Expression and function of transient receptor potential channels in the female bovine reproductive tract

Maryam Ghavideldarestani, Stephen Atkin, Henry J. Leese, Roger G. Sturmey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function.

Original languageEnglish
Pages (from-to)551-561
Number of pages11
JournalTheriogenology
Volume86
Issue number2
DOIs
Publication statusPublished - 26 Oct 2015
Externally publishedYes

Fingerprint

Transient Receptor Potential Channels
Oviducts
oviducts
Calcium
calcium
estrous cycle
cattle
epithelium
Estrous Cycle
Epithelium
Progesterone
estradiol
Estradiol
progesterone
epithelial cells
Epithelial Cells
Estrus
homeostasis
Homeostasis
protein synthesis

Keywords

  • Bovine
  • Calcium
  • Epithelium
  • Oviduct
  • Sex hormones
  • TRPC channels

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Equine
  • Animal Science and Zoology

Cite this

Expression and function of transient receptor potential channels in the female bovine reproductive tract. / Ghavideldarestani, Maryam; Atkin, Stephen; Leese, Henry J.; Sturmey, Roger G.

In: Theriogenology, Vol. 86, No. 2, 26.10.2015, p. 551-561.

Research output: Contribution to journalArticle

Ghavideldarestani, Maryam ; Atkin, Stephen ; Leese, Henry J. ; Sturmey, Roger G. / Expression and function of transient receptor potential channels in the female bovine reproductive tract. In: Theriogenology. 2015 ; Vol. 86, No. 2. pp. 551-561.
@article{e1fb0e612b584231bb8dba83dbd2504a,
title = "Expression and function of transient receptor potential channels in the female bovine reproductive tract",
abstract = "The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function.",
keywords = "Bovine, Calcium, Epithelium, Oviduct, Sex hormones, TRPC channels",
author = "Maryam Ghavideldarestani and Stephen Atkin and Leese, {Henry J.} and Sturmey, {Roger G.}",
year = "2015",
month = "10",
day = "26",
doi = "10.1016/j.theriogenology.2016.02.005",
language = "English",
volume = "86",
pages = "551--561",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Expression and function of transient receptor potential channels in the female bovine reproductive tract

AU - Ghavideldarestani, Maryam

AU - Atkin, Stephen

AU - Leese, Henry J.

AU - Sturmey, Roger G.

PY - 2015/10/26

Y1 - 2015/10/26

N2 - The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function.

AB - The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function.

KW - Bovine

KW - Calcium

KW - Epithelium

KW - Oviduct

KW - Sex hormones

KW - TRPC channels

UR - http://www.scopus.com/inward/record.url?scp=84962574242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962574242&partnerID=8YFLogxK

U2 - 10.1016/j.theriogenology.2016.02.005

DO - 10.1016/j.theriogenology.2016.02.005

M3 - Article

AN - SCOPUS:84962574242

VL - 86

SP - 551

EP - 561

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 2

ER -