Expression and localization of transient receptor potential channels in the bovine uterus epithelium throughout the estrous cycle

Maryam Ghavideldarestani, Alexandra E. Butler, Sadegh Shirian, Stephen Atkin

Research output: Contribution to journalArticle

Abstract

Transient receptor potential (TRP) channels are expressed in the endometrium but it is unknown if they are modulated through the estrous cycle (EC). This study was undertaken to identify the modulation of the TRPC gene and protein isoforms in bovine uterine epithelium, as a model for human, throughout the EC. Changes in the expression of TRPC genes in bovine uterine epithelium throughout the EC were measured using Real-Time PCR, while immunohistochemistry and immunocytochemistry were used to determine the localization of these channels. Out of the 7 members of the TRPC family, TRPC1, 2, 3, 4 and 6 genes were expressed in bovine uterine epithelial tissue and TRPC 5 and 7 were not. Gene expression levels of all TRPC isoforms underwent cyclical changes throughout the EC. Moreover, cyclical changes were detected in the protein levels of TRPC1 and TRPC6 throughout the EC. These findings show that TRPC channels are modulated through the EC and therefore may have a role in reproductive events.

Original languageEnglish
JournalMolecular Biology Reports
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Transient Receptor Potential Channels
Estrous Cycle
Uterus
Epithelium
Protein Isoforms
Immunohistochemistry
Gene Expression
Endometrium
Real-Time Polymerase Chain Reaction
Proteins
Genes

Keywords

  • Calcium
  • Endometrium
  • Sex hormones
  • TRPC channels

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Expression and localization of transient receptor potential channels in the bovine uterus epithelium throughout the estrous cycle. / Ghavideldarestani, Maryam; Butler, Alexandra E.; Shirian, Sadegh; Atkin, Stephen.

In: Molecular Biology Reports, 01.01.2019.

Research output: Contribution to journalArticle

@article{c550875bdf1449e893120b4813f8960a,
title = "Expression and localization of transient receptor potential channels in the bovine uterus epithelium throughout the estrous cycle",
abstract = "Transient receptor potential (TRP) channels are expressed in the endometrium but it is unknown if they are modulated through the estrous cycle (EC). This study was undertaken to identify the modulation of the TRPC gene and protein isoforms in bovine uterine epithelium, as a model for human, throughout the EC. Changes in the expression of TRPC genes in bovine uterine epithelium throughout the EC were measured using Real-Time PCR, while immunohistochemistry and immunocytochemistry were used to determine the localization of these channels. Out of the 7 members of the TRPC family, TRPC1, 2, 3, 4 and 6 genes were expressed in bovine uterine epithelial tissue and TRPC 5 and 7 were not. Gene expression levels of all TRPC isoforms underwent cyclical changes throughout the EC. Moreover, cyclical changes were detected in the protein levels of TRPC1 and TRPC6 throughout the EC. These findings show that TRPC channels are modulated through the EC and therefore may have a role in reproductive events.",
keywords = "Calcium, Endometrium, Sex hormones, TRPC channels",
author = "Maryam Ghavideldarestani and Butler, {Alexandra E.} and Sadegh Shirian and Stephen Atkin",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s11033-019-04857-w",
language = "English",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Expression and localization of transient receptor potential channels in the bovine uterus epithelium throughout the estrous cycle

AU - Ghavideldarestani, Maryam

AU - Butler, Alexandra E.

AU - Shirian, Sadegh

AU - Atkin, Stephen

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Transient receptor potential (TRP) channels are expressed in the endometrium but it is unknown if they are modulated through the estrous cycle (EC). This study was undertaken to identify the modulation of the TRPC gene and protein isoforms in bovine uterine epithelium, as a model for human, throughout the EC. Changes in the expression of TRPC genes in bovine uterine epithelium throughout the EC were measured using Real-Time PCR, while immunohistochemistry and immunocytochemistry were used to determine the localization of these channels. Out of the 7 members of the TRPC family, TRPC1, 2, 3, 4 and 6 genes were expressed in bovine uterine epithelial tissue and TRPC 5 and 7 were not. Gene expression levels of all TRPC isoforms underwent cyclical changes throughout the EC. Moreover, cyclical changes were detected in the protein levels of TRPC1 and TRPC6 throughout the EC. These findings show that TRPC channels are modulated through the EC and therefore may have a role in reproductive events.

AB - Transient receptor potential (TRP) channels are expressed in the endometrium but it is unknown if they are modulated through the estrous cycle (EC). This study was undertaken to identify the modulation of the TRPC gene and protein isoforms in bovine uterine epithelium, as a model for human, throughout the EC. Changes in the expression of TRPC genes in bovine uterine epithelium throughout the EC were measured using Real-Time PCR, while immunohistochemistry and immunocytochemistry were used to determine the localization of these channels. Out of the 7 members of the TRPC family, TRPC1, 2, 3, 4 and 6 genes were expressed in bovine uterine epithelial tissue and TRPC 5 and 7 were not. Gene expression levels of all TRPC isoforms underwent cyclical changes throughout the EC. Moreover, cyclical changes were detected in the protein levels of TRPC1 and TRPC6 throughout the EC. These findings show that TRPC channels are modulated through the EC and therefore may have a role in reproductive events.

KW - Calcium

KW - Endometrium

KW - Sex hormones

KW - TRPC channels

UR - http://www.scopus.com/inward/record.url?scp=85065730636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065730636&partnerID=8YFLogxK

U2 - 10.1007/s11033-019-04857-w

DO - 10.1007/s11033-019-04857-w

M3 - Article

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

ER -