G-quadruplex RNA structure as a signal for neurite mRNA targeting

Murugan Subramanian, Florence Rage, Ricardos Tabet, Eric Flatter, Jean Louis Mandel, Hervé Moine

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis-acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best-known dendritic mRNAs contain a guanine (G)quadruplex consensus in their 3-2-untranslated region. Among these mRNAs, we show by using RNA structure probing that a Gquadruplex is present in the mRNAs of two key postsynaptic proteins: PSD-95 and CaMKIIa. The Gquadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor-responsive manner. Thus, Gquadruplex seems to be a common neurite localization signal.

Original languageEnglish
Pages (from-to)697-704
Number of pages8
JournalEMBO Reports
Volume12
Issue number7
DOIs
Publication statusPublished - 1 Jul 2011
Externally publishedYes

Fingerprint

G-Quadruplexes
Neurites
RNA
Messenger RNA
Untranslated Regions
Metabotropic Glutamate Receptors
Guanine
3' Untranslated Regions
Neurons
Proteins

Keywords

  • CaMKIIa
  • FMRP
  • Guanine-quadruplex
  • mRNA trafficking
  • PSD-95

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Subramanian, M., Rage, F., Tabet, R., Flatter, E., Mandel, J. L., & Moine, H. (2011). G-quadruplex RNA structure as a signal for neurite mRNA targeting. EMBO Reports, 12(7), 697-704. https://doi.org/10.1038/embor.2011.76

G-quadruplex RNA structure as a signal for neurite mRNA targeting. / Subramanian, Murugan; Rage, Florence; Tabet, Ricardos; Flatter, Eric; Mandel, Jean Louis; Moine, Hervé.

In: EMBO Reports, Vol. 12, No. 7, 01.07.2011, p. 697-704.

Research output: Contribution to journalArticle

Subramanian, M, Rage, F, Tabet, R, Flatter, E, Mandel, JL & Moine, H 2011, 'G-quadruplex RNA structure as a signal for neurite mRNA targeting', EMBO Reports, vol. 12, no. 7, pp. 697-704. https://doi.org/10.1038/embor.2011.76
Subramanian, Murugan ; Rage, Florence ; Tabet, Ricardos ; Flatter, Eric ; Mandel, Jean Louis ; Moine, Hervé. / G-quadruplex RNA structure as a signal for neurite mRNA targeting. In: EMBO Reports. 2011 ; Vol. 12, No. 7. pp. 697-704.
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