Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity

Lotfi Chouchane, J. Danguir, C. Beji, K. Bouassida, L. Camoin, H. Sfar, S. Gabbouj, A. D. Strosberg

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BACKGROUND: Tumor necrosis factor-α (TNF-α) expression is increased in adipose tissue of both rodent models of obesity and obese humans. It has therefore been considered as a candidate gene for obesity. Several studies have indeed shown statistical evidence of linkage between obesity and the chromosomal region encompassing the TNF-α gene, suggesting that TNF-α and/or a nearby gene (eg hsp70 gene) is involved in the onset and progression of weight gain. We designed a case-controlled study to investigate the potential association of polymorphism of the TNF-α and that of a stress protein (hsp70-2) with obesity. METHODS: We used the polymerase chain reaction and restriction enzyme digestion to characterize the variation of the TNF-α promoter region and that of the hsp70-2 gene in 343 unrelated Tunisian patients with obesity and 174 healthy control subiects. RESULTS: Analysis of the -308 TNF-α polymorphism in patients with obesity and in control subjects did not reveal an association between TNF-α alleles and obesity. In contrast, polymorphism analysis of the hsp70-2 gene in patients with obesity demonstrated highly significant differences in genotypic distribution of this bi-allelic locus compared to the control subject group. Homozygosity for one hsp70-2 allele was highly associated with obesity (r2 = 7.12; P < 10-6). CONCLUSION: Tunisian persons carrying the P2/P2 genotype of the hsp70-2 gene may have an increased risk of obesity.

Original languageEnglish
Pages (from-to)462-466
Number of pages5
JournalInternational Journal of Obesity
Issue number4
Publication statusPublished - 2001
Externally publishedYes



  • Obesity
  • Polymorphism
  • Stress proteins
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Chouchane, L., Danguir, J., Beji, C., Bouassida, K., Camoin, L., Sfar, H., Gabbouj, S., & Strosberg, A. D. (2001). Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity. International Journal of Obesity, 25(4), 462-466.