Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis: Novel signals involved

Ilaria Guerriero, Maria Teresa De Angelis, Fulvio D'angelo, Rita Leveque, Eleonora Savignano, Luca Roberto, Valeria Lucci, Pellegrino Mazzone, Simona Laurino, Giovanni Storto, Anna Nardelli, Alessandro Sgambato, Michele Ceccarelli, Mario De Felice, Elena Amendola, Geppino Falco

Research output: Contribution to journalArticle

Abstract

Pancreatic organogenesis is a multistep process that requires the cooperation of several signaling pathways. In this context, the role of pancreatic mesenchyme is important to define the epithelium development; nevertheless, the precise space–temporal signaling activation still needs to be clarified. This study reports a dissection of the pancreatic embryogenesis, highlighting the molecular network surrounding the epithelium–mesenchyme interaction. To investigate this crosstalk, pancreatic epithelium and surrounding mesenchyme, at embryonic day 10.5, were collected through laser capture microdissection (LCM) and characterized based on their global gene expression. We performed a bioinformatic analysis to hypothesize crosstalk interactions, validating the most promising genes and verifying the precise localization of their expression in the compartments, by RNA in situ hybridization (ISH). Our analyses pointed out also the c-Met gene, a very well-known factor involved in stimulating motility, morphogenesis, and organ regeneration. We also highlighted the potential crosstalk between Versican (Vcan) and Syndecan4 (Sdc4) since these genes are involved in pancreatic tissue repair, strengthening the concept that the same signaling pathways required during pancreatic embryogenesis are also involved in tissue repair. This finding leads to novel strategies for obtaining functional pancreatic stem cells for cell replacement therapies.

Original languageEnglish
Article number4900
JournalInternational journal of molecular sciences
Volume20
Issue number19
DOIs
Publication statusPublished - 1 Oct 2019

Fingerprint

Mesoderm
Crosstalk
crosstalk
genes
Embryonic Development
epithelium
Genes
Microdissection
Repair
Syndecan-4
Epithelium
Versicans
Tissue
Laser Capture Microdissection
Dissection
dissection
locomotion
Organogenesis
stem cells
gene expression

Keywords

  • Bud
  • Embryonic stem cells
  • Laser microdissection
  • Mesenchymal stem cell
  • Pancreatic disorders
  • Pancreatic stem cells
  • Progenitor cells

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Guerriero, I., De Angelis, M. T., D'angelo, F., Leveque, R., Savignano, E., Roberto, L., ... Falco, G. (2019). Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis: Novel signals involved. International journal of molecular sciences, 20(19), [4900]. https://doi.org/10.3390/ijms20194900

Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis : Novel signals involved. / Guerriero, Ilaria; De Angelis, Maria Teresa; D'angelo, Fulvio; Leveque, Rita; Savignano, Eleonora; Roberto, Luca; Lucci, Valeria; Mazzone, Pellegrino; Laurino, Simona; Storto, Giovanni; Nardelli, Anna; Sgambato, Alessandro; Ceccarelli, Michele; De Felice, Mario; Amendola, Elena; Falco, Geppino.

In: International journal of molecular sciences, Vol. 20, No. 19, 4900, 01.10.2019.

Research output: Contribution to journalArticle

Guerriero, I, De Angelis, MT, D'angelo, F, Leveque, R, Savignano, E, Roberto, L, Lucci, V, Mazzone, P, Laurino, S, Storto, G, Nardelli, A, Sgambato, A, Ceccarelli, M, De Felice, M, Amendola, E & Falco, G 2019, 'Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis: Novel signals involved', International journal of molecular sciences, vol. 20, no. 19, 4900. https://doi.org/10.3390/ijms20194900
Guerriero, Ilaria ; De Angelis, Maria Teresa ; D'angelo, Fulvio ; Leveque, Rita ; Savignano, Eleonora ; Roberto, Luca ; Lucci, Valeria ; Mazzone, Pellegrino ; Laurino, Simona ; Storto, Giovanni ; Nardelli, Anna ; Sgambato, Alessandro ; Ceccarelli, Michele ; De Felice, Mario ; Amendola, Elena ; Falco, Geppino. / Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis : Novel signals involved. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 19.
@article{8957eee00b93488493cd9d19649a119a,
title = "Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis: Novel signals involved",
abstract = "Pancreatic organogenesis is a multistep process that requires the cooperation of several signaling pathways. In this context, the role of pancreatic mesenchyme is important to define the epithelium development; nevertheless, the precise space–temporal signaling activation still needs to be clarified. This study reports a dissection of the pancreatic embryogenesis, highlighting the molecular network surrounding the epithelium–mesenchyme interaction. To investigate this crosstalk, pancreatic epithelium and surrounding mesenchyme, at embryonic day 10.5, were collected through laser capture microdissection (LCM) and characterized based on their global gene expression. We performed a bioinformatic analysis to hypothesize crosstalk interactions, validating the most promising genes and verifying the precise localization of their expression in the compartments, by RNA in situ hybridization (ISH). Our analyses pointed out also the c-Met gene, a very well-known factor involved in stimulating motility, morphogenesis, and organ regeneration. We also highlighted the potential crosstalk between Versican (Vcan) and Syndecan4 (Sdc4) since these genes are involved in pancreatic tissue repair, strengthening the concept that the same signaling pathways required during pancreatic embryogenesis are also involved in tissue repair. This finding leads to novel strategies for obtaining functional pancreatic stem cells for cell replacement therapies.",
keywords = "Bud, Embryonic stem cells, Laser microdissection, Mesenchymal stem cell, Pancreatic disorders, Pancreatic stem cells, Progenitor cells",
author = "Ilaria Guerriero and {De Angelis}, {Maria Teresa} and Fulvio D'angelo and Rita Leveque and Eleonora Savignano and Luca Roberto and Valeria Lucci and Pellegrino Mazzone and Simona Laurino and Giovanni Storto and Anna Nardelli and Alessandro Sgambato and Michele Ceccarelli and {De Felice}, Mario and Elena Amendola and Geppino Falco",
year = "2019",
month = "10",
day = "1",
doi = "10.3390/ijms20194900",
language = "English",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "19",

}

TY - JOUR

T1 - Exploring the molecular crosstalk between pancreatic bud and mesenchyme in embryogenesis

T2 - Novel signals involved

AU - Guerriero, Ilaria

AU - De Angelis, Maria Teresa

AU - D'angelo, Fulvio

AU - Leveque, Rita

AU - Savignano, Eleonora

AU - Roberto, Luca

AU - Lucci, Valeria

AU - Mazzone, Pellegrino

AU - Laurino, Simona

AU - Storto, Giovanni

AU - Nardelli, Anna

AU - Sgambato, Alessandro

AU - Ceccarelli, Michele

AU - De Felice, Mario

AU - Amendola, Elena

AU - Falco, Geppino

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Pancreatic organogenesis is a multistep process that requires the cooperation of several signaling pathways. In this context, the role of pancreatic mesenchyme is important to define the epithelium development; nevertheless, the precise space–temporal signaling activation still needs to be clarified. This study reports a dissection of the pancreatic embryogenesis, highlighting the molecular network surrounding the epithelium–mesenchyme interaction. To investigate this crosstalk, pancreatic epithelium and surrounding mesenchyme, at embryonic day 10.5, were collected through laser capture microdissection (LCM) and characterized based on their global gene expression. We performed a bioinformatic analysis to hypothesize crosstalk interactions, validating the most promising genes and verifying the precise localization of their expression in the compartments, by RNA in situ hybridization (ISH). Our analyses pointed out also the c-Met gene, a very well-known factor involved in stimulating motility, morphogenesis, and organ regeneration. We also highlighted the potential crosstalk between Versican (Vcan) and Syndecan4 (Sdc4) since these genes are involved in pancreatic tissue repair, strengthening the concept that the same signaling pathways required during pancreatic embryogenesis are also involved in tissue repair. This finding leads to novel strategies for obtaining functional pancreatic stem cells for cell replacement therapies.

AB - Pancreatic organogenesis is a multistep process that requires the cooperation of several signaling pathways. In this context, the role of pancreatic mesenchyme is important to define the epithelium development; nevertheless, the precise space–temporal signaling activation still needs to be clarified. This study reports a dissection of the pancreatic embryogenesis, highlighting the molecular network surrounding the epithelium–mesenchyme interaction. To investigate this crosstalk, pancreatic epithelium and surrounding mesenchyme, at embryonic day 10.5, were collected through laser capture microdissection (LCM) and characterized based on their global gene expression. We performed a bioinformatic analysis to hypothesize crosstalk interactions, validating the most promising genes and verifying the precise localization of their expression in the compartments, by RNA in situ hybridization (ISH). Our analyses pointed out also the c-Met gene, a very well-known factor involved in stimulating motility, morphogenesis, and organ regeneration. We also highlighted the potential crosstalk between Versican (Vcan) and Syndecan4 (Sdc4) since these genes are involved in pancreatic tissue repair, strengthening the concept that the same signaling pathways required during pancreatic embryogenesis are also involved in tissue repair. This finding leads to novel strategies for obtaining functional pancreatic stem cells for cell replacement therapies.

KW - Bud

KW - Embryonic stem cells

KW - Laser microdissection

KW - Mesenchymal stem cell

KW - Pancreatic disorders

KW - Pancreatic stem cells

KW - Progenitor cells

UR - http://www.scopus.com/inward/record.url?scp=85073561143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073561143&partnerID=8YFLogxK

U2 - 10.3390/ijms20194900

DO - 10.3390/ijms20194900

M3 - Article

C2 - 31623299

AN - SCOPUS:85073561143

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 19

M1 - 4900

ER -