Expression of NPR-B in neurons of the dorsal root ganglia of the rat

Essam Mohamed, Jean Pierre Bellier, Ikuo Tooyama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

C-type natriuretic peptide (CNP) is an abundant neuropeptide in the central nervous system, which exerts its physiological effects through natriuretic peptide receptor B (NPR-B). Recently, the CNP/NPR-B system has been recognized as an important regulator for the development of sensory axons. The dorsal root ganglion (DRG) contains neurons transmitting several kinds of spinal sensory stimuli to the central nervous system. In this study, we characterized NPR-B receptor expression in the rat DRG, using reverse transcription-polymerase chain reaction, Western blotting and immunohistochemistry. Immunostaining revealed that NPR-B was expressed in neuronal cell bodies and processes of the DRG, with NPR-B immunoreactivity mainly prominent in small and medium-sized DRG neurons. Double-immunolabeling showed that NPR-B was expressed in calcitonin gene-related peptide- and isolectin B4-positive neurons. Furthermore, NPR-B expression was co-localized with calcitonin gene-related peptide in the dorsal horn of the spinal cord. Together, our data suggest that the natriuretic peptides may perform several biological actions on sensory neurons via their binding to NPR-B in the DRG.

Original languageEnglish
Pages (from-to)56-61
Number of pages6
JournalPeptides
Volume43
DOIs
Publication statusPublished - 1 May 2013
Externally publishedYes

Fingerprint

Spinal Ganglia
Neurons
Rats
C-Type Natriuretic Peptide
Calcitonin Gene-Related Peptide
Neurology
Central Nervous System
Natriuretic Peptides
atrial natriuretic factor receptor B
Polymerase chain reaction
Sensory Receptor Cells
Transcription
Neuropeptides
Lectins
Reverse Transcription
Axons
Western Blotting
Immunohistochemistry
Cells
Polymerase Chain Reaction

Keywords

  • CG-B
  • CGRP
  • DRG
  • IB4
  • Immunohistochemistry
  • Sensory neurons

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Expression of NPR-B in neurons of the dorsal root ganglia of the rat. / Mohamed, Essam; Bellier, Jean Pierre; Tooyama, Ikuo.

In: Peptides, Vol. 43, 01.05.2013, p. 56-61.

Research output: Contribution to journalArticle

Mohamed, Essam ; Bellier, Jean Pierre ; Tooyama, Ikuo. / Expression of NPR-B in neurons of the dorsal root ganglia of the rat. In: Peptides. 2013 ; Vol. 43. pp. 56-61.
@article{5d0e7f5ae14041b7a5bfd823243db926,
title = "Expression of NPR-B in neurons of the dorsal root ganglia of the rat",
abstract = "C-type natriuretic peptide (CNP) is an abundant neuropeptide in the central nervous system, which exerts its physiological effects through natriuretic peptide receptor B (NPR-B). Recently, the CNP/NPR-B system has been recognized as an important regulator for the development of sensory axons. The dorsal root ganglion (DRG) contains neurons transmitting several kinds of spinal sensory stimuli to the central nervous system. In this study, we characterized NPR-B receptor expression in the rat DRG, using reverse transcription-polymerase chain reaction, Western blotting and immunohistochemistry. Immunostaining revealed that NPR-B was expressed in neuronal cell bodies and processes of the DRG, with NPR-B immunoreactivity mainly prominent in small and medium-sized DRG neurons. Double-immunolabeling showed that NPR-B was expressed in calcitonin gene-related peptide- and isolectin B4-positive neurons. Furthermore, NPR-B expression was co-localized with calcitonin gene-related peptide in the dorsal horn of the spinal cord. Together, our data suggest that the natriuretic peptides may perform several biological actions on sensory neurons via their binding to NPR-B in the DRG.",
keywords = "CG-B, CGRP, DRG, IB4, Immunohistochemistry, Sensory neurons",
author = "Essam Mohamed and Bellier, {Jean Pierre} and Ikuo Tooyama",
year = "2013",
month = "5",
day = "1",
doi = "10.1016/j.peptides.2013.02.013",
language = "English",
volume = "43",
pages = "56--61",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Expression of NPR-B in neurons of the dorsal root ganglia of the rat

AU - Mohamed, Essam

AU - Bellier, Jean Pierre

AU - Tooyama, Ikuo

PY - 2013/5/1

Y1 - 2013/5/1

N2 - C-type natriuretic peptide (CNP) is an abundant neuropeptide in the central nervous system, which exerts its physiological effects through natriuretic peptide receptor B (NPR-B). Recently, the CNP/NPR-B system has been recognized as an important regulator for the development of sensory axons. The dorsal root ganglion (DRG) contains neurons transmitting several kinds of spinal sensory stimuli to the central nervous system. In this study, we characterized NPR-B receptor expression in the rat DRG, using reverse transcription-polymerase chain reaction, Western blotting and immunohistochemistry. Immunostaining revealed that NPR-B was expressed in neuronal cell bodies and processes of the DRG, with NPR-B immunoreactivity mainly prominent in small and medium-sized DRG neurons. Double-immunolabeling showed that NPR-B was expressed in calcitonin gene-related peptide- and isolectin B4-positive neurons. Furthermore, NPR-B expression was co-localized with calcitonin gene-related peptide in the dorsal horn of the spinal cord. Together, our data suggest that the natriuretic peptides may perform several biological actions on sensory neurons via their binding to NPR-B in the DRG.

AB - C-type natriuretic peptide (CNP) is an abundant neuropeptide in the central nervous system, which exerts its physiological effects through natriuretic peptide receptor B (NPR-B). Recently, the CNP/NPR-B system has been recognized as an important regulator for the development of sensory axons. The dorsal root ganglion (DRG) contains neurons transmitting several kinds of spinal sensory stimuli to the central nervous system. In this study, we characterized NPR-B receptor expression in the rat DRG, using reverse transcription-polymerase chain reaction, Western blotting and immunohistochemistry. Immunostaining revealed that NPR-B was expressed in neuronal cell bodies and processes of the DRG, with NPR-B immunoreactivity mainly prominent in small and medium-sized DRG neurons. Double-immunolabeling showed that NPR-B was expressed in calcitonin gene-related peptide- and isolectin B4-positive neurons. Furthermore, NPR-B expression was co-localized with calcitonin gene-related peptide in the dorsal horn of the spinal cord. Together, our data suggest that the natriuretic peptides may perform several biological actions on sensory neurons via their binding to NPR-B in the DRG.

KW - CG-B

KW - CGRP

KW - DRG

KW - IB4

KW - Immunohistochemistry

KW - Sensory neurons

UR - http://www.scopus.com/inward/record.url?scp=84875291445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875291445&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2013.02.013

DO - 10.1016/j.peptides.2013.02.013

M3 - Article

VL - 43

SP - 56

EP - 61

JO - Peptides

JF - Peptides

SN - 0196-9781

ER -