Expression of the secretory leukoprotease inhibitor gene in epithelial cells

Tatsuya Abe, Nobuyuki Kobayashi, Kunihiko Yoshimura, Bruce C. Trapnell, Haimee Kim, Richard C. Hubbard, Michael T. Brewer, Robert C. Thompson, Ronald Crystal

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

The secretory leukoprotease inhibitor (SLPI) gene codes for a 12-kD protein that within the lung protects the airway epithelium from neutrophil elastase. Screening of 228 alleles in 114 individuals for sequence differences by RNase protection of genomic DNA revealed no detectable polymorphisms in SLPI gene exons II-IV. SLPI gene expression in the lung was demonstrated by identifying SLPI mRNA transcripts in bronchial epithelial cells freshly isolated from normals. Cell lines derived from mucosal surfaces (HS-24 bronchial squamous cell carcinoma, HeLa cervical carcinoma) actively transcribe the SLPI gene and contain SLPI mRNA transcripts, while lung fibroblasts demonstrate no evidence of SLPI gene expression. SLPI mRNA transcripts appear to be relatively stable, with mRNA levels only mildly affected by inhibition of RNA synthesis. Chromatin DNA of HS-24 cells demonstrates two DNase I hypersensitivity sites within the 5′ flanking region of exon I of the SLPI gene, whereas fibroblast chromatin has no DNase I accessible sites in the same region. Further analysis of the 5′ flanking region demonstrated two contiguous transcription start sites, CAAT and TATA boxes, and several potential regions of known DNA binding proteins. Overall, the SLPI gene appears to be a relatively nonpolymorphic, stable gene that is constitutively expressed at specific tissue sites, but has the potential to be modulated at both the transcriptional and posttranscriptional levels.

Original languageEnglish
Pages (from-to)2207-2215
Number of pages9
JournalJournal of Clinical Investigation
Volume87
Issue number6
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

Fingerprint

Secretory Leukocyte Peptidase Inhibitor
Epithelial Cells
Genes
Messenger RNA
5' Flanking Region
Deoxyribonuclease I
Lung
Chromatin
Exons
Fibroblasts
Gene Expression
Leukocyte Elastase
TATA Box
Transcription Initiation Site
DNA
DNA-Binding Proteins
Ribonucleases
Individuality
Squamous Cell Carcinoma
Hypersensitivity

Keywords

  • 5′ flanking region
  • DNase I hypersensitivity site
  • mRNA transcript stability
  • Polymorphism
  • Transcription

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Abe, T., Kobayashi, N., Yoshimura, K., Trapnell, B. C., Kim, H., Hubbard, R. C., ... Crystal, R. (1991). Expression of the secretory leukoprotease inhibitor gene in epithelial cells. Journal of Clinical Investigation, 87(6), 2207-2215. https://doi.org/10.1172/JCI115255

Expression of the secretory leukoprotease inhibitor gene in epithelial cells. / Abe, Tatsuya; Kobayashi, Nobuyuki; Yoshimura, Kunihiko; Trapnell, Bruce C.; Kim, Haimee; Hubbard, Richard C.; Brewer, Michael T.; Thompson, Robert C.; Crystal, Ronald.

In: Journal of Clinical Investigation, Vol. 87, No. 6, 01.01.1991, p. 2207-2215.

Research output: Contribution to journalArticle

Abe, T, Kobayashi, N, Yoshimura, K, Trapnell, BC, Kim, H, Hubbard, RC, Brewer, MT, Thompson, RC & Crystal, R 1991, 'Expression of the secretory leukoprotease inhibitor gene in epithelial cells', Journal of Clinical Investigation, vol. 87, no. 6, pp. 2207-2215. https://doi.org/10.1172/JCI115255
Abe T, Kobayashi N, Yoshimura K, Trapnell BC, Kim H, Hubbard RC et al. Expression of the secretory leukoprotease inhibitor gene in epithelial cells. Journal of Clinical Investigation. 1991 Jan 1;87(6):2207-2215. https://doi.org/10.1172/JCI115255
Abe, Tatsuya ; Kobayashi, Nobuyuki ; Yoshimura, Kunihiko ; Trapnell, Bruce C. ; Kim, Haimee ; Hubbard, Richard C. ; Brewer, Michael T. ; Thompson, Robert C. ; Crystal, Ronald. / Expression of the secretory leukoprotease inhibitor gene in epithelial cells. In: Journal of Clinical Investigation. 1991 ; Vol. 87, No. 6. pp. 2207-2215.
@article{23c6bab82f664e29bf7597c8f1e82be4,
title = "Expression of the secretory leukoprotease inhibitor gene in epithelial cells",
abstract = "The secretory leukoprotease inhibitor (SLPI) gene codes for a 12-kD protein that within the lung protects the airway epithelium from neutrophil elastase. Screening of 228 alleles in 114 individuals for sequence differences by RNase protection of genomic DNA revealed no detectable polymorphisms in SLPI gene exons II-IV. SLPI gene expression in the lung was demonstrated by identifying SLPI mRNA transcripts in bronchial epithelial cells freshly isolated from normals. Cell lines derived from mucosal surfaces (HS-24 bronchial squamous cell carcinoma, HeLa cervical carcinoma) actively transcribe the SLPI gene and contain SLPI mRNA transcripts, while lung fibroblasts demonstrate no evidence of SLPI gene expression. SLPI mRNA transcripts appear to be relatively stable, with mRNA levels only mildly affected by inhibition of RNA synthesis. Chromatin DNA of HS-24 cells demonstrates two DNase I hypersensitivity sites within the 5′ flanking region of exon I of the SLPI gene, whereas fibroblast chromatin has no DNase I accessible sites in the same region. Further analysis of the 5′ flanking region demonstrated two contiguous transcription start sites, CAAT and TATA boxes, and several potential regions of known DNA binding proteins. Overall, the SLPI gene appears to be a relatively nonpolymorphic, stable gene that is constitutively expressed at specific tissue sites, but has the potential to be modulated at both the transcriptional and posttranscriptional levels.",
keywords = "5′ flanking region, DNase I hypersensitivity site, mRNA transcript stability, Polymorphism, Transcription",
author = "Tatsuya Abe and Nobuyuki Kobayashi and Kunihiko Yoshimura and Trapnell, {Bruce C.} and Haimee Kim and Hubbard, {Richard C.} and Brewer, {Michael T.} and Thompson, {Robert C.} and Ronald Crystal",
year = "1991",
month = "1",
day = "1",
doi = "10.1172/JCI115255",
language = "English",
volume = "87",
pages = "2207--2215",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "6",

}

TY - JOUR

T1 - Expression of the secretory leukoprotease inhibitor gene in epithelial cells

AU - Abe, Tatsuya

AU - Kobayashi, Nobuyuki

AU - Yoshimura, Kunihiko

AU - Trapnell, Bruce C.

AU - Kim, Haimee

AU - Hubbard, Richard C.

AU - Brewer, Michael T.

AU - Thompson, Robert C.

AU - Crystal, Ronald

PY - 1991/1/1

Y1 - 1991/1/1

N2 - The secretory leukoprotease inhibitor (SLPI) gene codes for a 12-kD protein that within the lung protects the airway epithelium from neutrophil elastase. Screening of 228 alleles in 114 individuals for sequence differences by RNase protection of genomic DNA revealed no detectable polymorphisms in SLPI gene exons II-IV. SLPI gene expression in the lung was demonstrated by identifying SLPI mRNA transcripts in bronchial epithelial cells freshly isolated from normals. Cell lines derived from mucosal surfaces (HS-24 bronchial squamous cell carcinoma, HeLa cervical carcinoma) actively transcribe the SLPI gene and contain SLPI mRNA transcripts, while lung fibroblasts demonstrate no evidence of SLPI gene expression. SLPI mRNA transcripts appear to be relatively stable, with mRNA levels only mildly affected by inhibition of RNA synthesis. Chromatin DNA of HS-24 cells demonstrates two DNase I hypersensitivity sites within the 5′ flanking region of exon I of the SLPI gene, whereas fibroblast chromatin has no DNase I accessible sites in the same region. Further analysis of the 5′ flanking region demonstrated two contiguous transcription start sites, CAAT and TATA boxes, and several potential regions of known DNA binding proteins. Overall, the SLPI gene appears to be a relatively nonpolymorphic, stable gene that is constitutively expressed at specific tissue sites, but has the potential to be modulated at both the transcriptional and posttranscriptional levels.

AB - The secretory leukoprotease inhibitor (SLPI) gene codes for a 12-kD protein that within the lung protects the airway epithelium from neutrophil elastase. Screening of 228 alleles in 114 individuals for sequence differences by RNase protection of genomic DNA revealed no detectable polymorphisms in SLPI gene exons II-IV. SLPI gene expression in the lung was demonstrated by identifying SLPI mRNA transcripts in bronchial epithelial cells freshly isolated from normals. Cell lines derived from mucosal surfaces (HS-24 bronchial squamous cell carcinoma, HeLa cervical carcinoma) actively transcribe the SLPI gene and contain SLPI mRNA transcripts, while lung fibroblasts demonstrate no evidence of SLPI gene expression. SLPI mRNA transcripts appear to be relatively stable, with mRNA levels only mildly affected by inhibition of RNA synthesis. Chromatin DNA of HS-24 cells demonstrates two DNase I hypersensitivity sites within the 5′ flanking region of exon I of the SLPI gene, whereas fibroblast chromatin has no DNase I accessible sites in the same region. Further analysis of the 5′ flanking region demonstrated two contiguous transcription start sites, CAAT and TATA boxes, and several potential regions of known DNA binding proteins. Overall, the SLPI gene appears to be a relatively nonpolymorphic, stable gene that is constitutively expressed at specific tissue sites, but has the potential to be modulated at both the transcriptional and posttranscriptional levels.

KW - 5′ flanking region

KW - DNase I hypersensitivity site

KW - mRNA transcript stability

KW - Polymorphism

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0026042309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026042309&partnerID=8YFLogxK

U2 - 10.1172/JCI115255

DO - 10.1172/JCI115255

M3 - Article

VL - 87

SP - 2207

EP - 2215

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 6

ER -