Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1

Mourad Sabri, Roman Häuser, Marc Ouellette, Jing Liu, Mohammed Dehbi, Greg Moeck, Ernesto García, Björn Titz, Peter Uetz, Sylvain Moineau

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Streptococcus pneumoniae causes several diseases, including pneumonia, septicemia, and meningitis. Phage Dp-1 is one of the very few isolated virulent S. pneumoniae bacteriophages, but only a partial characterization is currently available. Here, we confirmed that Dp-1 belongs to the family Siphoviridae. Then, we determined its complete genomic sequence of 56,506 bp. It encodes 72 open reading frames, of which 44 have been assigned a function. We have identified putative promoters, Rho-independent terminators, and several genomic clusters. We provide evidence that Dp-1 may be using a novel DNA replication system as well as redirecting host protein synthesis through queuosine-containing tRNAs. Liquid chromatography-mass spectrometry analysis of purified phage Dp-1 particles identified at least eight structural proteins. Finally, using comprehensive yeast two-hybrid screens, we identified 156 phage protein interactions, and this intraviral interactome was used to propose a structural model of Dp-1.

Original languageEnglish
Pages (from-to)551-562
Number of pages12
JournalJournal of Bacteriology
Volume193
Issue number2
DOIs
Publication statusPublished - 1 Jan 2011
Externally publishedYes

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Streptococcus pneumoniae
Bacteriophages
Genome
Nucleoside Q
Streptococcus Phages
Siphoviridae
Proteins
Structural Models
Transfer RNA
DNA Replication
Meningitis
Liquid Chromatography
Open Reading Frames
Mass Spectrometry
Sepsis
Pneumonia
Yeasts

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1. / Sabri, Mourad; Häuser, Roman; Ouellette, Marc; Liu, Jing; Dehbi, Mohammed; Moeck, Greg; García, Ernesto; Titz, Björn; Uetz, Peter; Moineau, Sylvain.

In: Journal of Bacteriology, Vol. 193, No. 2, 01.01.2011, p. 551-562.

Research output: Contribution to journalArticle

Sabri, M, Häuser, R, Ouellette, M, Liu, J, Dehbi, M, Moeck, G, García, E, Titz, B, Uetz, P & Moineau, S 2011, 'Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1', Journal of Bacteriology, vol. 193, no. 2, pp. 551-562. https://doi.org/10.1128/JB.01117-10
Sabri, Mourad ; Häuser, Roman ; Ouellette, Marc ; Liu, Jing ; Dehbi, Mohammed ; Moeck, Greg ; García, Ernesto ; Titz, Björn ; Uetz, Peter ; Moineau, Sylvain. / Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1. In: Journal of Bacteriology. 2011 ; Vol. 193, No. 2. pp. 551-562.
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