Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

Garry S.A. Myers, Dane Parker, Keith Al-Hasani, Ruth M. Kennan, Torsten Seemann, Qinghu Ren, Jonathan H. Badger, Jeremy D. Selengut, Robert T. DeBoy, Hervé Tettelin, John D. Boyce, Victoria P. McCarl, Xiaoyan Han, William C. Nelson, Ramana Madupu, Yasmin Ali Mohamoud, Tara Holley, Nadia Fedorova, Hoda Khouri, Steven P. Bottomley & 5 others Richard J. Whittington, Ben Adler, J. Glenn Songer, Julian I. Rood, Ian T. Paulsen

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

Original languageEnglish
Pages (from-to)569-575
Number of pages7
JournalNature Biotechnology
Volume25
Issue number5
DOIs
Publication statusPublished - 1 May 2007
Externally publishedYes

Fingerprint

Dichelobacter nodosus
Vaccines
Pathogens
Antigens
Animals
Genes
Genome
Bioinformatics
Computational Biology
Proteins
Sheep
Gene transfer
Horizontal Gene Transfer
Meats
Wool
Virulence Factors
Microarrays
Carbohydrates
Meat
Virulence

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Myers, G. S. A., Parker, D., Al-Hasani, K., Kennan, R. M., Seemann, T., Ren, Q., ... Paulsen, I. T. (2007). Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus. Nature Biotechnology, 25(5), 569-575. https://doi.org/10.1038/nbt1302

Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus. / Myers, Garry S.A.; Parker, Dane; Al-Hasani, Keith; Kennan, Ruth M.; Seemann, Torsten; Ren, Qinghu; Badger, Jonathan H.; Selengut, Jeremy D.; DeBoy, Robert T.; Tettelin, Hervé; Boyce, John D.; McCarl, Victoria P.; Han, Xiaoyan; Nelson, William C.; Madupu, Ramana; Ali Mohamoud, Yasmin; Holley, Tara; Fedorova, Nadia; Khouri, Hoda; Bottomley, Steven P.; Whittington, Richard J.; Adler, Ben; Songer, J. Glenn; Rood, Julian I.; Paulsen, Ian T.

In: Nature Biotechnology, Vol. 25, No. 5, 01.05.2007, p. 569-575.

Research output: Contribution to journalArticle

Myers, GSA, Parker, D, Al-Hasani, K, Kennan, RM, Seemann, T, Ren, Q, Badger, JH, Selengut, JD, DeBoy, RT, Tettelin, H, Boyce, JD, McCarl, VP, Han, X, Nelson, WC, Madupu, R, Ali Mohamoud, Y, Holley, T, Fedorova, N, Khouri, H, Bottomley, SP, Whittington, RJ, Adler, B, Songer, JG, Rood, JI & Paulsen, IT 2007, 'Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus', Nature Biotechnology, vol. 25, no. 5, pp. 569-575. https://doi.org/10.1038/nbt1302
Myers, Garry S.A. ; Parker, Dane ; Al-Hasani, Keith ; Kennan, Ruth M. ; Seemann, Torsten ; Ren, Qinghu ; Badger, Jonathan H. ; Selengut, Jeremy D. ; DeBoy, Robert T. ; Tettelin, Hervé ; Boyce, John D. ; McCarl, Victoria P. ; Han, Xiaoyan ; Nelson, William C. ; Madupu, Ramana ; Ali Mohamoud, Yasmin ; Holley, Tara ; Fedorova, Nadia ; Khouri, Hoda ; Bottomley, Steven P. ; Whittington, Richard J. ; Adler, Ben ; Songer, J. Glenn ; Rood, Julian I. ; Paulsen, Ian T. / Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus. In: Nature Biotechnology. 2007 ; Vol. 25, No. 5. pp. 569-575.
@article{4bcfd013694b4ae79268d05ca4b04c2e,
title = "Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus",
abstract = "Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20{\%} of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.",
author = "Myers, {Garry S.A.} and Dane Parker and Keith Al-Hasani and Kennan, {Ruth M.} and Torsten Seemann and Qinghu Ren and Badger, {Jonathan H.} and Selengut, {Jeremy D.} and DeBoy, {Robert T.} and Herv{\'e} Tettelin and Boyce, {John D.} and McCarl, {Victoria P.} and Xiaoyan Han and Nelson, {William C.} and Ramana Madupu and {Ali Mohamoud}, Yasmin and Tara Holley and Nadia Fedorova and Hoda Khouri and Bottomley, {Steven P.} and Whittington, {Richard J.} and Ben Adler and Songer, {J. Glenn} and Rood, {Julian I.} and Paulsen, {Ian T.}",
year = "2007",
month = "5",
day = "1",
doi = "10.1038/nbt1302",
language = "English",
volume = "25",
pages = "569--575",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus

AU - Myers, Garry S.A.

AU - Parker, Dane

AU - Al-Hasani, Keith

AU - Kennan, Ruth M.

AU - Seemann, Torsten

AU - Ren, Qinghu

AU - Badger, Jonathan H.

AU - Selengut, Jeremy D.

AU - DeBoy, Robert T.

AU - Tettelin, Hervé

AU - Boyce, John D.

AU - McCarl, Victoria P.

AU - Han, Xiaoyan

AU - Nelson, William C.

AU - Madupu, Ramana

AU - Ali Mohamoud, Yasmin

AU - Holley, Tara

AU - Fedorova, Nadia

AU - Khouri, Hoda

AU - Bottomley, Steven P.

AU - Whittington, Richard J.

AU - Adler, Ben

AU - Songer, J. Glenn

AU - Rood, Julian I.

AU - Paulsen, Ian T.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

AB - Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the genome is derived from lateral gene transfer. Most of these regions seem to be associated with virulence. Metabolic reconstruction indicated unsuspected capabilities, including carbohydrate utilization, electron transfer and several aerobic pathways. Global transcriptional profiling and bioinformatic analysis enabled the prediction of virulence factors and cell surface proteins. Screening of these proteins against ovine antisera identified eight immunogenic proteins that are candidate antigens for a cross-protective vaccine.

UR - http://www.scopus.com/inward/record.url?scp=34249751624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249751624&partnerID=8YFLogxK

U2 - 10.1038/nbt1302

DO - 10.1038/nbt1302

M3 - Article

VL - 25

SP - 569

EP - 575

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 5

ER -