Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.

Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalJournal of Immunological Methods
Volume347
Issue number1-2
DOIs
Publication statusPublished - 15 Aug 2009
Externally publishedYes

Fingerprint

Cryopreservation
Regulatory T-Lymphocytes
Blood Cells
Freezing
Phenotype
Research

Keywords

  • Cryopreservation
  • T regulatory cell

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. / Elkord, Eyad.

In: Journal of Immunological Methods, Vol. 347, No. 1-2, 15.08.2009, p. 87-90.

Research output: Contribution to journalArticle

@article{eab2cc5df25a4812a65c81d31e770af2,
title = "Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation",
abstract = "Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.",
keywords = "Cryopreservation, T regulatory cell",
author = "Eyad Elkord",
year = "2009",
month = "8",
day = "15",
doi = "10.1016/j.jim.2009.06.001",
language = "English",
volume = "347",
pages = "87--90",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

AU - Elkord, Eyad

PY - 2009/8/15

Y1 - 2009/8/15

N2 - Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.

AB - Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.

KW - Cryopreservation

KW - T regulatory cell

UR - http://www.scopus.com/inward/record.url?scp=67650500734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650500734&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2009.06.001

DO - 10.1016/j.jim.2009.06.001

M3 - Article

C2 - 19538964

AN - SCOPUS:67650500734

VL - 347

SP - 87

EP - 90

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -