Expression of B7-H1 in breast cancer patients is strongly associated with high proliferative Ki-67-expressing tumor cells

Hazem Ghebeh, Asma Tulbah, Shamayel Mohammed, Naser Elkum, Suad M. Bin Amer, Taher Al-Tweigeri, Said Dermime

Research output: Contribution to journalArticle

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Abstract

B7-H1, a coinhibitory molecule, plays a role in immune escape of tumors. We have shown previously the expression of this molecule in breast cancer patients and demonstrated its association with high histological grade, progesterone and estrogen receptor negative status, all of which are known to have direct impact on cell proliferation. In the present work, we investigated the effect of proliferation, as measured by Ki-67 and mitotic count, on the induction of B7-H1. We used H&E stained sections to score for mitotic count in 69 breast cancer patients. Immunohistochemistry was used to investigate B7-H1 and Ki-67 expression. The relationship between B7-H1 induction and cell proliferation was further investigated in primary cultured cells. B7-H1 expression was recorded in patients with a high mitotic index (p = 0.007). There was a high significant correlation between B7-H1 expression and the presence of the proliferative marker Ki-67 (p < 0.001) indicating the association of proliferation with B7-H1 induction. Furthermore, B7-H1 was gradually induced in proliferating cells of 8/8 primary cell lines as measured by Ki-67 expression. Finally, B7-H1 was downregulated in quiescent cells and upregulated in cells stimulated with a mitogen confirming the association of proliferation with the induction of B7-H1. We have shown for the first time a direct association between proliferation and the expression of B7-H1 in breast cancer patients. The relationship between B7-H1 induction and cell proliferation was also thoroughly investigated in vitro, in which a strong link between B7-H1 expression and the presence of the proliferative Ki-67 marker was clearly demonstrated.

Original languageEnglish
Pages (from-to)751-758
Number of pages8
JournalInternational Journal of Cancer
Volume121
Issue number4
DOIs
Publication statusPublished - 15 Aug 2007
Externally publishedYes

Fingerprint

Breast Neoplasms
Cell Proliferation
Neoplasms
Tumor Escape
Mitotic Index
Progesterone Receptors
Mitogens
Estrogen Receptors
Cultured Cells
Down-Regulation
Immunohistochemistry
Cell Line
In Vitro Techniques

Keywords

  • B7-H1
  • Breast cancer
  • Ki-67
  • Mitotic index
  • PD-L1
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Expression of B7-H1 in breast cancer patients is strongly associated with high proliferative Ki-67-expressing tumor cells. / Ghebeh, Hazem; Tulbah, Asma; Mohammed, Shamayel; Elkum, Naser; Bin Amer, Suad M.; Al-Tweigeri, Taher; Dermime, Said.

In: International Journal of Cancer, Vol. 121, No. 4, 15.08.2007, p. 751-758.

Research output: Contribution to journalArticle

Ghebeh, Hazem ; Tulbah, Asma ; Mohammed, Shamayel ; Elkum, Naser ; Bin Amer, Suad M. ; Al-Tweigeri, Taher ; Dermime, Said. / Expression of B7-H1 in breast cancer patients is strongly associated with high proliferative Ki-67-expressing tumor cells. In: International Journal of Cancer. 2007 ; Vol. 121, No. 4. pp. 751-758.
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abstract = "B7-H1, a coinhibitory molecule, plays a role in immune escape of tumors. We have shown previously the expression of this molecule in breast cancer patients and demonstrated its association with high histological grade, progesterone and estrogen receptor negative status, all of which are known to have direct impact on cell proliferation. In the present work, we investigated the effect of proliferation, as measured by Ki-67 and mitotic count, on the induction of B7-H1. We used H&E stained sections to score for mitotic count in 69 breast cancer patients. Immunohistochemistry was used to investigate B7-H1 and Ki-67 expression. The relationship between B7-H1 induction and cell proliferation was further investigated in primary cultured cells. B7-H1 expression was recorded in patients with a high mitotic index (p = 0.007). There was a high significant correlation between B7-H1 expression and the presence of the proliferative marker Ki-67 (p < 0.001) indicating the association of proliferation with B7-H1 induction. Furthermore, B7-H1 was gradually induced in proliferating cells of 8/8 primary cell lines as measured by Ki-67 expression. Finally, B7-H1 was downregulated in quiescent cells and upregulated in cells stimulated with a mitogen confirming the association of proliferation with the induction of B7-H1. We have shown for the first time a direct association between proliferation and the expression of B7-H1 in breast cancer patients. The relationship between B7-H1 induction and cell proliferation was also thoroughly investigated in vitro, in which a strong link between B7-H1 expression and the presence of the proliferative Ki-67 marker was clearly demonstrated.",
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AU - Mohammed, Shamayel

AU - Elkum, Naser

AU - Bin Amer, Suad M.

AU - Al-Tweigeri, Taher

AU - Dermime, Said

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