Expression of fibrinogen E-fragment and fibrin E-fragment is inhibited in the human infiltrating ductal carcinoma of the breast: The two-dimensional electrophoresis and MALDI-TOF-mass spectrometry analyses

Karim Chahed, Maria Kabbage, Laurence Ehret-Sabatier, Christelle Lemaitre-Guillier, Sami Remadi, Johan Hoebeke, Lotfi Chouchane

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In the present study, total proteins from a tissue of an infiltrating ductal carcinoma of the breast (IDCA) were compared by the two-dimensional electrophoresis (2D-PAGE) to proteins from an adjacent non-neoplastic breast tissue. Analysis of multiple gels for each sample identified nine proteins present in the tumor sample that were less present in the matched normal adjacent breast tissue and four proteins present at higher levels in the normal tissue. The altered proteins were identified by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and search in protein databases. Protein disulfide isomerase, BiP protein, calreticulin, cathepsin D, inorganic pyrophosphatase, vimentin, apolipoprotein Al precursor, tropomyosin 4 and B5-tubulin were identified as being significantly over-expressed in the IDCA with regard to the normal tissue. The expression of fibrinogen Efragment (known as anti-angiogenic factor) as well as of fibrin E, Pro2619 and actinGl was found to be inhibited in the tumor sample. The identified proteins might play an important role during malignant transformation, breast cancer progression, and angiogenesis as well as in cellular signaling. This study demonstrates quantitative and qualitative changes in protein abundance between IDCA and normal tissue. The identification of these differentially expressed proteins could lead to a better understanding of the molecular events linked to breast cancer progression.

Original languageEnglish
Pages (from-to)1425-1431
Number of pages7
JournalInternational journal of oncology
Volume27
Issue number5
DOIs
Publication statusPublished - Nov 2005

Keywords

  • Breast cancer
  • Proteomics
  • Two dimensional gel electrophoresis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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