Functional analysis of antigen-specific T lymphocytes by serial measurement of gene expression in peripheral blood mononuclear cells and tumor specimens

Udai S. Kammula, Kang Hun Lee, Adam I. Riker, Ena Wang, Galen A. Ohnmacht, Steven A. Rosenberg, Francesco M. Marincola

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Abstract

The cloning of cancer Ags recognized by T cells has provided potentially new tools to enhance immunity against metastatic cancer. The biological monitoring of effective immunization has, however, remained a dilemma. We describe here a sensitive molecular quantitation methodology that allows analysis of in vivo immune response to vaccination. Metastatic melanoma patients were immunized with a synthetically modified peptide epitope (209- 2M) from the melanoma self-Ag gp100. Using serial gene expression analysis, we report functional evidence of vaccine-induced CTL reactivity in fresh cells obtained directly from the peripheral blood of postimmunized patients. Further, we demonstrate in vivo localization of vaccine-induced immune response within the tumor microenvironment. The results of these molecular assays provide direct evidence that peptide immunization in humans can result in tumor-specific CTL that localize to metastatic sites.

Original languageEnglish
Pages (from-to)6867-6875
Number of pages9
JournalJournal of Immunology
Volume163
Issue number12
Publication statusPublished - 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Kammula, U. S., Lee, K. H., Riker, A. I., Wang, E., Ohnmacht, G. A., Rosenberg, S. A., & Marincola, F. M. (1999). Functional analysis of antigen-specific T lymphocytes by serial measurement of gene expression in peripheral blood mononuclear cells and tumor specimens. Journal of Immunology, 163(12), 6867-6875.