Expression profiles of the connexin genes, Gjb1 and Gjb3, in the developing mouse cochlea

Núria López-Bigas, Maria L. Arbonés, Xavier P. Estivill, Lionel Simounneau

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Several connexin genes (GJB1, GJB2, GJB3, GJB6 and GJA1) have been found mutated in patients with non-syndromic and/or syndromic deafness indicating an important role of these proteins in the auditory system. In order to better understand the function of the connexins in the inner ear we have analyzed the gene expression profiles of two connexin genes, Gjb1 (connexin 32) and Gjb3 (connexin 31), by in situ hybridization during the mouse cochlea organogenesis, from early otocyst up to the mature organ in adult. In the developing otocyst epithelium, some restricted domains expressed Gjb3 and Gjb1 whilst high levels of both transcripts were present in the surrounding mesenchymal tissue. As development proceeds, expression of these two genes was found in various subtypes of fibrocytes, either within the spiral limbus or along the spiral ligament, as well as in the basilar membrane cells, in the Reissner's membrane cells, and in subsets of the cellular elements of the cochlear ganglion. Gjb3 and Gjb1 expression was spatiotemporally modulated within the sensory hair cells and the various supporting cells that compose the developing organ of Corti. A transitory expression of Gjb1 was found in the basal and intermediate cells of the stria vascularis. In the adult cochlea Gjb1 transcripts disappeared while Gjb3 expression remained present in fibrocytes with specific expression patterns.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalGene Expression Patterns
Volume2
Issue number1-2
DOIs
Publication statusPublished - Nov 2002
Externally publishedYes

Fingerprint

Connexins
Cochlea
Transcriptome
Spiral Ligament of Cochlea
Basilar Membrane
Stria Vascularis
Organ of Corti
Organogenesis
Deafness
Inner Ear
Ganglia
Genes
In Situ Hybridization
Epithelium
Cell Membrane
Gene Expression
Proteins

Keywords

  • Beta-connexins
  • Cochlea
  • Connexin 31
  • Connexin 31.1
  • Connexin 32
  • Deafness
  • Gap junctions
  • Gjb1
  • Gjb3
  • Gjb5
  • Hearing loss
  • Inner ear
  • Mouse development
  • Organ of Corti

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Expression profiles of the connexin genes, Gjb1 and Gjb3, in the developing mouse cochlea. / López-Bigas, Núria; Arbonés, Maria L.; Estivill, Xavier P.; Simounneau, Lionel.

In: Gene Expression Patterns, Vol. 2, No. 1-2, 11.2002, p. 113-117.

Research output: Contribution to journalArticle

López-Bigas, Núria ; Arbonés, Maria L. ; Estivill, Xavier P. ; Simounneau, Lionel. / Expression profiles of the connexin genes, Gjb1 and Gjb3, in the developing mouse cochlea. In: Gene Expression Patterns. 2002 ; Vol. 2, No. 1-2. pp. 113-117.
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