Fully automated organ bud detection and segmentation for Laser Capture Microdissection applications

Vincenzo Paduano, Maria Teresa De Angelis, Geppino Falco, Michele Ceccarelli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laser Capture Microdissection is a technique capable of isolating and extracting specific groups of cells of interest from microscopic regions in tissue samples. The final extraction result relies completely on the operator's ability to discriminate the tissue of interest, a very difficult task in the case of an embryo organ bud which doesn't even resemble the future organ. In this work we present a fully automated approach for localization of organ buds into embryos and segmentation of the corresponding tissue using topological, textural and morphological features processed by a Machine Learning approaches, and that can be generalized for any desired bud, species, developmental stage and preparation.

Original languageEnglish
Title of host publication2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Proceedings
Pages118-123
Number of pages6
DOIs
Publication statusPublished - 2 Sep 2011
Externally publishedYes
Event2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Batu Ferringhi, Penang, Malaysia
Duration: 17 May 201118 May 2011

Other

Other2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011
CountryMalaysia
CityBatu Ferringhi, Penang
Period17/5/1118/5/11

Fingerprint

Microdissection
Tissue
Lasers
Learning systems

Keywords

  • bud
  • embryo
  • Gabor filter
  • Hough transform
  • Laser Capture Microdissection
  • localization
  • segmentation
  • topology

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition

Cite this

Paduano, V., De Angelis, M. T., Falco, G., & Ceccarelli, M. (2011). Fully automated organ bud detection and segmentation for Laser Capture Microdissection applications. In 2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Proceedings (pp. 118-123). [5962211] https://doi.org/10.1109/IST.2011.5962211

Fully automated organ bud detection and segmentation for Laser Capture Microdissection applications. / Paduano, Vincenzo; De Angelis, Maria Teresa; Falco, Geppino; Ceccarelli, Michele.

2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Proceedings. 2011. p. 118-123 5962211.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Paduano, V, De Angelis, MT, Falco, G & Ceccarelli, M 2011, Fully automated organ bud detection and segmentation for Laser Capture Microdissection applications. in 2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Proceedings., 5962211, pp. 118-123, 2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011, Batu Ferringhi, Penang, Malaysia, 17/5/11. https://doi.org/10.1109/IST.2011.5962211
Paduano V, De Angelis MT, Falco G, Ceccarelli M. Fully automated organ bud detection and segmentation for Laser Capture Microdissection applications. In 2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Proceedings. 2011. p. 118-123. 5962211 https://doi.org/10.1109/IST.2011.5962211
Paduano, Vincenzo ; De Angelis, Maria Teresa ; Falco, Geppino ; Ceccarelli, Michele. / Fully automated organ bud detection and segmentation for Laser Capture Microdissection applications. 2011 IEEE International Conference on Imaging Systems and Techniques, IST 2011 - Proceedings. 2011. pp. 118-123
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