Expression and localization of transient receptor potential channels in the bovine uterus epithelium throughout the estrous cycle

Maryam Ghavideldarestani, Alexandra E. Butler, Sadegh Shirian, Stephen Atkin

Research output: Contribution to journalArticle

Abstract

Transient receptor potential (TRP) channels are expressed in the endometrium but it is unknown if they are modulated through the estrous cycle (EC). This study was undertaken to identify the modulation of the TRPC gene and protein isoforms in bovine uterine epithelium, as a model for human, throughout the EC. Changes in the expression of TRPC genes in bovine uterine epithelium throughout the EC were measured using Real-Time PCR, while immunohistochemistry and immunocytochemistry were used to determine the localization of these channels. Out of the 7 members of the TRPC family, TRPC1, 2, 3, 4 and 6 genes were expressed in bovine uterine epithelial tissue and TRPC 5 and 7 were not. Gene expression levels of all TRPC isoforms underwent cyclical changes throughout the EC. Moreover, cyclical changes were detected in the protein levels of TRPC1 and TRPC6 throughout the EC. These findings show that TRPC channels are modulated through the EC and therefore may have a role in reproductive events.

Original languageEnglish
JournalMolecular Biology Reports
DOIs
Publication statusPublished - 1 Jan 2019

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Keywords

  • Calcium
  • Endometrium
  • Sex hormones
  • TRPC channels

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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