Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1

Mourad Sabri, Roman Häuser, Marc Ouellette, Jing Liu, Mohammed Dehbi, Greg Moeck, Ernesto García, Björn Titz, Peter Uetz, Sylvain Moineau

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Streptococcus pneumoniae causes several diseases, including pneumonia, septicemia, and meningitis. Phage Dp-1 is one of the very few isolated virulent S. pneumoniae bacteriophages, but only a partial characterization is currently available. Here, we confirmed that Dp-1 belongs to the family Siphoviridae. Then, we determined its complete genomic sequence of 56,506 bp. It encodes 72 open reading frames, of which 44 have been assigned a function. We have identified putative promoters, Rho-independent terminators, and several genomic clusters. We provide evidence that Dp-1 may be using a novel DNA replication system as well as redirecting host protein synthesis through queuosine-containing tRNAs. Liquid chromatography-mass spectrometry analysis of purified phage Dp-1 particles identified at least eight structural proteins. Finally, using comprehensive yeast two-hybrid screens, we identified 156 phage protein interactions, and this intraviral interactome was used to propose a structural model of Dp-1.

Original languageEnglish
Pages (from-to)551-562
Number of pages12
JournalJournal of Bacteriology
Volume193
Issue number2
DOIs
Publication statusPublished - 1 Jan 2011

    Fingerprint

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Sabri, M., Häuser, R., Ouellette, M., Liu, J., Dehbi, M., Moeck, G., García, E., Titz, B., Uetz, P., & Moineau, S. (2011). Genome annotation and intraviral interactome for the streptococcus pneumoniae virulent phage Dp-1. Journal of Bacteriology, 193(2), 551-562. https://doi.org/10.1128/JB.01117-10