Expression of microRNA in follicular fluid in women with and without PCOS

Alexandra E. Butler, Vimal Ramachandran, Shahina Hayat, Soha R. Dargham, Thomas Keith Cunningham, Manasi Benurwar, Thozhukat Sathyapalan, S. Hani Najafi-Shoushtari, Stephen L. Atkin

Research output: Contribution to journalArticle

Abstract

Several studies have shown the expression of small non-coding microRNA (miRNA) changes in PCOS and their expression in follicular fluid has been described, though the number of studies remains small. In this prospective cohort study, miRNA were measured using quantitative polymerase chain reaction (qPCR) in 29 weight and aged matched anovulatory women with PCOS and 30 women without from follicular fluid taken at the time of oocyte retrieval who were undergoing in vitro fertilization (IVF); miRNA levels were determined from a miRNA data set. 176 miRNA were detected, of which 29 differed significantly between normal women and PCOS women. Of these, the top 7 (p < 0.015) were miR-381-3p, miR-199b-5p, miR-93-3p, miR-361-3p, miR-127-3p, miR-382-5p, miR-425-3p. In PCOS, miR-382-5p correlated with age and free androgen index (FAI), miR-199b-5p correlated with anti-mullerian hormone (AMH) and miR-93-3p correlated with C-reactive protein (CRP). In normal controls, miR-127-3p, miR-382-5p and miR-425-3p correlated with the fertilisation rate; miR-127-3p correlated with insulin resistance and miR-381-3p correlated with FAI. Ingenuity pathway assessment revealed that 12 of the significantly altered miRNA related to reproductive pathways, 12 miRNA related to the inflammatory disease pathway and 6 were implicated in benign pelvic disease. MiRNAs differed in the follicular fluid between PCOS and normal control women, correlating with age, FAI, inflammation and AMH in PCOS, and with BMI, fertilization rate (3 miRNA), insulin resistance, FAI and inflammation in control women, according to Ingenuity Pathway Analysis.

Original languageEnglish
Article number16306
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Follicular Fluid
MicroRNAs
Androgens
Anti-Mullerian Hormone
Fertilization
Insulin Resistance
Inflammation
Oocyte Retrieval
Fertilization in Vitro
C-Reactive Protein
Cohort Studies
Prospective Studies
Weights and Measures
Polymerase Chain Reaction

ASJC Scopus subject areas

  • General

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Expression of microRNA in follicular fluid in women with and without PCOS. / Butler, Alexandra E.; Ramachandran, Vimal; Hayat, Shahina; Dargham, Soha R.; Cunningham, Thomas Keith; Benurwar, Manasi; Sathyapalan, Thozhukat; Najafi-Shoushtari, S. Hani; Atkin, Stephen L.

In: Scientific reports, Vol. 9, No. 1, 16306, 01.12.2019.

Research output: Contribution to journalArticle

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abstract = "Several studies have shown the expression of small non-coding microRNA (miRNA) changes in PCOS and their expression in follicular fluid has been described, though the number of studies remains small. In this prospective cohort study, miRNA were measured using quantitative polymerase chain reaction (qPCR) in 29 weight and aged matched anovulatory women with PCOS and 30 women without from follicular fluid taken at the time of oocyte retrieval who were undergoing in vitro fertilization (IVF); miRNA levels were determined from a miRNA data set. 176 miRNA were detected, of which 29 differed significantly between normal women and PCOS women. Of these, the top 7 (p < 0.015) were miR-381-3p, miR-199b-5p, miR-93-3p, miR-361-3p, miR-127-3p, miR-382-5p, miR-425-3p. In PCOS, miR-382-5p correlated with age and free androgen index (FAI), miR-199b-5p correlated with anti-mullerian hormone (AMH) and miR-93-3p correlated with C-reactive protein (CRP). In normal controls, miR-127-3p, miR-382-5p and miR-425-3p correlated with the fertilisation rate; miR-127-3p correlated with insulin resistance and miR-381-3p correlated with FAI. Ingenuity pathway assessment revealed that 12 of the significantly altered miRNA related to reproductive pathways, 12 miRNA related to the inflammatory disease pathway and 6 were implicated in benign pelvic disease. MiRNAs differed in the follicular fluid between PCOS and normal control women, correlating with age, FAI, inflammation and AMH in PCOS, and with BMI, fertilization rate (3 miRNA), insulin resistance, FAI and inflammation in control women, according to Ingenuity Pathway Analysis.",
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AU - Cunningham, Thomas Keith

AU - Benurwar, Manasi

AU - Sathyapalan, Thozhukat

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AU - Atkin, Stephen L.

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