Genetic Variation in IL-8 Associated with Increased Risk and Poor Prognosis of Breast Carcinoma

Kaouther Snoussi, Wijden Mahfoudh, Noureddine Bouaouina, Slim Ben Ahmed, A. Noureddine Helal, Lotfi Chouchane

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Interleukin-8 (IL-8), a potent chemoattractant, has been demonstrated to contribute to human cancer progression through its potential functions as a mitogenic, angiogenic, and motogenic factor. We designed a broad study to investigate whether genetic variation in IL-8 has implications for susceptibility to and prognosis in breast carcinoma. We used the allele-specific polymerase chain reaction to characterize the variation of the IL-8 promoter region for 308 unrelated Tunisian patients with breast carcinoma and 236 healthy control subjects. Associations of the clinicopathologic parameters and the genetic marker with the rates of the breast carcinoma-specific overall survival and the disease-free survival were assessed using univariate and multivariate analyses. A significantly increased risk of breast carcinoma was associated with heterozygous IL-8 (-251) TA (OR = 1.58, p = 0.02) and homozygous IL-8 (-251) AA (OR = 1.76, p = 0.01) variants. A significant association between the IL-8 (-251) AA homozygous genotype and the aggressive phenotype of breast carcinoma as defined by the high histological grade, auxiliary's lymph node metastasis, and large tumor size was found. The IL-8 (-251) A allele manifested a significant association with decreased overall survival and disease-free survival for breast carcinoma patients. The polymorphism in the promoter region of the IL-8 gene may not only represent a marker for the increased risk of breast carcinoma but also predict the clinical outcome.

Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalHuman Immunology
Volume67
Issue number1-2
DOIs
Publication statusPublished - Jan 2006
Externally publishedYes

Fingerprint

Interleukin-8
Breast Neoplasms
Genetic Promoter Regions
Disease-Free Survival
Alleles
Survival
Angiogenesis Inducing Agents
Chemotactic Factors
Genetic Markers
Neoplasms
Healthy Volunteers
Multivariate Analysis
Lymph Nodes
Genotype
Neoplasm Metastasis
Phenotype
Polymerase Chain Reaction
Genes

Keywords

  • Breast carcinoma
  • interleukin-8
  • polymorphism
  • prognosis
  • susceptibility

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Genetic Variation in IL-8 Associated with Increased Risk and Poor Prognosis of Breast Carcinoma. / Snoussi, Kaouther; Mahfoudh, Wijden; Bouaouina, Noureddine; Ahmed, Slim Ben; Helal, A. Noureddine; Chouchane, Lotfi.

In: Human Immunology, Vol. 67, No. 1-2, 01.2006, p. 13-21.

Research output: Contribution to journalArticle

Snoussi, Kaouther ; Mahfoudh, Wijden ; Bouaouina, Noureddine ; Ahmed, Slim Ben ; Helal, A. Noureddine ; Chouchane, Lotfi. / Genetic Variation in IL-8 Associated with Increased Risk and Poor Prognosis of Breast Carcinoma. In: Human Immunology. 2006 ; Vol. 67, No. 1-2. pp. 13-21.
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