Functional significance of anti-T-lymphocyte antibodies in sarcoidosis

J. R. Spurzem, C. Saltini, Ronald Crystal

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pulmonary sarcoidosis is a chronic disorder characterized by the activation of helper/inducer T-cells in the lung without a concomitant increase in suppressor/cytotoxic T-cells. It is known that patients with sarcoidosis have circulating anti-T-cell antibodies, primarily of the IgM class. To evaluate a functional role for these antibodies in enhancing lung helper T-cell processes in pulmonary sarcoidosis, we evaluated serum and lavage fluid of patients with active sarcoidosis for the presence of anti-T-cell antibodies, the T-cell subset specificity of these antibodies, and the possible stimulatory or inhibitory effects of these antibodies on T-cells relevant to the exaggerated helper T-cell processes in sarcoidosis. Indirect immunofluorescence studies demonstrated that sarcoid patients had anti-T-cell antibodies of the IgM type reacting with autologous as well as with nonautologous normal T-cells. IgM recovered in sarcoid lavage fluid also reacted with T-cells, thus demonstrating the autoantibodies at the site of disease. Two-color immunofluorescence and flow cytometry showed that these sarcoid autoantibodies bound to mostly Leu2+ suppressor/cytotoxic T-cells, but also to a small proportion of Leu3+ helper/inducer T-cells. Incubating lymphocytes with sarcoid serum or IgM purified from sarcoid serum did not stimulate T-cell proliferation. Furthermore, when Leu2+ T-cells were stimulated with irradiated allogenic B-cells, increasing concentrations of sarcoid serum had no inhibitory effects on the activation and proliferative response of the Leu2+ T-cells. Likewise, the purified IgM anti-T-cell antibodies had no inhibitory effects on the mitogenic response of Leu2+ T-cells to the anti-T-cell antigen receptor-associated T3 complex antibody OKT3. Thus, the IgM anti-T-cell autoantibodies of sarcoidosis are present at the site of disease and bind to potentially relevant T-cell subsets; however, our study found no evidence that they have an identifiable functional role in the development of the excess helper T cell activity of sarcoidosis.

Original languageEnglish
Pages (from-to)600-605
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume137
Issue number3
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

Fingerprint

Sarcoidosis
T-Lymphocytes
Antibodies
Immunoglobulin M
Helper-Inducer T-Lymphocytes
Autoantibodies
Pulmonary Sarcoidosis
Therapeutic Irrigation
T-Lymphocyte Subsets
Thomsen-Friedenreich antibodies
Serum
T-Cell Antigen Receptor Specificity
CD3 Antigens
Muromonab-CD3
Lung
Blocking Antibodies
Indirect Fluorescent Antibody Technique
T-Cell Antigen Receptor
Fluorescent Antibody Technique
Flow Cytometry

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Functional significance of anti-T-lymphocyte antibodies in sarcoidosis. / Spurzem, J. R.; Saltini, C.; Crystal, Ronald.

In: American Review of Respiratory Disease, Vol. 137, No. 3, 01.01.1988, p. 600-605.

Research output: Contribution to journalArticle

@article{22098ff4decb41639385772c311b3148,
title = "Functional significance of anti-T-lymphocyte antibodies in sarcoidosis",
abstract = "Pulmonary sarcoidosis is a chronic disorder characterized by the activation of helper/inducer T-cells in the lung without a concomitant increase in suppressor/cytotoxic T-cells. It is known that patients with sarcoidosis have circulating anti-T-cell antibodies, primarily of the IgM class. To evaluate a functional role for these antibodies in enhancing lung helper T-cell processes in pulmonary sarcoidosis, we evaluated serum and lavage fluid of patients with active sarcoidosis for the presence of anti-T-cell antibodies, the T-cell subset specificity of these antibodies, and the possible stimulatory or inhibitory effects of these antibodies on T-cells relevant to the exaggerated helper T-cell processes in sarcoidosis. Indirect immunofluorescence studies demonstrated that sarcoid patients had anti-T-cell antibodies of the IgM type reacting with autologous as well as with nonautologous normal T-cells. IgM recovered in sarcoid lavage fluid also reacted with T-cells, thus demonstrating the autoantibodies at the site of disease. Two-color immunofluorescence and flow cytometry showed that these sarcoid autoantibodies bound to mostly Leu2+ suppressor/cytotoxic T-cells, but also to a small proportion of Leu3+ helper/inducer T-cells. Incubating lymphocytes with sarcoid serum or IgM purified from sarcoid serum did not stimulate T-cell proliferation. Furthermore, when Leu2+ T-cells were stimulated with irradiated allogenic B-cells, increasing concentrations of sarcoid serum had no inhibitory effects on the activation and proliferative response of the Leu2+ T-cells. Likewise, the purified IgM anti-T-cell antibodies had no inhibitory effects on the mitogenic response of Leu2+ T-cells to the anti-T-cell antigen receptor-associated T3 complex antibody OKT3. Thus, the IgM anti-T-cell autoantibodies of sarcoidosis are present at the site of disease and bind to potentially relevant T-cell subsets; however, our study found no evidence that they have an identifiable functional role in the development of the excess helper T cell activity of sarcoidosis.",
author = "Spurzem, {J. R.} and C. Saltini and Ronald Crystal",
year = "1988",
month = "1",
day = "1",
doi = "10.1164/ajrccm/137.3.600",
language = "English",
volume = "137",
pages = "600--605",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society",
number = "3",

}

TY - JOUR

T1 - Functional significance of anti-T-lymphocyte antibodies in sarcoidosis

AU - Spurzem, J. R.

AU - Saltini, C.

AU - Crystal, Ronald

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Pulmonary sarcoidosis is a chronic disorder characterized by the activation of helper/inducer T-cells in the lung without a concomitant increase in suppressor/cytotoxic T-cells. It is known that patients with sarcoidosis have circulating anti-T-cell antibodies, primarily of the IgM class. To evaluate a functional role for these antibodies in enhancing lung helper T-cell processes in pulmonary sarcoidosis, we evaluated serum and lavage fluid of patients with active sarcoidosis for the presence of anti-T-cell antibodies, the T-cell subset specificity of these antibodies, and the possible stimulatory or inhibitory effects of these antibodies on T-cells relevant to the exaggerated helper T-cell processes in sarcoidosis. Indirect immunofluorescence studies demonstrated that sarcoid patients had anti-T-cell antibodies of the IgM type reacting with autologous as well as with nonautologous normal T-cells. IgM recovered in sarcoid lavage fluid also reacted with T-cells, thus demonstrating the autoantibodies at the site of disease. Two-color immunofluorescence and flow cytometry showed that these sarcoid autoantibodies bound to mostly Leu2+ suppressor/cytotoxic T-cells, but also to a small proportion of Leu3+ helper/inducer T-cells. Incubating lymphocytes with sarcoid serum or IgM purified from sarcoid serum did not stimulate T-cell proliferation. Furthermore, when Leu2+ T-cells were stimulated with irradiated allogenic B-cells, increasing concentrations of sarcoid serum had no inhibitory effects on the activation and proliferative response of the Leu2+ T-cells. Likewise, the purified IgM anti-T-cell antibodies had no inhibitory effects on the mitogenic response of Leu2+ T-cells to the anti-T-cell antigen receptor-associated T3 complex antibody OKT3. Thus, the IgM anti-T-cell autoantibodies of sarcoidosis are present at the site of disease and bind to potentially relevant T-cell subsets; however, our study found no evidence that they have an identifiable functional role in the development of the excess helper T cell activity of sarcoidosis.

AB - Pulmonary sarcoidosis is a chronic disorder characterized by the activation of helper/inducer T-cells in the lung without a concomitant increase in suppressor/cytotoxic T-cells. It is known that patients with sarcoidosis have circulating anti-T-cell antibodies, primarily of the IgM class. To evaluate a functional role for these antibodies in enhancing lung helper T-cell processes in pulmonary sarcoidosis, we evaluated serum and lavage fluid of patients with active sarcoidosis for the presence of anti-T-cell antibodies, the T-cell subset specificity of these antibodies, and the possible stimulatory or inhibitory effects of these antibodies on T-cells relevant to the exaggerated helper T-cell processes in sarcoidosis. Indirect immunofluorescence studies demonstrated that sarcoid patients had anti-T-cell antibodies of the IgM type reacting with autologous as well as with nonautologous normal T-cells. IgM recovered in sarcoid lavage fluid also reacted with T-cells, thus demonstrating the autoantibodies at the site of disease. Two-color immunofluorescence and flow cytometry showed that these sarcoid autoantibodies bound to mostly Leu2+ suppressor/cytotoxic T-cells, but also to a small proportion of Leu3+ helper/inducer T-cells. Incubating lymphocytes with sarcoid serum or IgM purified from sarcoid serum did not stimulate T-cell proliferation. Furthermore, when Leu2+ T-cells were stimulated with irradiated allogenic B-cells, increasing concentrations of sarcoid serum had no inhibitory effects on the activation and proliferative response of the Leu2+ T-cells. Likewise, the purified IgM anti-T-cell antibodies had no inhibitory effects on the mitogenic response of Leu2+ T-cells to the anti-T-cell antigen receptor-associated T3 complex antibody OKT3. Thus, the IgM anti-T-cell autoantibodies of sarcoidosis are present at the site of disease and bind to potentially relevant T-cell subsets; however, our study found no evidence that they have an identifiable functional role in the development of the excess helper T cell activity of sarcoidosis.

UR - http://www.scopus.com/inward/record.url?scp=0023902298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023902298&partnerID=8YFLogxK

U2 - 10.1164/ajrccm/137.3.600

DO - 10.1164/ajrccm/137.3.600

M3 - Article

C2 - 2964216

AN - SCOPUS:0023902298

VL - 137

SP - 600

EP - 605

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 3

ER -