Genetic variation of microsatellite markers D1S117, D6S89, D11S35, APOC2, and D21S168 in the Spanish population

Juan José Fuentes, Isabel Banchs, Victor Volpini, Xavier P. Estivill

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11 Citations (Scopus)

Abstract

We have used PCR amplification to analyse the allele frequency, distribution and heterozygosity of 5 microsatellite markers (D1S117, D6S89, D11S35, APOC2, and D21S168), in a sample of 100 unrelated Spanish individuals. The loci tested exhibit wide allelic variability having 7-17 alleles, PIC (polymorphic information content) between 0.79 and 0.86, and heterozygosity between 0.81 and 0.86. D1S117 and D21S168 have unimodal distribution, APOC2 has 4 common alleles which account for 71% of the total variation, D11S35 has a bimodal distribution and D6S89 is trimodal. The allelic distribution observed for each locus is in agreement with slippage and mispairing as the main mechanisms involved in the evolution of microsatellite alleles. Multiplex amplification of loci D6S89 and APOC2 was possible due to their non-overlapping allele sizes. The rapidity with which microsatellites can be analysed, and the accurate determination of alleles, make these markers very powerful tools for genetic typing. The information obtained for loci D1S117, D6S89, D11S35, APOC2, and D21S168, provides a basis for their use for DNA typing and paternity analysis in the Spanish population.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalInternational Journal of Legal Medicine
Volume105
Issue number5
DOIs
Publication statusPublished - Sep 1993
Externally publishedYes

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Keywords

  • Microsatellites
  • Paternity testing
  • Polymorphisms
  • VNTR

ASJC Scopus subject areas

  • Law
  • Pathology and Forensic Medicine
  • Medicine (miscellaneous)

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