Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes

Darawan Rinchai, Esperanza Anguiano, Phuong Nguyen, Damien J. Chaussabel

Research output: Contribution to journalComment/debate

1 Citation (Scopus)

Abstract

With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

Original languageEnglish
Article number1385
JournalF1000Research
Volume5
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Gene Expression Profiling
Gene expression
Fingers
Blood
RNA
Blood Volume
RNA Sequence Analysis
Peer Review
Publications
Microarrays
Real-Time Polymerase Chain Reaction
Pharmacology
Technology
Stabilization
Health
Monitoring
Pharmaceutical Preparations

Keywords

  • Blood collection
  • Fingerstick
  • Gene expression
  • RNA
  • Tempus
  • Transcriptome

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes. / Rinchai, Darawan; Anguiano, Esperanza; Nguyen, Phuong; Chaussabel, Damien J.

In: F1000Research, Vol. 5, 1385, 2017.

Research output: Contribution to journalComment/debate

@article{503e56a0aca24d39a52e1617a49980e6,
title = "Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes",
abstract = "With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.",
keywords = "Blood collection, Fingerstick, Gene expression, RNA, Tempus, Transcriptome",
author = "Darawan Rinchai and Esperanza Anguiano and Phuong Nguyen and Chaussabel, {Damien J.}",
year = "2017",
doi = "10.12688/f1000research.8841.2",
language = "English",
volume = "5",
journal = "F1000Research",
issn = "2046-1402",
publisher = "F1000 Research Ltd.",

}

TY - JOUR

T1 - Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes

AU - Rinchai, Darawan

AU - Anguiano, Esperanza

AU - Nguyen, Phuong

AU - Chaussabel, Damien J.

PY - 2017

Y1 - 2017

N2 - With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

AB - With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

KW - Blood collection

KW - Fingerstick

KW - Gene expression

KW - RNA

KW - Tempus

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85017091370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85017091370&partnerID=8YFLogxK

U2 - 10.12688/f1000research.8841.2

DO - 10.12688/f1000research.8841.2

M3 - Comment/debate

VL - 5

JO - F1000Research

JF - F1000Research

SN - 2046-1402

M1 - 1385

ER -