Extra views on structure and dynamics of DNA loops on nucleosomes studied with molecular simulations

Marco Pasi, Dimitar Angelov, Jan Bednar, Stefan Dimitrov, Richard Lavery

Research output: Contribution to journalReview article

Abstract

It has been shown experimentally that the action of the RSC chromatin remodeler leads to the formation of an irregular, partially remodeled nucleosome, termed a remosome. The remosome contains an extra 30–40 base pairs of DNA compared to a canonical nucleosome. Large-scale molecular simulations have provided information on the probable structure of remosomes and have explained why they remain stable in the absence of RSC. Here we explain how these simulations were carried out and what the resulting remosome models imply in terms of the mechanism of action of RSC. We notably show that local kinks within DNA are key in explaining how extra DNA can be in added to nucleosomes without unduly disturbing DNA-histone binding.

Original languageEnglish
Pages (from-to)554-559
Number of pages6
JournalNucleus
Volume7
Issue number6
DOIs
Publication statusPublished - 1 Nov 2016
Externally publishedYes

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Keywords

  • chromatin remodeling
  • DNA
  • DNA kinks
  • DNA loops
  • molecular dynamics
  • nucleosome
  • remosome
  • RSC
  • SWI/SNF

ASJC Scopus subject areas

  • Cell Biology

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