Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering

Francesca Colazzo, Padmini Sarathchandra, Ryszard T. Smolenski, Adrian H. Chester, Yuan Tsan Tseng, Jan T. Czernuszka, Magdi H. Yacoub, Patricia M. Taylor

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A key challenge in tissue engineering a heart valve is to reproduce the major tissue structures responsible for native valve function. Here we evaluated human adipose-derived stem cells (ADSCs) as a source of cells for heart valve tissue engineering investigating their ability to synthesize and process collagen and elastin. ADSCs were compared with human bone marrow mesenchymal stem cells (BmMSCs) and human aortic valve interstitial cells (hVICs). ADSCs and BmMSCs were stretched at 14% for 3 days and collagen synthesis determined by [3H]-proline incorporation. Collagen and elastin crosslinking was assessed by measuring pyridinoline and desmosine respectively, using liquid chromatography/mass spectrometry. Three-dimensional culture was obtained by seeding cells onto bovine collagen type I scaffolds for 2-20 days. Expression of matrix proteins and processing enzymes was assessed by Real Time-PCR, immunofluorescence and transmission electron microscopy. Stretch increased the incorporation of [3H]-proline in ADSCs and BmMSCs, however only ADSCs and hVICs upregulated COL3A1 gene. ADSCs produced collagen and elastin crosslinks. ADSCs uniformly populated collagen scaffolds after 2 days, and fibrillar-like collagen was detected after 20 days. ADSCs sense mechanical stimulation and produce and process collagen and elastin. These novel findings have important implications for the use of these cells in tissue engineering.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalBiomaterials
Volume32
Issue number1
DOIs
Publication statusPublished - 1 Jan 2011
Externally publishedYes

Fingerprint

Heart Valves
Tissue Engineering
Stem cells
Tissue engineering
Extracellular Matrix
Stem Cells
Collagen
Elastin
Mesenchymal Stromal Cells
Bone Marrow
Aortic Valve
Proline
Bone
Desmosine
Scaffolds (biology)
Fibrillar Collagens
Scaffolds
Collagen Type I
Transmission Electron Microscopy
Liquid Chromatography

Keywords

  • Adipose-derived stem cells
  • Collagen
  • Crosslinks
  • Elastin
  • Extracellular matrix
  • Heart valve

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Colazzo, F., Sarathchandra, P., Smolenski, R. T., Chester, A. H., Tseng, Y. T., Czernuszka, J. T., ... Taylor, P. M. (2011). Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering. Biomaterials, 32(1), 119-127. https://doi.org/10.1016/j.biomaterials.2010.09.003

Extracellular matrix production by adipose-derived stem cells : Implications for heart valve tissue engineering. / Colazzo, Francesca; Sarathchandra, Padmini; Smolenski, Ryszard T.; Chester, Adrian H.; Tseng, Yuan Tsan; Czernuszka, Jan T.; Yacoub, Magdi H.; Taylor, Patricia M.

In: Biomaterials, Vol. 32, No. 1, 01.01.2011, p. 119-127.

Research output: Contribution to journalArticle

Colazzo, F, Sarathchandra, P, Smolenski, RT, Chester, AH, Tseng, YT, Czernuszka, JT, Yacoub, MH & Taylor, PM 2011, 'Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering', Biomaterials, vol. 32, no. 1, pp. 119-127. https://doi.org/10.1016/j.biomaterials.2010.09.003
Colazzo F, Sarathchandra P, Smolenski RT, Chester AH, Tseng YT, Czernuszka JT et al. Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering. Biomaterials. 2011 Jan 1;32(1):119-127. https://doi.org/10.1016/j.biomaterials.2010.09.003
Colazzo, Francesca ; Sarathchandra, Padmini ; Smolenski, Ryszard T. ; Chester, Adrian H. ; Tseng, Yuan Tsan ; Czernuszka, Jan T. ; Yacoub, Magdi H. ; Taylor, Patricia M. / Extracellular matrix production by adipose-derived stem cells : Implications for heart valve tissue engineering. In: Biomaterials. 2011 ; Vol. 32, No. 1. pp. 119-127.
@article{20e3f8723bab44328411dc88ed89358a,
title = "Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering",
abstract = "A key challenge in tissue engineering a heart valve is to reproduce the major tissue structures responsible for native valve function. Here we evaluated human adipose-derived stem cells (ADSCs) as a source of cells for heart valve tissue engineering investigating their ability to synthesize and process collagen and elastin. ADSCs were compared with human bone marrow mesenchymal stem cells (BmMSCs) and human aortic valve interstitial cells (hVICs). ADSCs and BmMSCs were stretched at 14{\%} for 3 days and collagen synthesis determined by [3H]-proline incorporation. Collagen and elastin crosslinking was assessed by measuring pyridinoline and desmosine respectively, using liquid chromatography/mass spectrometry. Three-dimensional culture was obtained by seeding cells onto bovine collagen type I scaffolds for 2-20 days. Expression of matrix proteins and processing enzymes was assessed by Real Time-PCR, immunofluorescence and transmission electron microscopy. Stretch increased the incorporation of [3H]-proline in ADSCs and BmMSCs, however only ADSCs and hVICs upregulated COL3A1 gene. ADSCs produced collagen and elastin crosslinks. ADSCs uniformly populated collagen scaffolds after 2 days, and fibrillar-like collagen was detected after 20 days. ADSCs sense mechanical stimulation and produce and process collagen and elastin. These novel findings have important implications for the use of these cells in tissue engineering.",
keywords = "Adipose-derived stem cells, Collagen, Crosslinks, Elastin, Extracellular matrix, Heart valve",
author = "Francesca Colazzo and Padmini Sarathchandra and Smolenski, {Ryszard T.} and Chester, {Adrian H.} and Tseng, {Yuan Tsan} and Czernuszka, {Jan T.} and Yacoub, {Magdi H.} and Taylor, {Patricia M.}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.biomaterials.2010.09.003",
language = "English",
volume = "32",
pages = "119--127",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Extracellular matrix production by adipose-derived stem cells

T2 - Implications for heart valve tissue engineering

AU - Colazzo, Francesca

AU - Sarathchandra, Padmini

AU - Smolenski, Ryszard T.

AU - Chester, Adrian H.

AU - Tseng, Yuan Tsan

AU - Czernuszka, Jan T.

AU - Yacoub, Magdi H.

AU - Taylor, Patricia M.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - A key challenge in tissue engineering a heart valve is to reproduce the major tissue structures responsible for native valve function. Here we evaluated human adipose-derived stem cells (ADSCs) as a source of cells for heart valve tissue engineering investigating their ability to synthesize and process collagen and elastin. ADSCs were compared with human bone marrow mesenchymal stem cells (BmMSCs) and human aortic valve interstitial cells (hVICs). ADSCs and BmMSCs were stretched at 14% for 3 days and collagen synthesis determined by [3H]-proline incorporation. Collagen and elastin crosslinking was assessed by measuring pyridinoline and desmosine respectively, using liquid chromatography/mass spectrometry. Three-dimensional culture was obtained by seeding cells onto bovine collagen type I scaffolds for 2-20 days. Expression of matrix proteins and processing enzymes was assessed by Real Time-PCR, immunofluorescence and transmission electron microscopy. Stretch increased the incorporation of [3H]-proline in ADSCs and BmMSCs, however only ADSCs and hVICs upregulated COL3A1 gene. ADSCs produced collagen and elastin crosslinks. ADSCs uniformly populated collagen scaffolds after 2 days, and fibrillar-like collagen was detected after 20 days. ADSCs sense mechanical stimulation and produce and process collagen and elastin. These novel findings have important implications for the use of these cells in tissue engineering.

AB - A key challenge in tissue engineering a heart valve is to reproduce the major tissue structures responsible for native valve function. Here we evaluated human adipose-derived stem cells (ADSCs) as a source of cells for heart valve tissue engineering investigating their ability to synthesize and process collagen and elastin. ADSCs were compared with human bone marrow mesenchymal stem cells (BmMSCs) and human aortic valve interstitial cells (hVICs). ADSCs and BmMSCs were stretched at 14% for 3 days and collagen synthesis determined by [3H]-proline incorporation. Collagen and elastin crosslinking was assessed by measuring pyridinoline and desmosine respectively, using liquid chromatography/mass spectrometry. Three-dimensional culture was obtained by seeding cells onto bovine collagen type I scaffolds for 2-20 days. Expression of matrix proteins and processing enzymes was assessed by Real Time-PCR, immunofluorescence and transmission electron microscopy. Stretch increased the incorporation of [3H]-proline in ADSCs and BmMSCs, however only ADSCs and hVICs upregulated COL3A1 gene. ADSCs produced collagen and elastin crosslinks. ADSCs uniformly populated collagen scaffolds after 2 days, and fibrillar-like collagen was detected after 20 days. ADSCs sense mechanical stimulation and produce and process collagen and elastin. These novel findings have important implications for the use of these cells in tissue engineering.

KW - Adipose-derived stem cells

KW - Collagen

KW - Crosslinks

KW - Elastin

KW - Extracellular matrix

KW - Heart valve

UR - http://www.scopus.com/inward/record.url?scp=78349304182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78349304182&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2010.09.003

DO - 10.1016/j.biomaterials.2010.09.003

M3 - Article

C2 - 21074262

AN - SCOPUS:78349304182

VL - 32

SP - 119

EP - 127

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 1

ER -