Functional and phenotypic changes of natural killer cells in whole blood during Mycobacterium tuberculosis infection and disease

Mathieu Garand, Martin Goodier, Olumuyiwa Owolabi, Simon Donkor, Beate Kampmann, Jayne S. Sutherland

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Tuberculosis (TB) is still a global health concern, especially in resource-poor countries such as The Gambia. Defining protective immunity to TB is challenging: its pathogenesis is complex and involves several cellular components of the immune system. Recent works in vaccine development suggest important roles of the innate immunity in natural protection to TB, including natural killer (NK) cells. NK cells mediate cellular cytotoxicity and cytokine signaling in response to Mycobacterium tuberculosis (Mtb). NK cells can display specific memory-type markers to previous antigen exposure; thus, bridging innate and adaptive immunity. However, major knowledge gaps exist on the contribution of NK cells in protection against Mtb infection or TB. We performed a cross-sectional assessment of NK cells phenotype and function in four distinct groups of individuals: TB cases pre-treatment (n = 20) and post-treatment (n = 19), and household contacts with positive (n = 9) or negative (n = 18) tuberculin skin test (TST). While NK cells frequencies were similar between all groups, significant decreases in interferon-γ expression and degranulation were observed in NK cells from TB cases pre-treatment compared to post-treatment. Conversely, CD57 expression, a marker of advanced NK cells differentiation, was significantly lower in cases post-treatment compared to pre-treatment. Finally, NKG2C, an activation and imprinted-NK memory marker, was significantly increased in TST+ (latently infected) compared to TB cases pre-treatment and TST- (uninfected) individuals. The results of this study provide valuable insights into the role of NK cells in Mtb infection and TB disease, demonstrating potential markers for distinguishing between infection states and monitoring of TB treatment response.

Original languageEnglish
Article number257
JournalFrontiers in Immunology
Volume9
Issue numberFEB
DOIs
Publication statusPublished - 19 Feb 2018
Externally publishedYes

Fingerprint

Mycobacterium Infections
Mycobacterium tuberculosis
Natural Killer Cells
Tuberculosis
Tuberculin Test
Skin Tests
Innate Immunity
Gambia
Cytoprotection
Adaptive Immunity
Interferons
Cell Differentiation
Immune System
Immunity
Vaccines
Cytokines
Phenotype
Antigens

Keywords

  • CD107a
  • CD57
  • Fc gamma receptor IIIa
  • Flow cytometry
  • Innate memory
  • Interferon gamma
  • Natural killer cells
  • NKG2C

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Functional and phenotypic changes of natural killer cells in whole blood during Mycobacterium tuberculosis infection and disease. / Garand, Mathieu; Goodier, Martin; Owolabi, Olumuyiwa; Donkor, Simon; Kampmann, Beate; Sutherland, Jayne S.

In: Frontiers in Immunology, Vol. 9, No. FEB, 257, 19.02.2018.

Research output: Contribution to journalArticle

Garand, Mathieu ; Goodier, Martin ; Owolabi, Olumuyiwa ; Donkor, Simon ; Kampmann, Beate ; Sutherland, Jayne S. / Functional and phenotypic changes of natural killer cells in whole blood during Mycobacterium tuberculosis infection and disease. In: Frontiers in Immunology. 2018 ; Vol. 9, No. FEB.
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