Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients

Lars F. Westblade, Michael J. Satlin, Shady Albakry, Brittany Botticelli, Amy Robertson, Tricia Alston, Matthew Magruder, Lisa T. Zhang, Emmanuel Edusei, Kevin Chan, Michelle Lubetzky, Darshana M. Dadhania, Eric G. Pamer, Manikkam Suthanthiran, John R. Lee

Research output: Contribution to journalArticle

Abstract

Background: In kidney transplant recipients, gastrointestinal (GI) pathogens in feces are only evaluated during diarrheal episodes. Little is known about the prevalence of GI pathogens in asymptomatic individuals in this population. Methods: We recruited 142 kidney transplant recipients who provided a non-diarrheal fecal sample within the first 10 days after transplantation. The specimens were evaluated for GI pathogens using the BioFire® FilmArray® GI Panel (BioFire Diagnostics, LLC), which tests for 22 pathogens. The fecal microbiome was also characterized using 16S rRNA gene sequencing of the V4-V5 hypervariable region. We evaluated whether detection of Clostridioides difficile and other GI pathogens was associated with post-transplant diarrhea within the first 3 months after transplantation. Results: Among the 142 subjects, a potential pathogen was detected in 43 (30%) using the GI Panel. The most common organisms detected were C difficile (n = 24, 17%), enteropathogenic Escherichia coli (n = 8, 6%), and norovirus (n = 5, 4%). Detection of a pathogen on the GI panel or detection of C difficile alone was not associated with future post-transplant diarrhea (P >.05). The estimated number of gut bacterial species was significantly lower in subjects colonized with C difficile than those not colonized with a GI pathogen (P =.01). Conclusion: Colonization with GI pathogens, particularly C difficile, is common at the time of kidney transplantation but does not predict subsequent diarrhea. Detection of C difficile carriage was associated with decreased microbial diversity and may be a biomarker of gut dysbiosis.

Original languageEnglish
Article numbere13167
JournalTransplant Infectious Disease
DOIs
Publication statusAccepted/In press - 1 Jan 2019

Fingerprint

Microbiota
Diarrhea
Kidney
Transplantation
Dysbiosis
Transplants
Norovirus
Enteropathogenic Escherichia coli
rRNA Genes
Feces
Kidney Transplantation
Biomarkers
Population
Transplant Recipients

Keywords

  • Clostridioides difficile
  • colonization
  • diarrhea
  • gastrointestinal panel
  • kidney transplantation
  • microbiome

ASJC Scopus subject areas

  • Infectious Diseases
  • Transplantation

Cite this

Westblade, L. F., Satlin, M. J., Albakry, S., Botticelli, B., Robertson, A., Alston, T., ... Lee, J. R. (Accepted/In press). Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients. Transplant Infectious Disease, [e13167]. https://doi.org/10.1111/tid.13167

Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients. / Westblade, Lars F.; Satlin, Michael J.; Albakry, Shady; Botticelli, Brittany; Robertson, Amy; Alston, Tricia; Magruder, Matthew; Zhang, Lisa T.; Edusei, Emmanuel; Chan, Kevin; Lubetzky, Michelle; Dadhania, Darshana M.; Pamer, Eric G.; Suthanthiran, Manikkam; Lee, John R.

In: Transplant Infectious Disease, 01.01.2019.

Research output: Contribution to journalArticle

Westblade, LF, Satlin, MJ, Albakry, S, Botticelli, B, Robertson, A, Alston, T, Magruder, M, Zhang, LT, Edusei, E, Chan, K, Lubetzky, M, Dadhania, DM, Pamer, EG, Suthanthiran, M & Lee, JR 2019, 'Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients', Transplant Infectious Disease. https://doi.org/10.1111/tid.13167
Westblade, Lars F. ; Satlin, Michael J. ; Albakry, Shady ; Botticelli, Brittany ; Robertson, Amy ; Alston, Tricia ; Magruder, Matthew ; Zhang, Lisa T. ; Edusei, Emmanuel ; Chan, Kevin ; Lubetzky, Michelle ; Dadhania, Darshana M. ; Pamer, Eric G. ; Suthanthiran, Manikkam ; Lee, John R. / Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients. In: Transplant Infectious Disease. 2019.
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abstract = "Background: In kidney transplant recipients, gastrointestinal (GI) pathogens in feces are only evaluated during diarrheal episodes. Little is known about the prevalence of GI pathogens in asymptomatic individuals in this population. Methods: We recruited 142 kidney transplant recipients who provided a non-diarrheal fecal sample within the first 10 days after transplantation. The specimens were evaluated for GI pathogens using the BioFire{\circledR} FilmArray{\circledR} GI Panel (BioFire Diagnostics, LLC), which tests for 22 pathogens. The fecal microbiome was also characterized using 16S rRNA gene sequencing of the V4-V5 hypervariable region. We evaluated whether detection of Clostridioides difficile and other GI pathogens was associated with post-transplant diarrhea within the first 3 months after transplantation. Results: Among the 142 subjects, a potential pathogen was detected in 43 (30{\%}) using the GI Panel. The most common organisms detected were C difficile (n = 24, 17{\%}), enteropathogenic Escherichia coli (n = 8, 6{\%}), and norovirus (n = 5, 4{\%}). Detection of a pathogen on the GI panel or detection of C difficile alone was not associated with future post-transplant diarrhea (P >.05). The estimated number of gut bacterial species was significantly lower in subjects colonized with C difficile than those not colonized with a GI pathogen (P =.01). Conclusion: Colonization with GI pathogens, particularly C difficile, is common at the time of kidney transplantation but does not predict subsequent diarrhea. Detection of C difficile carriage was associated with decreased microbial diversity and may be a biomarker of gut dysbiosis.",
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AU - Satlin, Michael J.

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AU - Robertson, Amy

AU - Alston, Tricia

AU - Magruder, Matthew

AU - Zhang, Lisa T.

AU - Edusei, Emmanuel

AU - Chan, Kevin

AU - Lubetzky, Michelle

AU - Dadhania, Darshana M.

AU - Pamer, Eric G.

AU - Suthanthiran, Manikkam

AU - Lee, John R.

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N2 - Background: In kidney transplant recipients, gastrointestinal (GI) pathogens in feces are only evaluated during diarrheal episodes. Little is known about the prevalence of GI pathogens in asymptomatic individuals in this population. Methods: We recruited 142 kidney transplant recipients who provided a non-diarrheal fecal sample within the first 10 days after transplantation. The specimens were evaluated for GI pathogens using the BioFire® FilmArray® GI Panel (BioFire Diagnostics, LLC), which tests for 22 pathogens. The fecal microbiome was also characterized using 16S rRNA gene sequencing of the V4-V5 hypervariable region. We evaluated whether detection of Clostridioides difficile and other GI pathogens was associated with post-transplant diarrhea within the first 3 months after transplantation. Results: Among the 142 subjects, a potential pathogen was detected in 43 (30%) using the GI Panel. The most common organisms detected were C difficile (n = 24, 17%), enteropathogenic Escherichia coli (n = 8, 6%), and norovirus (n = 5, 4%). Detection of a pathogen on the GI panel or detection of C difficile alone was not associated with future post-transplant diarrhea (P >.05). The estimated number of gut bacterial species was significantly lower in subjects colonized with C difficile than those not colonized with a GI pathogen (P =.01). Conclusion: Colonization with GI pathogens, particularly C difficile, is common at the time of kidney transplantation but does not predict subsequent diarrhea. Detection of C difficile carriage was associated with decreased microbial diversity and may be a biomarker of gut dysbiosis.

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