Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney

Sunil Bhandari, Neil Watson, Ervine Long, Steve Sharpe, Wenwen Zhong, Shang Zhong Xu, Stephen L. Atkin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Somatostatin mediates inhibitory functions through five G protein-coupled somatostatin receptors (sst1-5). We used immunohistochemistry, immunofluorescence, and RT-PCR to determine the presence of somatostatin receptors sst1, sst2A, sst2B, sst3, sst4, and sst5 in normal and IgA nephropathy human kidney. All somatostatin receptors were detected in the thin tubules (distal convoluted tubules and loops of Henle) and thick tubules (proximal convoluted tubules) in the tissue sections from nephrectomy and biopsy samples. Immunopositive sst 1 and sst4 staining was more condensed in the cytoplasm of tubular epithelial cells. In normal kidney tissue sections, podocytes and mesangial cells in the glomeruli stained for sst1, sst2B, sst4 and sst5, and stained weakly for sst3. In IgA kidney tissue, the expression of somatostatin receptors was significantly increased with particular immmunopositive staining for sst1, sst 2B, sst4, and sst5 within glomeruli. In the epithelial cells, the staining for sst2B and sst4 in proximal tubules and sst1, sst2B, and sst5 in distal tubules was increased. The mRNA expression of sst1-5 was also detected by RT-PCR. Somatostatin and all five receptor subtypes were ubiquitously distributed in normal kidney and IgA nephropathy. The increased expression of somatostatin receptors in IgA nephropathy kidney might be the potential pathogenesis of inflammatory renal disease.

Original languageEnglish
Pages (from-to)733-743
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume56
Issue number8
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

Fingerprint

Kidney Diseases
Somatostatin
Kidney
Somatostatin Receptors
Immunoglobulin A
Staining and Labeling
Epithelial Cells
Loop of Henle
Podocytes
Polymerase Chain Reaction
Mesangial Cells
G-Protein-Coupled Receptors
Nephrectomy
Fluorescent Antibody Technique
somatostatin receptor type 1
Cytoplasm
Immunohistochemistry
Biopsy
Messenger RNA

Keywords

  • Human kidney
  • IgA nephropathy
  • Mesangial cells
  • Podocytes
  • Somatostatin
  • Somatostatin receptors
  • Tubule

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney. / Bhandari, Sunil; Watson, Neil; Long, Ervine; Sharpe, Steve; Zhong, Wenwen; Xu, Shang Zhong; Atkin, Stephen L.

In: Journal of Histochemistry and Cytochemistry, Vol. 56, No. 8, 08.2008, p. 733-743.

Research output: Contribution to journalArticle

Bhandari, Sunil ; Watson, Neil ; Long, Ervine ; Sharpe, Steve ; Zhong, Wenwen ; Xu, Shang Zhong ; Atkin, Stephen L. / Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney. In: Journal of Histochemistry and Cytochemistry. 2008 ; Vol. 56, No. 8. pp. 733-743.
@article{fe61a31c8b7c4f0bbfd2c0254d35ccd5,
title = "Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney",
abstract = "Somatostatin mediates inhibitory functions through five G protein-coupled somatostatin receptors (sst1-5). We used immunohistochemistry, immunofluorescence, and RT-PCR to determine the presence of somatostatin receptors sst1, sst2A, sst2B, sst3, sst4, and sst5 in normal and IgA nephropathy human kidney. All somatostatin receptors were detected in the thin tubules (distal convoluted tubules and loops of Henle) and thick tubules (proximal convoluted tubules) in the tissue sections from nephrectomy and biopsy samples. Immunopositive sst 1 and sst4 staining was more condensed in the cytoplasm of tubular epithelial cells. In normal kidney tissue sections, podocytes and mesangial cells in the glomeruli stained for sst1, sst2B, sst4 and sst5, and stained weakly for sst3. In IgA kidney tissue, the expression of somatostatin receptors was significantly increased with particular immmunopositive staining for sst1, sst 2B, sst4, and sst5 within glomeruli. In the epithelial cells, the staining for sst2B and sst4 in proximal tubules and sst1, sst2B, and sst5 in distal tubules was increased. The mRNA expression of sst1-5 was also detected by RT-PCR. Somatostatin and all five receptor subtypes were ubiquitously distributed in normal kidney and IgA nephropathy. The increased expression of somatostatin receptors in IgA nephropathy kidney might be the potential pathogenesis of inflammatory renal disease.",
keywords = "Human kidney, IgA nephropathy, Mesangial cells, Podocytes, Somatostatin, Somatostatin receptors, Tubule",
author = "Sunil Bhandari and Neil Watson and Ervine Long and Steve Sharpe and Wenwen Zhong and Xu, {Shang Zhong} and Atkin, {Stephen L.}",
year = "2008",
month = "8",
doi = "10.1369/jhc.2008.950998",
language = "English",
volume = "56",
pages = "733--743",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "8",

}

TY - JOUR

T1 - Expression of somatostatin and somatostatin receptor subtypes 1-5 in human normal and diseased kidney

AU - Bhandari, Sunil

AU - Watson, Neil

AU - Long, Ervine

AU - Sharpe, Steve

AU - Zhong, Wenwen

AU - Xu, Shang Zhong

AU - Atkin, Stephen L.

PY - 2008/8

Y1 - 2008/8

N2 - Somatostatin mediates inhibitory functions through five G protein-coupled somatostatin receptors (sst1-5). We used immunohistochemistry, immunofluorescence, and RT-PCR to determine the presence of somatostatin receptors sst1, sst2A, sst2B, sst3, sst4, and sst5 in normal and IgA nephropathy human kidney. All somatostatin receptors were detected in the thin tubules (distal convoluted tubules and loops of Henle) and thick tubules (proximal convoluted tubules) in the tissue sections from nephrectomy and biopsy samples. Immunopositive sst 1 and sst4 staining was more condensed in the cytoplasm of tubular epithelial cells. In normal kidney tissue sections, podocytes and mesangial cells in the glomeruli stained for sst1, sst2B, sst4 and sst5, and stained weakly for sst3. In IgA kidney tissue, the expression of somatostatin receptors was significantly increased with particular immmunopositive staining for sst1, sst 2B, sst4, and sst5 within glomeruli. In the epithelial cells, the staining for sst2B and sst4 in proximal tubules and sst1, sst2B, and sst5 in distal tubules was increased. The mRNA expression of sst1-5 was also detected by RT-PCR. Somatostatin and all five receptor subtypes were ubiquitously distributed in normal kidney and IgA nephropathy. The increased expression of somatostatin receptors in IgA nephropathy kidney might be the potential pathogenesis of inflammatory renal disease.

AB - Somatostatin mediates inhibitory functions through five G protein-coupled somatostatin receptors (sst1-5). We used immunohistochemistry, immunofluorescence, and RT-PCR to determine the presence of somatostatin receptors sst1, sst2A, sst2B, sst3, sst4, and sst5 in normal and IgA nephropathy human kidney. All somatostatin receptors were detected in the thin tubules (distal convoluted tubules and loops of Henle) and thick tubules (proximal convoluted tubules) in the tissue sections from nephrectomy and biopsy samples. Immunopositive sst 1 and sst4 staining was more condensed in the cytoplasm of tubular epithelial cells. In normal kidney tissue sections, podocytes and mesangial cells in the glomeruli stained for sst1, sst2B, sst4 and sst5, and stained weakly for sst3. In IgA kidney tissue, the expression of somatostatin receptors was significantly increased with particular immmunopositive staining for sst1, sst 2B, sst4, and sst5 within glomeruli. In the epithelial cells, the staining for sst2B and sst4 in proximal tubules and sst1, sst2B, and sst5 in distal tubules was increased. The mRNA expression of sst1-5 was also detected by RT-PCR. Somatostatin and all five receptor subtypes were ubiquitously distributed in normal kidney and IgA nephropathy. The increased expression of somatostatin receptors in IgA nephropathy kidney might be the potential pathogenesis of inflammatory renal disease.

KW - Human kidney

KW - IgA nephropathy

KW - Mesangial cells

KW - Podocytes

KW - Somatostatin

KW - Somatostatin receptors

KW - Tubule

UR - http://www.scopus.com/inward/record.url?scp=47249095867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47249095867&partnerID=8YFLogxK

U2 - 10.1369/jhc.2008.950998

DO - 10.1369/jhc.2008.950998

M3 - Article

C2 - 18443363

AN - SCOPUS:47249095867

VL - 56

SP - 733

EP - 743

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 8

ER -