Expression of natriuretic peptide-activated guanylate cyclases by cholinergic and dopaminergic amacrine cells of the rat retina

Essam Mohamed, Chiaki Masuda, Ikuo Tooyama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recently, the natriuretic peptides were detected in the cholinergic and dopaminergic amacrine cells of the retina. We performed immunofluorescence labeling of rat retinal sections to examine the immunoreactivity of natriuretic peptide-activated guanylate cyclases (NPR-A and NPR-B) in the rat retina, in particular whether they were localized to dopaminergic and cholinergic amacrine cells. NPR-A and NPR-B immunoreactivity was detected in several layers of the retina including amacrine cells. In amacrine cells, both NPR-A and NPR-B were co-localized with tyrosine hydroxylase, a marker of dopaminergic cells. NPR-B, but not NPR-A, was localized to amacrine cells expressing choline acetyltransferase (ChAT), a marker of cholinergic cells. These findings suggest that natriuretic peptides have different regulatory systems in dopaminergic and cholinergic amacrine cells in rat retina.

Original languageEnglish
Pages (from-to)622-628
Number of pages7
JournalPeptides
Volume29
Issue number4
DOIs
Publication statusPublished - 1 Apr 2008
Externally publishedYes

Fingerprint

Amacrine Cells
Natriuretic Peptides
Guanylate Cyclase
Cholinergic Agents
Retina
Rats
Cells
Choline O-Acetyltransferase
Tyrosine 3-Monooxygenase
Labeling
Fluorescent Antibody Technique

Keywords

  • Choline acetyltransferase
  • Immunofluorescence
  • NPR-A
  • NPR-B
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Expression of natriuretic peptide-activated guanylate cyclases by cholinergic and dopaminergic amacrine cells of the rat retina. / Mohamed, Essam; Masuda, Chiaki; Tooyama, Ikuo.

In: Peptides, Vol. 29, No. 4, 01.04.2008, p. 622-628.

Research output: Contribution to journalArticle

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