Expression profiling and identification of novel genes involved in myogenic differentiation.

Kinga K. Tomczak, Voichita D. Marinescu, Marco F. Ramoni, Despina Sanoudou, Federica Montanaro, Mei Han, Louis M. Kunkel, Isaac S. Kohane, Alan H. Beggs

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Skeletal muscle differentiation is a complex, highly coordinated process that relies on precise temporal gene expression patterns. To better understand this cascade of transcriptional events, we used expression profiling to analyze gene expression in a 12-day time course of differentiating C2C12 myoblasts. Cluster analysis specific for time-ordered microarray experiments classified 2895 genes and ESTs with variable expression levels between proliferating and differentiating cells into 22 clusters with distinct expression patterns during myogenesis. Expression patterns for several known and novel genes were independently confirmed by real-time quantitative RT-PCR and/or Western blotting and immunofluorescence. MyoD and MEF family members exhibited unique expression kinetics that were highly coordinated with cell-cycle withdrawal regulators. Among genes with peak expression levels during cell cycle withdrawal were Vcam1, Itgb3, Itga5, Vcl, as well as Ptger4, a gene not previously associated with the process of myogenesis. One interesting uncharacterized transcript that is highly induced during myogenesis encodes several immunoglobulin repeats with sequence similarity to titin, a large sarcomeric protein. These data sets identify many additional uncharacterized transcripts that may play important functions in muscle cell proliferation and differentiation and provide a baseline for comparison with C2C12 cells expressing various mutant genes involved in myopathic disorders.

Original languageEnglish
Pages (from-to)403-405
Number of pages3
JournalThe FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume18
Issue number2
Publication statusPublished - 1 Feb 2004
Externally publishedYes

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Genes
Muscle Development
Cells
Gene expression
Muscle
Cell Cycle
Connectin
Gene Expression
Myoblasts
Expressed Sequence Tags
Cell proliferation
Cluster analysis
Microarrays
Muscle Cells
Fluorescent Antibody Technique
Cluster Analysis
Immunoglobulins
Real-Time Polymerase Chain Reaction
Cell Differentiation
Skeletal Muscle

Cite this

Expression profiling and identification of novel genes involved in myogenic differentiation. / Tomczak, Kinga K.; Marinescu, Voichita D.; Ramoni, Marco F.; Sanoudou, Despina; Montanaro, Federica; Han, Mei; Kunkel, Louis M.; Kohane, Isaac S.; Beggs, Alan H.

In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, No. 2, 01.02.2004, p. 403-405.

Research output: Contribution to journalArticle

Tomczak, KK, Marinescu, VD, Ramoni, MF, Sanoudou, D, Montanaro, F, Han, M, Kunkel, LM, Kohane, IS & Beggs, AH 2004, 'Expression profiling and identification of novel genes involved in myogenic differentiation.', The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 18, no. 2, pp. 403-405.
Tomczak, Kinga K. ; Marinescu, Voichita D. ; Ramoni, Marco F. ; Sanoudou, Despina ; Montanaro, Federica ; Han, Mei ; Kunkel, Louis M. ; Kohane, Isaac S. ; Beggs, Alan H. / Expression profiling and identification of novel genes involved in myogenic differentiation. In: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2004 ; Vol. 18, No. 2. pp. 403-405.
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