Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning

Henrik Fagman, Elena Amendola, Luca Parrillo, Pietro Zoppoli, Pina Marotta, Marzia Scarfò, Pasquale de Luca, Denise Pires de Carvalho, Michele Ceccarelli, Mario de Felice, Roberto di Lauro

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.

Original languageEnglish
Pages (from-to)163-175
Number of pages13
JournalDevelopmental Biology
Volume359
Issue number2
DOIs
Publication statusPublished - 15 Nov 2011
Externally publishedYes

Fingerprint

Organogenesis
Gene Expression Profiling
Thyroid Gland
Lung
Genes
Laser Capture Microdissection
Microarray Analysis
Morphogenesis
Transcriptome
Cell Survival
Embryonic Structures
Gene Expression

Keywords

  • Foregut patterning
  • LCM
  • Lung bud
  • Microarrays
  • Organogenesis
  • Thyroid bud

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Fagman, H., Amendola, E., Parrillo, L., Zoppoli, P., Marotta, P., Scarfò, M., ... di Lauro, R. (2011). Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning. Developmental Biology, 359(2), 163-175. https://doi.org/10.1016/j.ydbio.2011.08.015

Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning. / Fagman, Henrik; Amendola, Elena; Parrillo, Luca; Zoppoli, Pietro; Marotta, Pina; Scarfò, Marzia; de Luca, Pasquale; de Carvalho, Denise Pires; Ceccarelli, Michele; de Felice, Mario; di Lauro, Roberto.

In: Developmental Biology, Vol. 359, No. 2, 15.11.2011, p. 163-175.

Research output: Contribution to journalArticle

Fagman, H, Amendola, E, Parrillo, L, Zoppoli, P, Marotta, P, Scarfò, M, de Luca, P, de Carvalho, DP, Ceccarelli, M, de Felice, M & di Lauro, R 2011, 'Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning', Developmental Biology, vol. 359, no. 2, pp. 163-175. https://doi.org/10.1016/j.ydbio.2011.08.015
Fagman, Henrik ; Amendola, Elena ; Parrillo, Luca ; Zoppoli, Pietro ; Marotta, Pina ; Scarfò, Marzia ; de Luca, Pasquale ; de Carvalho, Denise Pires ; Ceccarelli, Michele ; de Felice, Mario ; di Lauro, Roberto. / Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning. In: Developmental Biology. 2011 ; Vol. 359, No. 2. pp. 163-175.
@article{d7da95f297fc48ce80dbf947a53bb01e,
title = "Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning",
abstract = "The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.",
keywords = "Foregut patterning, LCM, Lung bud, Microarrays, Organogenesis, Thyroid bud",
author = "Henrik Fagman and Elena Amendola and Luca Parrillo and Pietro Zoppoli and Pina Marotta and Marzia Scarf{\`o} and {de Luca}, Pasquale and {de Carvalho}, {Denise Pires} and Michele Ceccarelli and {de Felice}, Mario and {di Lauro}, Roberto",
year = "2011",
month = "11",
day = "15",
doi = "10.1016/j.ydbio.2011.08.015",
language = "English",
volume = "359",
pages = "163--175",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning

AU - Fagman, Henrik

AU - Amendola, Elena

AU - Parrillo, Luca

AU - Zoppoli, Pietro

AU - Marotta, Pina

AU - Scarfò, Marzia

AU - de Luca, Pasquale

AU - de Carvalho, Denise Pires

AU - Ceccarelli, Michele

AU - de Felice, Mario

AU - di Lauro, Roberto

PY - 2011/11/15

Y1 - 2011/11/15

N2 - The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.

AB - The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.

KW - Foregut patterning

KW - LCM

KW - Lung bud

KW - Microarrays

KW - Organogenesis

KW - Thyroid bud

UR - http://www.scopus.com/inward/record.url?scp=82455175320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82455175320&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2011.08.015

DO - 10.1016/j.ydbio.2011.08.015

M3 - Article

C2 - 21924257

AN - SCOPUS:82455175320

VL - 359

SP - 163

EP - 175

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -