Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

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Abstract

Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.

Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalJournal of Immunological Methods
Volume347
Issue number1-2
DOIs
Publication statusPublished - 15 Aug 2009
Externally publishedYes

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Keywords

  • Cryopreservation
  • T regulatory cell

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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