FKBP51 reciprocally regulates GRα and PPARγ activation via the Akt-P38 pathway

Lance A. Stechschulte, Terry D. Hinds, Simona Ghanem, Weinian Shou, Sonia M. Najjar, Edwin R. Sanchez

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

FK506-binding protein 51 (FKBP51) is a negative regulator of glucocorticoid receptor-α (GRα), although the mechanism is unknown. We show here that FKBP51 is also a chaperone to peroxisome proliferator-activated receptor-γ (PPARγ), which is essential for activity, and uncover the mechanism underlying this differential regulation. In COS-7 cells, FKBP51 overexpression reduced GRα activity at a glucocorticoid response element-luciferase reporter, while increasing PPARγ activity at a peroxisome proliferator response element reporter. Conversely, FKBP51-deficient (knockout) (51KO) mouse embryonic fibroblasts (MEFs) showed elevated GRα but reduced PPARγ activities compared with those in wild-type MEFs. Phosphorylation is known to exert a similar pattern of reciprocal modulation of GRα and PPARγ. Knockdown of FKBP51 in 3T3-L1 preadipocytes increased phosphorylation of PPARγ at serine 112, a phospho-residue that inhibits activity. In 51KO cells, elevated phosphorylation of GRα at serines 220 and 234, phospho-residues that promote activity, was observed. Because FKBP51 is an essential chaperone to the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase, Akt signaling was investigated. Elevated Akt activation and increased activation of p38 kinase, a downstream target of Akt that phosphorylates GRα and PPARγ, were seen in 51KO MEFs, causing activation and inhibition, respectively. Inactivation of p38 with PD169316 reversed the effects of FKBP51 deficiency on GRα and PPARγ activities and reduced PPARγ phosphorylation. Last, loss of FKBP51 caused a shift of PPARγ from cytoplasm to nucleus, as previously shown for GRα. A model is proposed in which FKBP51 loss reciprocally regulates GRα and PPARγ via 2 complementary mechanisms: activation of Akt-p38-mediated phosphorylation and redistribution of the receptors to the nucleus for direct targeting by p38.

Original languageEnglish
Pages (from-to)1254-1264
Number of pages11
JournalMolecular Endocrinology
Volume28
Issue number8
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Fingerprint

Tacrolimus Binding Proteins
Peroxisome Proliferator-Activated Receptors
Glucocorticoid Receptors
Phosphorylation
Fibroblasts
Response Elements
Serine
Peroxisome Proliferators
Protein Deficiency
Phosphoprotein Phosphatases
COS Cells
Luciferases
Phosphoric Monoester Hydrolases
Knockout Mice
Glucocorticoids
Cytoplasm
Phosphotransferases

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Stechschulte, L. A., Hinds, T. D., Ghanem, S., Shou, W., Najjar, S. M., & Sanchez, E. R. (2014). FKBP51 reciprocally regulates GRα and PPARγ activation via the Akt-P38 pathway. Molecular Endocrinology, 28(8), 1254-1264. https://doi.org/10.1210/me.2014-1023

FKBP51 reciprocally regulates GRα and PPARγ activation via the Akt-P38 pathway. / Stechschulte, Lance A.; Hinds, Terry D.; Ghanem, Simona; Shou, Weinian; Najjar, Sonia M.; Sanchez, Edwin R.

In: Molecular Endocrinology, Vol. 28, No. 8, 01.01.2014, p. 1254-1264.

Research output: Contribution to journalArticle

Stechschulte, LA, Hinds, TD, Ghanem, S, Shou, W, Najjar, SM & Sanchez, ER 2014, 'FKBP51 reciprocally regulates GRα and PPARγ activation via the Akt-P38 pathway', Molecular Endocrinology, vol. 28, no. 8, pp. 1254-1264. https://doi.org/10.1210/me.2014-1023
Stechschulte, Lance A. ; Hinds, Terry D. ; Ghanem, Simona ; Shou, Weinian ; Najjar, Sonia M. ; Sanchez, Edwin R. / FKBP51 reciprocally regulates GRα and PPARγ activation via the Akt-P38 pathway. In: Molecular Endocrinology. 2014 ; Vol. 28, No. 8. pp. 1254-1264.
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