Functional IL-18 promoter gene polymorphisms in Tunisian nasopharyngeal carcinoma patients

Karim Farhat, Elham Hassen, Nadia Bouzgarrou, Sallouha Gabbouj, Noureddine Bouaouina, Lotfi Chouchane

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objectives: There is growing evidence suggesting that IL-18 levels may affect individual to virus-associated neoplasia and that single nucleotide polymorphisms (SNPs) within the gene may influence its production. In this study we wanted to know whether IL-18 polymorphisms at positions -607 C/A and -137 G/A are associated with susceptibility and/or are markers of nasopharyngeal carcinoma (NPC) prognosis. Methods: Using the restriction fragment length polymerase chain reaction (RFLP-PCR), 163 Tunisian patients and 164 healthy controls were genotyped. Results: No significant association was found between each studied polymorphism and NPC. However, we noted that the -607 A allele, which is associated with lower IL-18 production, increased the risk of advanced tumor stages (OR = 3.59; P = 0.017) and that this risk was more pronounced among the older patient's age at onset (OR = 3.85; P = 0.012). Moreover, the significant difference in CA/GG haplotype frequency distribution between young and older patients supported the idea that NPC disease has biologically different features between age sub-groups. Conclusion: Functional IL-18 gene polymorphisms do not influence the susceptibility to NPC in Tunisians but may contribute to disease onset and aggressiveness.

Original languageEnglish
Pages (from-to)132-137
Number of pages6
JournalCytokine
Volume43
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Interleukin-18
Polymorphism
Genes
Nasopharyngeal Diseases
Polymerase Chain Reaction
Polymerase chain reaction
Viruses
Age of Onset
Restriction Fragment Length Polymorphisms
Haplotypes
Single Nucleotide Polymorphism
Tumors
Neoplasms
Nucleotides
Age Groups
Alleles
Nasopharyngeal carcinoma

Keywords

  • Interleukin-18
  • Nasopharyngeal carcinoma
  • Susceptibility

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Functional IL-18 promoter gene polymorphisms in Tunisian nasopharyngeal carcinoma patients. / Farhat, Karim; Hassen, Elham; Bouzgarrou, Nadia; Gabbouj, Sallouha; Bouaouina, Noureddine; Chouchane, Lotfi.

In: Cytokine, Vol. 43, No. 2, 08.2008, p. 132-137.

Research output: Contribution to journalArticle

Farhat, Karim ; Hassen, Elham ; Bouzgarrou, Nadia ; Gabbouj, Sallouha ; Bouaouina, Noureddine ; Chouchane, Lotfi. / Functional IL-18 promoter gene polymorphisms in Tunisian nasopharyngeal carcinoma patients. In: Cytokine. 2008 ; Vol. 43, No. 2. pp. 132-137.
@article{7f96ba10ae8842978af9db1f0f7b520f,
title = "Functional IL-18 promoter gene polymorphisms in Tunisian nasopharyngeal carcinoma patients",
abstract = "Objectives: There is growing evidence suggesting that IL-18 levels may affect individual to virus-associated neoplasia and that single nucleotide polymorphisms (SNPs) within the gene may influence its production. In this study we wanted to know whether IL-18 polymorphisms at positions -607 C/A and -137 G/A are associated with susceptibility and/or are markers of nasopharyngeal carcinoma (NPC) prognosis. Methods: Using the restriction fragment length polymerase chain reaction (RFLP-PCR), 163 Tunisian patients and 164 healthy controls were genotyped. Results: No significant association was found between each studied polymorphism and NPC. However, we noted that the -607 A allele, which is associated with lower IL-18 production, increased the risk of advanced tumor stages (OR = 3.59; P = 0.017) and that this risk was more pronounced among the older patient's age at onset (OR = 3.85; P = 0.012). Moreover, the significant difference in CA/GG haplotype frequency distribution between young and older patients supported the idea that NPC disease has biologically different features between age sub-groups. Conclusion: Functional IL-18 gene polymorphisms do not influence the susceptibility to NPC in Tunisians but may contribute to disease onset and aggressiveness.",
keywords = "Interleukin-18, Nasopharyngeal carcinoma, Susceptibility",
author = "Karim Farhat and Elham Hassen and Nadia Bouzgarrou and Sallouha Gabbouj and Noureddine Bouaouina and Lotfi Chouchane",
year = "2008",
month = "8",
doi = "10.1016/j.cyto.2008.05.004",
language = "English",
volume = "43",
pages = "132--137",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Functional IL-18 promoter gene polymorphisms in Tunisian nasopharyngeal carcinoma patients

AU - Farhat, Karim

AU - Hassen, Elham

AU - Bouzgarrou, Nadia

AU - Gabbouj, Sallouha

AU - Bouaouina, Noureddine

AU - Chouchane, Lotfi

PY - 2008/8

Y1 - 2008/8

N2 - Objectives: There is growing evidence suggesting that IL-18 levels may affect individual to virus-associated neoplasia and that single nucleotide polymorphisms (SNPs) within the gene may influence its production. In this study we wanted to know whether IL-18 polymorphisms at positions -607 C/A and -137 G/A are associated with susceptibility and/or are markers of nasopharyngeal carcinoma (NPC) prognosis. Methods: Using the restriction fragment length polymerase chain reaction (RFLP-PCR), 163 Tunisian patients and 164 healthy controls were genotyped. Results: No significant association was found between each studied polymorphism and NPC. However, we noted that the -607 A allele, which is associated with lower IL-18 production, increased the risk of advanced tumor stages (OR = 3.59; P = 0.017) and that this risk was more pronounced among the older patient's age at onset (OR = 3.85; P = 0.012). Moreover, the significant difference in CA/GG haplotype frequency distribution between young and older patients supported the idea that NPC disease has biologically different features between age sub-groups. Conclusion: Functional IL-18 gene polymorphisms do not influence the susceptibility to NPC in Tunisians but may contribute to disease onset and aggressiveness.

AB - Objectives: There is growing evidence suggesting that IL-18 levels may affect individual to virus-associated neoplasia and that single nucleotide polymorphisms (SNPs) within the gene may influence its production. In this study we wanted to know whether IL-18 polymorphisms at positions -607 C/A and -137 G/A are associated with susceptibility and/or are markers of nasopharyngeal carcinoma (NPC) prognosis. Methods: Using the restriction fragment length polymerase chain reaction (RFLP-PCR), 163 Tunisian patients and 164 healthy controls were genotyped. Results: No significant association was found between each studied polymorphism and NPC. However, we noted that the -607 A allele, which is associated with lower IL-18 production, increased the risk of advanced tumor stages (OR = 3.59; P = 0.017) and that this risk was more pronounced among the older patient's age at onset (OR = 3.85; P = 0.012). Moreover, the significant difference in CA/GG haplotype frequency distribution between young and older patients supported the idea that NPC disease has biologically different features between age sub-groups. Conclusion: Functional IL-18 gene polymorphisms do not influence the susceptibility to NPC in Tunisians but may contribute to disease onset and aggressiveness.

KW - Interleukin-18

KW - Nasopharyngeal carcinoma

KW - Susceptibility

UR - http://www.scopus.com/inward/record.url?scp=48049103897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48049103897&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2008.05.004

DO - 10.1016/j.cyto.2008.05.004

M3 - Article

VL - 43

SP - 132

EP - 137

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 2

ER -