Gene expression signatures in breast cancer distinguish phenotype characteristics, histologic subtypes, and tumor invasiveness

Vincente Pedraza, Jose A. Gomez-Capilla, Georgia Escaramis, Carolina Gomez, Pablo Torné, Jose M. Rivera, Angel Gil, Patricia Araque, Nicolas Olea, Xavier P. Estivill, M. Esther Fárez-Vidal

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34 Citations (Scopus)

Abstract

BACKGROUND: The development of reliable gene expression profiling technology is having an increasing impact on the understanding of breast cancer biology. METHODS: In this study, microarray analysis was performed to establish gene signatures for different breast cancer phenotypes, to determine differentially expressed gene sequences at different stages of the disease, and to identify sequences with biologic significance for tumor progression. Samples were taken from patients before their treatment. After microarray analysis, the expression level of 153 selected genes was studied by real-time quantitative polymerase chain reaction analysis. RESULTS: Several gene sequences were expressed differentially in tumor samples versus control samples and also were associated with different breast cancer phenotypes, estrogen receptor status, tumor histology, and grade of tumor differentiation. In lymph node-negative tumors were identified a set of genes related to tumor differentiation grade. CONCLUSIONS: Several differentially expressed gene sequences were identified at different stages of breast cancer.

Original languageEnglish
Pages (from-to)486-496
Number of pages11
JournalCancer
Volume116
Issue number2
DOIs
Publication statusPublished - 15 Jan 2010
Externally publishedYes

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Keywords

  • Breast cancer
  • Gene expression signature
  • Microarrays
  • Real-time quantitative polymerase chain reaction
  • Tumor invasiveness

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Pedraza, V., Gomez-Capilla, J. A., Escaramis, G., Gomez, C., Torné, P., Rivera, J. M., Gil, A., Araque, P., Olea, N., Estivill, X. P., & Fárez-Vidal, M. E. (2010). Gene expression signatures in breast cancer distinguish phenotype characteristics, histologic subtypes, and tumor invasiveness. Cancer, 116(2), 486-496. https://doi.org/10.1002/cncr.24805