Generation and characterization of monoclonal antibodies to protective antigen of Bacillus anthracis

Seetharama S. Konduru, U. Tuteja, H. V. Batra

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Monoclonal antibodies (MoAbs) were generated following immunization of BALB/c mice with protective antigen (PA) of B. anthracis. Five clones reactive to this protein were stabilized and preserved. These MoAbs could detect nanogram levels of PA when tested in ELISA. In Western blotting, they reacted with all PA preparations tested and no cross-reactivity was observed with lethal factor, edema factor of B. anthracis and with other organisms. These MoAbs could detect PA from 22 confirmed clinical isolates of B. anthracis on Western blotting and hold promise for direct detection of PA in clinical samples for diagnosing anthrax.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalIndian Journal of Experimental Biology
Volume41
Issue number2
Publication statusPublished - 1 Feb 2003
Externally publishedYes

Fingerprint

Monoclonal antibodies
Bacilli
Antigens
Monoclonal Antibodies
Western Blotting
Bacillus anthracis
Anthrax
Immunization
Clone Cells
Enzyme-Linked Immunosorbent Assay
anthrax toxin
Proteins

ASJC Scopus subject areas

  • Bioengineering

Cite this

Generation and characterization of monoclonal antibodies to protective antigen of Bacillus anthracis. / Konduru, Seetharama S.; Tuteja, U.; Batra, H. V.

In: Indian Journal of Experimental Biology, Vol. 41, No. 2, 01.02.2003, p. 123-128.

Research output: Contribution to journalArticle

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