Gene-expression profiling of the response of peripheral blood mononuclear cells and melanoma metastases to systemic IL-2 administration.

Monica C. Panelli, Ena Wang, Giao Phan, Markus Puhlmann, Lance Miller, Galen A. Ohnmacht, Harvey G. Klein, Francesco M. Marincola

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Interleukin-2 (IL-2) has direct pluripotent effects on cells with immune and inflammatory function. Which of these effects has a critical role in mediating tumor regression remains enigmatic. In this study, we compared early changes in transcriptional profiles of circulating mononuclear cells with those occurring within the microenvironment of melanoma metastases following systemic IL-2 administration. RESULTS: The results suggest that the immediate effects of IL-2 administration on the tumor microenvironment is transcriptional activation of genes predominantly associated with monocyte cell function; minimal effects were noted on migration, activation and proliferation of T cells. However, production of chemokines and markers of adhesion and migration within few hours of IL-2 administration may be responsible for a secondary recruitment of immune cells to the tumor site later. CONCLUSIONS:Our results suggest that IL-2 induces inflammation at tumor sites with three predominant secondary effects: activation of antigen-presenting monocytes; massive production of chemoattractants that may recruit other immune cells to the tumor (including MIG and PARC, which are specific for T cells); and activation of cytolytic mechanisms in monocytes (calgranulin, grancalcin) and NK cells (NKG5, NK4).

Original languageEnglish
JournalGenome Biology
Volume3
Issue number7
Publication statusPublished - 25 Jun 2002
Externally publishedYes

Fingerprint

Gene Expression Profiling
melanoma
mononuclear leukocytes
interleukin-2
tumor
metastasis
gene expression
Interleukin-2
Melanoma
Blood Cells
blood
Neoplasm Metastasis
monocytes
Monocytes
neoplasms
cells
Neoplasms
T-lymphocytes
Leukocyte L1 Antigen Complex
T-Lymphocytes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

Cite this

Panelli, M. C., Wang, E., Phan, G., Puhlmann, M., Miller, L., Ohnmacht, G. A., ... Marincola, F. M. (2002). Gene-expression profiling of the response of peripheral blood mononuclear cells and melanoma metastases to systemic IL-2 administration. Genome Biology, 3(7).

Gene-expression profiling of the response of peripheral blood mononuclear cells and melanoma metastases to systemic IL-2 administration. / Panelli, Monica C.; Wang, Ena; Phan, Giao; Puhlmann, Markus; Miller, Lance; Ohnmacht, Galen A.; Klein, Harvey G.; Marincola, Francesco M.

In: Genome Biology, Vol. 3, No. 7, 25.06.2002.

Research output: Contribution to journalArticle

Panelli, MC, Wang, E, Phan, G, Puhlmann, M, Miller, L, Ohnmacht, GA, Klein, HG & Marincola, FM 2002, 'Gene-expression profiling of the response of peripheral blood mononuclear cells and melanoma metastases to systemic IL-2 administration.', Genome Biology, vol. 3, no. 7.
Panelli, Monica C. ; Wang, Ena ; Phan, Giao ; Puhlmann, Markus ; Miller, Lance ; Ohnmacht, Galen A. ; Klein, Harvey G. ; Marincola, Francesco M. / Gene-expression profiling of the response of peripheral blood mononuclear cells and melanoma metastases to systemic IL-2 administration. In: Genome Biology. 2002 ; Vol. 3, No. 7.
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