Genomic correlates of hyperthermostability, an update

Karsten Suhre, Jean Michel Claverie

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

It has been shown (Cambillau, C., and Claverie, J. M. (2000) J. Biol. Chem. 275, 32383-32386) that a large difference between the proportions of charged versus polar (non-charged) amino acids (CvP-bias) was an adequate, if empirical, signature of the proteome of hyperthermophilic organisms (Tgrowth > 80 °C). Since that study, the number of available microbial genomes has more than doubled, raising the possibility that the simple CvP-bias rule might no longer hold. Taking advantage of the new sequence data, we re-analyzed the genomes of 9 fully sequenced thermophiles, 9 hyperthermophiles, and 53 mesothermophile microorganisms to identify the genomic correlates of hyperthermostability on a wider data set. Our new results confirm that the CvP-bias previously identified on a much smaller data set still holds. Moreover, we show that it is an optimal criterion, in the sense that it corresponds to the most discriminating factor between hyperthermophilic and mesothermophilic microorganisms in a principal component analysis. In parallel, we evaluated two other recently proposed correlates of hyperthermostability, the proteome average pI and the dinucleotide statistical index (Kawashima, T., Amano, N., Koike, H., Makino, S., Higuchi, S., Kawashima-Ohya, Y., Watanabe, K., Yamazaki, M., Kanehori, K., Kawamoto, T., Nunoshiba, T., Yamamoto, Y., Aramaki, H., Makino, K., and Suzuki, M. (2000) Proc. Natl. Acad. Sci. 97, 14257-14262). We show that the CvP-bias is the sole criterion that is able to clearly discriminate hyperthermophile from mesothermophile microorganisms on a global genomic basis.

Original languageEnglish
Pages (from-to)17198-17202
Number of pages5
JournalJournal of Biological Chemistry
Volume278
Issue number19
DOIs
Publication statusPublished - 9 May 2003
Externally publishedYes

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Proteome
Microorganisms
Microbial Genome
Genes
Principal Component Analysis
Genome
Amino Acids
Principal component analysis
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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Genomic correlates of hyperthermostability, an update. / Suhre, Karsten; Claverie, Jean Michel.

In: Journal of Biological Chemistry, Vol. 278, No. 19, 09.05.2003, p. 17198-17202.

Research output: Contribution to journalArticle

Suhre, Karsten ; Claverie, Jean Michel. / Genomic correlates of hyperthermostability, an update. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 19. pp. 17198-17202.
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