External glycopeptide binding to MHC class-I in relation to expression of TAP transporters, β2-microglobulin and to pH

Ussama M. Abdel-Motal, Jan Dahmén, Liu Tianmin, Hans Gustaf Ljunggren, Mikael Jondal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

MHC class-I binding glycopeptides are easily visualized on the cell surface by carbohydrate specific monoclonal antibodies. By comparing the staining intensity between anti-carbohydrate and anti-MHC class-I specific monoclonal antibodies, an estimation of the fraction of peptide accessible 'empty' sites on the cell surface of MHC class-1 molecules can be made. This system was used to analyze glycopeptide binding to MHC class-I molecules in relation to transporter associated with antigen processing (TAP) peptide transporters and β2-M expression, using gene targeted mice, and in relation to pH. Approximately 15, 40, and 95% 'empty' D(b) molecules were found on activated T cells from normal, β2-M-/- and TAP -/- mice, respectively. The ASN9-6h-Gal2 glycopeptide also bound to transfected 'empty' D(b) molecules on T1-D(b), T2-D(b) and T3-D(b) cells with a preference for T2-D(b) cells, lacking TAP peptide transporters. The stability of glycopeptide binding to H-2D(b) is also highest on T2-D(b) cells. pH was found to influence binding either positively or negatively, using four different glycopeptides, binding either to D(b) or K(b). We conclude that external glycopeptide binding may reflect important functional properties in the MHC class-I system and that pH in different processing compartments might influence the expressed peptide repertoire.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalImmunology Letters
Volume54
Issue number1
DOIs
Publication statusPublished - 1 Dec 1996
Externally publishedYes

Fingerprint

Glycopeptides
trypsinogen activation peptide
Monoclonal Antibodies
Carbohydrates
Peptides
transporter associated with antigen processing (TAP)
Staining and Labeling
T-Lymphocytes
Gene Expression

Keywords

  • endosomes
  • MHC class-I
  • peptide
  • pH
  • transporter associated with antigen processing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

External glycopeptide binding to MHC class-I in relation to expression of TAP transporters, β2-microglobulin and to pH. / Abdel-Motal, Ussama M.; Dahmén, Jan; Tianmin, Liu; Ljunggren, Hans Gustaf; Jondal, Mikael.

In: Immunology Letters, Vol. 54, No. 1, 01.12.1996, p. 31-35.

Research output: Contribution to journalArticle

Abdel-Motal, Ussama M. ; Dahmén, Jan ; Tianmin, Liu ; Ljunggren, Hans Gustaf ; Jondal, Mikael. / External glycopeptide binding to MHC class-I in relation to expression of TAP transporters, β2-microglobulin and to pH. In: Immunology Letters. 1996 ; Vol. 54, No. 1. pp. 31-35.
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