Expression and function of transient receptor potential channels in the female bovine reproductive tract

Maryam Ghavideldarestani, Stephen Atkin, Henry J. Leese, Roger G. Sturmey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function.

Original languageEnglish
Pages (from-to)551-561
Number of pages11
JournalTheriogenology
Volume86
Issue number2
DOIs
Publication statusPublished - 26 Oct 2015
Externally publishedYes

Keywords

  • Bovine
  • Calcium
  • Epithelium
  • Oviduct
  • Sex hormones
  • TRPC channels

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Equine
  • Animal Science and Zoology

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