Expression of the neutrophil elastase gene during human bone marrow cell differentiation

P. Fouret, R. M. Du Bois, J. F. Bernaudin, H. Takahashi, V. J. Ferrans, Ronald Crystal

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Neutrophil elastase, a potent serine protease carried and released by activated neutrophils, is not synthesized by neutrophils, but by their bone marrow precursor cells. Using in situ hybridization with 35S-labeled antisense and sense neutrophil elastase cRNA probes, the present study demonstrates that expression of the neutrophil elastase gene is tightly controlled in bone marrow precursors and occurs during a very limited stage of differentiation of the neutrophil myeloid series, almost entirely at the promyelocyte stage. Neutrophil elastase mRNA transcript levels are detectable to a limited extent in blasts, increase markedly in the promyelocyte stage, and then disappear as promyelocytes further differentiate. Control probes specific for myeloperoxidase, lactoferrin, and β-globin mRNA transcripts, respectively, demonstrated contrasting gene expression. Myeloperoxidase mRNA transcripts were also found almost exclusively at the promyelocyte stage, but myeloperoxidase mRNA levels disappeared earlier than do neutrophil elastase mRNA levels, suggesting that expression of these genes may be differently controlled. In comparison, lactoferrin mRNA transcripts were detected late in the neutrophil lineage, while β-globin mRNA was detected only in cells of the erythroid lineage. Together these observations suggest that the expression of the neutrophil elastase gene is likely under very tight control, and is likely different than that for other constituents of the neutrophil granules.

Original languageEnglish
Pages (from-to)833-845
Number of pages13
JournalJournal of Experimental Medicine
Volume169
Issue number3
Publication statusPublished - 1 Jan 1989
Externally publishedYes

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Leukocyte Elastase
Bone Marrow Cells
Cell Differentiation
Granulocyte Precursor Cells
Messenger RNA
Neutrophils
Genes
Peroxidase
Lactoferrin
Globins
Gene Expression
Complementary RNA
Serine Proteases
Cell Lineage
In Situ Hybridization
Bone Marrow

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fouret, P., Du Bois, R. M., Bernaudin, J. F., Takahashi, H., Ferrans, V. J., & Crystal, R. (1989). Expression of the neutrophil elastase gene during human bone marrow cell differentiation. Journal of Experimental Medicine, 169(3), 833-845.

Expression of the neutrophil elastase gene during human bone marrow cell differentiation. / Fouret, P.; Du Bois, R. M.; Bernaudin, J. F.; Takahashi, H.; Ferrans, V. J.; Crystal, Ronald.

In: Journal of Experimental Medicine, Vol. 169, No. 3, 01.01.1989, p. 833-845.

Research output: Contribution to journalArticle

Fouret, P, Du Bois, RM, Bernaudin, JF, Takahashi, H, Ferrans, VJ & Crystal, R 1989, 'Expression of the neutrophil elastase gene during human bone marrow cell differentiation', Journal of Experimental Medicine, vol. 169, no. 3, pp. 833-845.
Fouret P, Du Bois RM, Bernaudin JF, Takahashi H, Ferrans VJ, Crystal R. Expression of the neutrophil elastase gene during human bone marrow cell differentiation. Journal of Experimental Medicine. 1989 Jan 1;169(3):833-845.
Fouret, P. ; Du Bois, R. M. ; Bernaudin, J. F. ; Takahashi, H. ; Ferrans, V. J. ; Crystal, Ronald. / Expression of the neutrophil elastase gene during human bone marrow cell differentiation. In: Journal of Experimental Medicine. 1989 ; Vol. 169, No. 3. pp. 833-845.
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