Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity

Lotfi Chouchane, J. Danguir, C. Beji, K. Bouassida, L. Camoin, H. Sfar, S. Gabbouj, A. D. Strosberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

BACKGROUND: Tumor necrosis factor-α (TNF-α) expression is increased in adipose tissue of both rodent models of obesity and obese humans. It has therefore been considered as a candidate gene for obesity. Several studies have indeed shown statistical evidence of linkage between obesity and the chromosomal region encompassing the TNF-α gene, suggesting that TNF-α and/or a nearby gene (eg hsp70 gene) is involved in the onset and progression of weight gain. We designed a case-controlled study to investigate the potential association of polymorphism of the TNF-α and that of a stress protein (hsp70-2) with obesity. METHODS: We used the polymerase chain reaction and restriction enzyme digestion to characterize the variation of the TNF-α promoter region and that of the hsp70-2 gene in 343 unrelated Tunisian patients with obesity and 174 healthy control subiects. RESULTS: Analysis of the -308 TNF-α polymorphism in patients with obesity and in control subjects did not reveal an association between TNF-α alleles and obesity. In contrast, polymorphism analysis of the hsp70-2 gene in patients with obesity demonstrated highly significant differences in genotypic distribution of this bi-allelic locus compared to the control subject group. Homozygosity for one hsp70-2 allele was highly associated with obesity (r2 = 7.12; P < 10-6). CONCLUSION: Tunisian persons carrying the P2/P2 genotype of the hsp70-2 gene may have an increased risk of obesity.

Original languageEnglish
Pages (from-to)462-466
Number of pages5
JournalInternational Journal of Obesity
Volume25
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Heat-Shock Proteins
Obesity
Tumor Necrosis Factor-alpha
Genes
Alleles
Genetic Promoter Regions
Weight Gain
Adipose Tissue
Digestion
Rodentia
Genotype
Polymerase Chain Reaction
Control Groups
Enzymes

Keywords

  • Obesity
  • Polymorphism
  • Stress proteins
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity. / Chouchane, Lotfi; Danguir, J.; Beji, C.; Bouassida, K.; Camoin, L.; Sfar, H.; Gabbouj, S.; Strosberg, A. D.

In: International Journal of Obesity, Vol. 25, No. 4, 2001, p. 462-466.

Research output: Contribution to journalArticle

Chouchane, L, Danguir, J, Beji, C, Bouassida, K, Camoin, L, Sfar, H, Gabbouj, S & Strosberg, AD 2001, 'Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity', International Journal of Obesity, vol. 25, no. 4, pp. 462-466. https://doi.org/10.1038/sj.ijo.0801545
Chouchane, Lotfi ; Danguir, J. ; Beji, C. ; Bouassida, K. ; Camoin, L. ; Sfar, H. ; Gabbouj, S. ; Strosberg, A. D. / Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity. In: International Journal of Obesity. 2001 ; Vol. 25, No. 4. pp. 462-466.
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