FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC

John Lalith Charles Richard, Manu Shubhdarshan Shukla, Hervé Menoni, Khalid Ouararhni, Imtiaz Nisar Lone, Yohan Roulland, Christophe Papin, Elsa Ben Simon, Tapas Kundu, Ali Hamiche, Dimitar Angelov, Stefan Dimitrov

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.

Original languageEnglish
Article numbere1006221
JournalPLoS Genetics
Volume12
Issue number7
DOIs
Publication statusPublished - 1 Jul 2016
Externally publishedYes

Fingerprint

DNA repair
DNA Repair
repair
Chromatin Assembly and Disassembly
chromatin
DNA
transcription (genetics)
Uracil-DNA Glycosidase
Uracil
Double-Stranded DNA Breaks
glycosylases
uracil
Chromatin
Proteins
Oxidative Stress
protein
oxidative stress
proteins
lesion
damage

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Charles Richard, J. L., Shukla, M. S., Menoni, H., Ouararhni, K., Lone, I. N., Roulland, Y., ... Dimitrov, S. (2016). FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. PLoS Genetics, 12(7), [e1006221]. https://doi.org/10.1371/journal.pgen.1006221

FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. / Charles Richard, John Lalith; Shukla, Manu Shubhdarshan; Menoni, Hervé; Ouararhni, Khalid; Lone, Imtiaz Nisar; Roulland, Yohan; Papin, Christophe; Ben Simon, Elsa; Kundu, Tapas; Hamiche, Ali; Angelov, Dimitar; Dimitrov, Stefan.

In: PLoS Genetics, Vol. 12, No. 7, e1006221, 01.07.2016.

Research output: Contribution to journalArticle

Charles Richard, JL, Shukla, MS, Menoni, H, Ouararhni, K, Lone, IN, Roulland, Y, Papin, C, Ben Simon, E, Kundu, T, Hamiche, A, Angelov, D & Dimitrov, S 2016, 'FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC', PLoS Genetics, vol. 12, no. 7, e1006221. https://doi.org/10.1371/journal.pgen.1006221
Charles Richard JL, Shukla MS, Menoni H, Ouararhni K, Lone IN, Roulland Y et al. FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. PLoS Genetics. 2016 Jul 1;12(7). e1006221. https://doi.org/10.1371/journal.pgen.1006221
Charles Richard, John Lalith ; Shukla, Manu Shubhdarshan ; Menoni, Hervé ; Ouararhni, Khalid ; Lone, Imtiaz Nisar ; Roulland, Yohan ; Papin, Christophe ; Ben Simon, Elsa ; Kundu, Tapas ; Hamiche, Ali ; Angelov, Dimitar ; Dimitrov, Stefan. / FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. In: PLoS Genetics. 2016 ; Vol. 12, No. 7.
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