geoffrey Turner, Carlos R. Vazquez De Aldana, Janice Weidman, Owen White, John Woodward, Jae Hyuk Yu, Claire Fraser, James E. Galagan, Kiyoshi Asai, Masayuki Machida, Neil Hall, Bart Barrell, David W. Denning

Research output: Contribution to journalArticle

871 Citations (Scopus)

Abstract

Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen1-3. Its conidia production is prolific, and so human respiratory tract exposure is almost constant4. A. fumigatus is isolated from human habitats 5 and vegetable compost heaps6,7. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis8. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.

Original languageEnglish
Pages (from-to)1151-1156
Number of pages6
JournalNature
Volume438
Issue number7071
DOIs
Publication statusPublished - 22 Dec 2005
Externally publishedYes

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Aspergillus fumigatus
Fungi
Neosartorya
Genome
Genes
Chromosomes, Human, Pair 9
Fungal Spores
Microarray Analysis
Vegetables
Respiratory System
Ecosystem
Virulence
Immune System
Soil
Asthma
Phenotype
Temperature
Mortality
Incidence
Infection

ASJC Scopus subject areas

  • General

Cite this

Nierman, W. C., Pain, A., Anderson, M. J., Wortman, J. R., Kim, H. S., Arroyo, J., ... Denning, D. W. (2005). Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature, 438(7071), 1151-1156. https://doi.org/10.1038/nature04332

Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. / Nierman, William C.; Pain, Arnab; Anderson, Michael J.; Wortman, Jennifer R.; Kim, H. Stanley; Arroyo, Javier; Berriman, Matthew; Abe, Keietsu; Archer, David B.; Bermejo, Clara; Bennett, Joan; Bowyer, Paul; Chen, Dan; Collins, Matthew; Coulsen, Richard; Davies, Robert; Dyer, Paul S.; Farman, Mark; Fedorova, Nadia; Fedorova, Natalie; Feldblyum, Tamara V.; Fischer, Reinhard; Fosker, Nigel; Fraser, Audrey; García, Jose L.; García, Maria J.; Goble, Ariette; Goldman, Gustavo H.; Gomi, Katsuya; Griffith-Jones, Sam; Gwilliam, Ryan; Haas, Brian; Haas, Hubertus; Harris, David; Horiuchi, H.; Huang, Jiaqi; Humphray, Sean; Jiménez, Javier; Keller, Nancy; Khouri, Hoda; Kitamoto, Katsuhiko; Kobayashi, Tetsuo; Konzack, Sven; Kulkarni, Resham; Kumagai, Toshitaka; Lafton, Anne; Latgé, Jean Paul; Li, Weixi; Lord, Angela; Lu, Charles; Majoros, William H.; May, Gregory S.; Miller, Bruce L.; Ali Mohamoud, Yasmin; Molina, Maria; Monod, Michel; Mouyna, Isabelle; Mulligan, Stephanie; Murphy, Lee; O'Neil, Susan; Paulsen, Ian; Peñalva, Miguel A.; Pertea, Mihaela; Price, Claire; Pritchard, Bethan L.; Quail, Michael A.; Rabbinowitsch, Ester; Rawlins, Neil; Rajandream, Marie Adele; Reichard, Utz; Renauld, Hubert; Robson, Geoffrey D.; Rodriguez De Cordoba, Santiago; Rodríguez-Peña, Jose M.; Ronning, Catherine M.; Rutter, Simon; Salzberg, Steven L.; Sanchez, Miguel; Sánchez-Ferrero, Juan C.; Saunders, David; Seeger, Kathy; Squares, Rob; Squares, Steven; Takeuchi, Michio; Tekaia, Fredj; Turner, Geoffrey; Vazquez De Aldana, Carlos R.; Weidman, Janice; White, Owen; Woodward, John; Yu, Jae Hyuk; Fraser, Claire; Galagan, James E.; Asai, Kiyoshi; Machida, Masayuki; Hall, Neil; Barrell, Bart; Denning, David W.

In: Nature, Vol. 438, No. 7071, 22.12.2005, p. 1151-1156.

Research output: Contribution to journalArticle

Nierman, WC, Pain, A, Anderson, MJ, Wortman, JR, Kim, HS, Arroyo, J, Berriman, M, Abe, K, Archer, DB, Bermejo, C, Bennett, J, Bowyer, P, Chen, D, Collins, M, Coulsen, R, Davies, R, Dyer, PS, Farman, M, Fedorova, N, Fedorova, N, Feldblyum, TV, Fischer, R, Fosker, N, Fraser, A, García, JL, García, MJ, Goble, A, Goldman, GH, Gomi, K, Griffith-Jones, S, Gwilliam, R, Haas, B, Haas, H, Harris, D, Horiuchi, H, Huang, J, Humphray, S, Jiménez, J, Keller, N, Khouri, H, Kitamoto, K, Kobayashi, T, Konzack, S, Kulkarni, R, Kumagai, T, Lafton, A, Latgé, JP, Li, W, Lord, A, Lu, C, Majoros, WH, May, GS, Miller, BL, Ali Mohamoud, Y, Molina, M, Monod, M, Mouyna, I, Mulligan, S, Murphy, L, O'Neil, S, Paulsen, I, Peñalva, MA, Pertea, M, Price, C, Pritchard, BL, Quail, MA, Rabbinowitsch, E, Rawlins, N, Rajandream, MA, Reichard, U, Renauld, H, Robson, GD, Rodriguez De Cordoba, S, Rodríguez-Peña, JM, Ronning, CM, Rutter, S, Salzberg, SL, Sanchez, M, Sánchez-Ferrero, JC, Saunders, D, Seeger, K, Squares, R, Squares, S, Takeuchi, M, Tekaia, F, Turner, G, Vazquez De Aldana, CR, Weidman, J, White, O, Woodward, J, Yu, JH, Fraser, C, Galagan, JE, Asai, K, Machida, M, Hall, N, Barrell, B & Denning, DW 2005, 'Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus', Nature, vol. 438, no. 7071, pp. 1151-1156. https://doi.org/10.1038/nature04332
Nierman WC, Pain A, Anderson MJ, Wortman JR, Kim HS, Arroyo J et al. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature. 2005 Dec 22;438(7071):1151-1156. https://doi.org/10.1038/nature04332
Nierman, William C. ; Pain, Arnab ; Anderson, Michael J. ; Wortman, Jennifer R. ; Kim, H. Stanley ; Arroyo, Javier ; Berriman, Matthew ; Abe, Keietsu ; Archer, David B. ; Bermejo, Clara ; Bennett, Joan ; Bowyer, Paul ; Chen, Dan ; Collins, Matthew ; Coulsen, Richard ; Davies, Robert ; Dyer, Paul S. ; Farman, Mark ; Fedorova, Nadia ; Fedorova, Natalie ; Feldblyum, Tamara V. ; Fischer, Reinhard ; Fosker, Nigel ; Fraser, Audrey ; García, Jose L. ; García, Maria J. ; Goble, Ariette ; Goldman, Gustavo H. ; Gomi, Katsuya ; Griffith-Jones, Sam ; Gwilliam, Ryan ; Haas, Brian ; Haas, Hubertus ; Harris, David ; Horiuchi, H. ; Huang, Jiaqi ; Humphray, Sean ; Jiménez, Javier ; Keller, Nancy ; Khouri, Hoda ; Kitamoto, Katsuhiko ; Kobayashi, Tetsuo ; Konzack, Sven ; Kulkarni, Resham ; Kumagai, Toshitaka ; Lafton, Anne ; Latgé, Jean Paul ; Li, Weixi ; Lord, Angela ; Lu, Charles ; Majoros, William H. ; May, Gregory S. ; Miller, Bruce L. ; Ali Mohamoud, Yasmin ; Molina, Maria ; Monod, Michel ; Mouyna, Isabelle ; Mulligan, Stephanie ; Murphy, Lee ; O'Neil, Susan ; Paulsen, Ian ; Peñalva, Miguel A. ; Pertea, Mihaela ; Price, Claire ; Pritchard, Bethan L. ; Quail, Michael A. ; Rabbinowitsch, Ester ; Rawlins, Neil ; Rajandream, Marie Adele ; Reichard, Utz ; Renauld, Hubert ; Robson, Geoffrey D. ; Rodriguez De Cordoba, Santiago ; Rodríguez-Peña, Jose M. ; Ronning, Catherine M. ; Rutter, Simon ; Salzberg, Steven L. ; Sanchez, Miguel ; Sánchez-Ferrero, Juan C. ; Saunders, David ; Seeger, Kathy ; Squares, Rob ; Squares, Steven ; Takeuchi, Michio ; Tekaia, Fredj ; Turner, Geoffrey ; Vazquez De Aldana, Carlos R. ; Weidman, Janice ; White, Owen ; Woodward, John ; Yu, Jae Hyuk ; Fraser, Claire ; Galagan, James E. ; Asai, Kiyoshi ; Machida, Masayuki ; Hall, Neil ; Barrell, Bart ; Denning, David W. / Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. In: Nature. 2005 ; Vol. 438, No. 7071. pp. 1151-1156.
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abstract = "Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen1-3. Its conidia production is prolific, and so human respiratory tract exposure is almost constant4. A. fumigatus is isolated from human habitats 5 and vegetable compost heaps6,7. In immunocompromised individuals, the incidence of invasive infection can be as high as 50{\%} and the mortality rate is often about 50{\%} (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis8. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.",
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T1 - Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus

AU - Nierman, William C.

AU - Pain, Arnab

AU - Anderson, Michael J.

AU - Wortman, Jennifer R.

AU - Kim, H. Stanley

AU - Arroyo, Javier

AU - Berriman, Matthew

AU - Abe, Keietsu

AU - Archer, David B.

AU - Bermejo, Clara

AU - Bennett, Joan

AU - Bowyer, Paul

AU - Chen, Dan

AU - Collins, Matthew

AU - Coulsen, Richard

AU - Davies, Robert

AU - Dyer, Paul S.

AU - Farman, Mark

AU - Fedorova, Nadia

AU - Fedorova, Natalie

AU - Feldblyum, Tamara V.

AU - Fischer, Reinhard

AU - Fosker, Nigel

AU - Fraser, Audrey

AU - García, Jose L.

AU - García, Maria J.

AU - Goble, Ariette

AU - Goldman, Gustavo H.

AU - Gomi, Katsuya

AU - Griffith-Jones, Sam

AU - Gwilliam, Ryan

AU - Haas, Brian

AU - Haas, Hubertus

AU - Harris, David

AU - Horiuchi, H.

AU - Huang, Jiaqi

AU - Humphray, Sean

AU - Jiménez, Javier

AU - Keller, Nancy

AU - Khouri, Hoda

AU - Kitamoto, Katsuhiko

AU - Kobayashi, Tetsuo

AU - Konzack, Sven

AU - Kulkarni, Resham

AU - Kumagai, Toshitaka

AU - Lafton, Anne

AU - Latgé, Jean Paul

AU - Li, Weixi

AU - Lord, Angela

AU - Lu, Charles

AU - Majoros, William H.

AU - May, Gregory S.

AU - Miller, Bruce L.

AU - Ali Mohamoud, Yasmin

AU - Molina, Maria

AU - Monod, Michel

AU - Mouyna, Isabelle

AU - Mulligan, Stephanie

AU - Murphy, Lee

AU - O'Neil, Susan

AU - Paulsen, Ian

AU - Peñalva, Miguel A.

AU - Pertea, Mihaela

AU - Price, Claire

AU - Pritchard, Bethan L.

AU - Quail, Michael A.

AU - Rabbinowitsch, Ester

AU - Rawlins, Neil

AU - Rajandream, Marie Adele

AU - Reichard, Utz

AU - Renauld, Hubert

AU - Robson, Geoffrey D.

AU - Rodriguez De Cordoba, Santiago

AU - Rodríguez-Peña, Jose M.

AU - Ronning, Catherine M.

AU - Rutter, Simon

AU - Salzberg, Steven L.

AU - Sanchez, Miguel

AU - Sánchez-Ferrero, Juan C.

AU - Saunders, David

AU - Seeger, Kathy

AU - Squares, Rob

AU - Squares, Steven

AU - Takeuchi, Michio

AU - Tekaia, Fredj

AU - Turner, Geoffrey

AU - Vazquez De Aldana, Carlos R.

AU - Weidman, Janice

AU - White, Owen

AU - Woodward, John

AU - Yu, Jae Hyuk

AU - Fraser, Claire

AU - Galagan, James E.

AU - Asai, Kiyoshi

AU - Machida, Masayuki

AU - Hall, Neil

AU - Barrell, Bart

AU - Denning, David W.

PY - 2005/12/22

Y1 - 2005/12/22

N2 - Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen1-3. Its conidia production is prolific, and so human respiratory tract exposure is almost constant4. A. fumigatus is isolated from human habitats 5 and vegetable compost heaps6,7. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis8. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.

AB - Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen1-3. Its conidia production is prolific, and so human respiratory tract exposure is almost constant4. A. fumigatus is isolated from human habitats 5 and vegetable compost heaps6,7. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airborne fungi with the immune system is increasingly linked to severe asthma and sinusitis8. Although the burden of invasive disease caused by A. fumigatus is substantial, the basic biology of the organism is mostly obscure. Here we show the complete 29.4-megabase genome sequence of the clinical isolate Af293, which consists of eight chromosomes containing 9,926 predicted genes. Microarray analysis revealed temperature-dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype. The Af293 genome sequence provides an unparalleled resource for the future understanding of this remarkable fungus.

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