Genetic relatedness among Shiga toxin-producing Escherichia coli isolated along the animal food supply chain and in gastroenteritis cases in Qatar using multilocus sequence typing

Srikanth Palanisamy, Yu Chen Chang, Joy Scaria, Rafael Antonio Casarin Penha Filho, Kenlyn E. Peters, Sanjay H. Doiphode, Ali Sultan, Hussni O. Mohammed

Research output: Contribution to journalArticle

Abstract

Introduction: Pathogenic Escherichia coli has been listed among the most important bacteria associated with foodborne illnesses around the world. We investigated the genetic relatedness among Shiga toxin-producing E. coli (STEC) isolated along the animal food supply chain and from humans diagnosed with gastroenteritis in Qatar. Methods: Samples were collected from different sources along the food supply chain and from patients admitted to the hospital with complaints of gastroenteritis. All samples were screened for the presence of E. coli O157:H7 and non-O157 STEC using a combination of bacterial enrichment and molecular detection techniques. A proportional sampling approach was used to select positive samples from each source for further multilocus sequence typing (MLST) analysis. Seven housekeeping genes described for STEC were amplified by polymerase chain reaction, sequenced, and analyzed by MLST. Isolates were characterized by allele composition, sequence type (ST) and assessed for epidemiologic relationship within and among different sources. Nei's genetic distance was calculated at the allele level between sample pools in each site downstream. Results: E. coli O157:H7 occurred at a higher rate in slaughterhouse and retail samples than at the farm or in humans in our sampling. The ST171, an ST common to enterotoxigenic E. coli and atypical enteropathogenic E. coli, was the most common ST (15%) in the food supply chain. None of the genetic distances among the different sources was statistically significant. Conclusion: Enterohemorrhagic E. coli pathogenic strains are present along the supply chain at different levels and with varying relatedness. Clinical isolates were the most diverse, as expected, considering the polyclonal diversity in the human microbiota. The high occurrence of these food adulterants among the farm products suggests that implementation of sanitary measures at that level might reduce the risk of human exposure.

Original languageEnglish
Pages (from-to)318-325
Number of pages8
JournalFoodborne Pathogens and Disease
Volume14
Issue number6
DOIs
Publication statusPublished - 1 Jun 2017

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Qatar
Shiga-Toxigenic Escherichia coli
Multilocus Sequence Typing
Shiga toxin-producing Escherichia coli
Food Chain
Food Supply
gastroenteritis
Gastroenteritis
food supply chain
genetic relationships
Escherichia coli O157
animals
Alleles
Enterohemorrhagic Escherichia coli
Enterotoxigenic Escherichia coli
Enteropathogenic Escherichia coli
sampling
Abattoirs
Foodborne Diseases
Microbiota

Keywords

  • E. coliO157:H7
  • epidemiology
  • food adulterants
  • food chain
  • gastroenteritis (foodborne illness),MLST

ASJC Scopus subject areas

  • Microbiology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

Cite this

Genetic relatedness among Shiga toxin-producing Escherichia coli isolated along the animal food supply chain and in gastroenteritis cases in Qatar using multilocus sequence typing. / Palanisamy, Srikanth; Chang, Yu Chen; Scaria, Joy; Penha Filho, Rafael Antonio Casarin; Peters, Kenlyn E.; Doiphode, Sanjay H.; Sultan, Ali; Mohammed, Hussni O.

In: Foodborne Pathogens and Disease, Vol. 14, No. 6, 01.06.2017, p. 318-325.

Research output: Contribution to journalArticle

Palanisamy, Srikanth ; Chang, Yu Chen ; Scaria, Joy ; Penha Filho, Rafael Antonio Casarin ; Peters, Kenlyn E. ; Doiphode, Sanjay H. ; Sultan, Ali ; Mohammed, Hussni O. / Genetic relatedness among Shiga toxin-producing Escherichia coli isolated along the animal food supply chain and in gastroenteritis cases in Qatar using multilocus sequence typing. In: Foodborne Pathogens and Disease. 2017 ; Vol. 14, No. 6. pp. 318-325.
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abstract = "Introduction: Pathogenic Escherichia coli has been listed among the most important bacteria associated with foodborne illnesses around the world. We investigated the genetic relatedness among Shiga toxin-producing E. coli (STEC) isolated along the animal food supply chain and from humans diagnosed with gastroenteritis in Qatar. Methods: Samples were collected from different sources along the food supply chain and from patients admitted to the hospital with complaints of gastroenteritis. All samples were screened for the presence of E. coli O157:H7 and non-O157 STEC using a combination of bacterial enrichment and molecular detection techniques. A proportional sampling approach was used to select positive samples from each source for further multilocus sequence typing (MLST) analysis. Seven housekeeping genes described for STEC were amplified by polymerase chain reaction, sequenced, and analyzed by MLST. Isolates were characterized by allele composition, sequence type (ST) and assessed for epidemiologic relationship within and among different sources. Nei's genetic distance was calculated at the allele level between sample pools in each site downstream. Results: E. coli O157:H7 occurred at a higher rate in slaughterhouse and retail samples than at the farm or in humans in our sampling. The ST171, an ST common to enterotoxigenic E. coli and atypical enteropathogenic E. coli, was the most common ST (15{\%}) in the food supply chain. None of the genetic distances among the different sources was statistically significant. Conclusion: Enterohemorrhagic E. coli pathogenic strains are present along the supply chain at different levels and with varying relatedness. Clinical isolates were the most diverse, as expected, considering the polyclonal diversity in the human microbiota. The high occurrence of these food adulterants among the farm products suggests that implementation of sanitary measures at that level might reduce the risk of human exposure.",
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AU - Mohammed, Hussni O.

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